Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8) with a linear range of 4-31 ng/mL (QC tested).

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8): Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006 μg/mL (Routinely tested).

Human SIRP alpha, Mouse IgG1 Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.