Protein A Agarose High Flow Resin COP26 from Creative BioMart

Product Specs
ItemProtein A Agarose High Flow Resin
CompanyCreative BioMart
Price Supplier Page View Company Product Page
Catalog NumberCOP26
Gene NameProtein A Agarose High Flow Resin
FormatProduct Overview: RProtein A Agarose High Flow Resin is a classic protein A affinity medium for fast and efficient purification of monoclonal and polyclonal antibodies at any scale. The high specificity of rProtein A ligand to Fc domain of IgGs gives a high purification factor in a single chromatography step. Superior coupling chemistry ensures both high capacity and low leakage. RProtein A Agarose High Flow resin has also been used in immunoprecipitation (IP) procedures successfully.Description: RProtein A Agarose High Flow Resin is manufactured by immobilizing recombinant rProtein A ligand to highly cross-linked agarose matrix through stable bond formed by epoxy coupling chemistry. RProtein A ligand is derived from E. Coli fermentation and interacts with antibodies through two distinct binding events: the "classical" primary binding site on the Fc region of human IgG1, IgG2, and IgG4, and the "alternate" secondary binding site on the Fab region of human IgG, IgM, IgA, and IgE that contain heavy chains of the VH3 subfamily. RProtein A Agarose High Flow has been used for both excellent chromatography purification of poly and monoclonal antibodies from several species of mammals in one step, but also immunoprecipitation to purify and detect proteins or protein complexes indirectly through antibodies against the protein or protein complex of interest successfully.Features: Classic rProtein A resin suitable for different applications, including purification of poly or monoclonal antibodies of different species and subclasses and isolation of immune complex. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity.Specifications: Note: Determined at 10% breakthrough at 4min residence time. Delivered at room temperature, and recommended long-term storage at 2-8 °C

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Creative BioMart

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Protein A Agarose High Flow Resin from Creative BioMart

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Protein A Agarose High Flow Resin from Creative BioMart

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