Recombinant Mouse Fcgr4, His tagged Fcgr4-627M from Creative BioMart

Product Specs
ItemRecombinant Mouse Fcgr4, His tagged
CompanyCreative BioMart
Price Supplier Page View Company Product Page
Catalog NumberFcgr4-627M
SpeciesMouse
Source/Expression SystemHuman Cells
Gene NameFcgr4, His tagged (Mouse)
Conjugate/TagHis
NCBI Full Gene NameFc receptor, IgG, low affinity IV
NCBI Gene Aliases4833442P21Rik, CD16-2, FcgRIV, FcgammaRIV, Fcgr3a, Fcrl3
ApplicationsFor research and further manufacturing use
Molecule NameFcgr4
Molecular WeightThe recombinant mouse FCGR4 consists of 194 amino acids and has a predicted molecular mass of 22.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation
Size1
Purity>90 % as determined by SDS-PAGE
Activity1. Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR4 at 10 µg/ml (100 µl/well) can bind recombinant human IgG1 (Fc). The EC50 of human IgG1 (Fc) is 0.11-0.25 µg/ml. 2. Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Recombinant Mouse FCGR4 Protein, His Tag with an affinity constant of 3.43nM as determined in BLI assay (Sartorius Octet RED384).
3.Loaded Recombinant Mouse CD32B/Fcgr2b Protein, His Tag on His1K Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) with an affinity constant of 36.2nM as determined in BLI assay (Sartorius Octet RED384)(Routinely tested)

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Recombinant Mouse Fcgr4, His tagged from Creative BioMart