Recombinant Human IGFBP3, His tagged IGFBP3-758H from Creative BioMart

Product Specs
ItemRecombinant Human IGFBP3, His tagged
CompanyCreative BioMart
Price Supplier Page View Company Product Page
Catalog NumberIGFBP3-758H
SpeciesHuman
Source/Expression SystemHuman Cells
NCBI Full Gene Nameinsulin like growth factor binding protein 3
NCBI Gene AliasesBP-53, IBP3
Gene NameIGFBP3, His tagged (Human)
Molecule NameIGFBP3
Conjugate/TagHis
ApplicationsFor research and further manufacturing use
Molecular WeightThe secreted recombinant human IGFBP3 comprises 280 amino acids with a predicted molecular mass of 31 kDa. As a result of glycosylation, the apparent molecular mass of rhIGFBP3 is approximately 40-45 kDa in SDS-PAGE under reducing conditions
Size1
Purity>90 % as determined by SDS-PAGE
Activity1. Measured by its binding ability in a functional ELISA. Immobilized human IGF2 at 10 µg/mL (100 µl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 18 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized human IGF1 at 10 µg/mL (100 µl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 24 ng/mL. 3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 0.05-0.2 µg/mL in the presence of 14 ng/mL human IGFII

Phone: 1-631-559-9269
1-516-512-3133

Email: info@creative-biomart.com

Recombinant Human IGFBP3, His tagged from Creative BioMart