Fig 1: Detection of glutamate decarboxylase (GAD65) and glutamate decarboxylase antibody (GADAb-mono) mixed solutions using glass nanopores. (a) Schematic of mixed solution translocation through the nanopore. (b) Current signals generated by GAD65, GADAb-mono and their mixture passing through glass nanopores under voltage of 300 mV. (c–e) Histograms and fitted normal distributions of ΔI/I0 at 300 mV voltage for GAD65, GADAb-mono, and the mixed solution of GAD65 and GADAb-mono.
Fig 2: Detection of glutamate decarboxylase molecules using glass nanopores. (a) Three-dimensional image of GAD65 protein molecules [35]. (b) Current signals are generated by GAD65 molecules passing through glass nanopores under voltages of −300 mV to −500 mV; the signal is rotated 180° for easy observation. (c) GAD65 molecule translocation time under voltages of −300 mV to −500 mV. (d) Blockade current amplitude ratio ΔI/I0 of GAD65 molecules under voltages of −300 mV to −500 mV.
Fig 3: Transient molecular congestion phenomena in nanopores. (a) Simultaneous translocation of multiple glutamate decarboxylase molecules through a nanopore. (b) Current signatures corresponding to different translocation time events of GAD65 at −400 mV voltage. (c) Scatter plot of translocation time versus blockage current amplitude of GAD65 at −400 mV voltage.
Supplier Page from Abcam for Recombinant Human GAD65 protein