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Recombinant Human FCGR1A, His tagged
Recombinant Human FCGR1A, His tagged from Creative BioMart
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Creative BioMart for
Recombinant Human FCGR1A, His tagged
Description
Recombinant Human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus
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Product Specs
Item
Recombinant Human FCGR1A, His tagged
Company
Creative BioMart
Price
Supplier Page
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Catalog Number
FCGR1A-2224H
Species
Human
Source/Expression System
Human Cells
NCBI Full Gene Name
Fc gamma receptor Ia
NCBI Gene Aliases
CD64, CD64A, FCG1, FCGR1, FCRI, FcgammaRI, IGFR1
Gene Name
FCGR1A, His tagged (Human)
Conjugate/Tag
His
Applications
For research and further manufacturing use
Molecule Name
FCGR1A
Molecular Weight
The recombinant human CD64A consists of 284 amino acids after removal of the signal peptide and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD64A is approximately 45-60 kDa due to glycosylation
Size
1
Purity
>90 % as determined by SDS-PAGE
Activity
1. Immobilized Rituximab at 1 µg/mL can bind Human FcγRI / CD64 recombinant protein, with a linear range of 0.5-4.0 ng/mL. 2. Captured Human FcγRI / CD64 recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay (Biacore T200). 3. Captured Human FcγRI / CD64 recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 13.6 nM as determined in an SPR assay (Biacore T200). 4. Loaded Human FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay (ForteBio Octet Red384). 5. Loaded Human FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 4.7 nM as determined in a BLI assay (ForteBio Octet Red384). 6. Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRI / CD64 recombinant protein with an affinity constant of 0.38 nM as determined in a BLI assay (ForteBio Octet Red384)
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Creative BioMart
Phone
: 1-631-559-9269
1-516-512-3133
Email: info@creative-biomart.com
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Website:
https://www.creativebiomart.net/
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Recombinant Human FCGR1A, His tagged from Creative BioMart
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