Trypsin/Lys-C Mix, Mass Spec Grade V5072 from Promega

Product Specs
ItemTrypsin/Lys-C Mix, Mass Spec Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV5072
Size100microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) BOLD-100 treatment induces systemic lipid crosstalk in metastatic colorectal carcinoma patients in a combination treatment with FOLFOXiScienceSeptember 18, 2026Yasmin Borutzki, Gerhard Hagn, Andrea Bileck, Thomas Mohr, Mark Bazett, Malcolm Snow, Michelle Jones, Jim Pankovich, Christopher Gerner, Bernhard K. Keppler, et al.C4706 2-chloroacetamide (2-CAM),Alfa Aesar,Cat# 11462278 "Trypsin/Lys-C Mix, Mass Spec Grade",Promega GmbH,Cat# V5071 Isopropanol,VWR Chemicals,Cat# 20839 Trifluoroacetic acid (TFA),Sigma Aldrich,Cat# 302031 Empore™

(2) Method Matters: A Proteomics-Informed Framework for Selecting Extracellular Vesicle Isolation Methods for PlasmaJ Extracell BiolAugust 29, 2026Scheila Julia Werle, Marie Louise Nautrup Therkelsen, Chen Meng, Mads Grønborg, Lise Lotte Gluud, Dres Damgaardexchange to PBS. A buffer comprising 10% n‐Dodecyl β‐D‐Maltoside (DDM), Trypsin/Lys‐C (Promega, Cat# V5071), and 50 mM triethylammonium bicarbonate (TEAB) were added to the filter tube for the lysis of retained

(3) Members of the RhoA subfamily of GTPases suppress the stimulatory effect of the HPV E6 oncoprotein on the ubiquitin ligase E6APSci RepAugust 25, 2026Daniela Eichbichler, Jasmin Jansen, Nadja Eulich, Florian Stengel, Martin Scheffneriodoacetamide for 30 min at 23 °C in the dark. After dilution of SOD to 1%, Lys-C/trypsin mix (Promega V5071, Sequencing grade) was added at an E:S ratio of 1:50 and incubated for 18 h at 37 °C. The enzymatic

(4) Quantitative proteomic analysis of the anti-ferroptosis effect of a proprietary hydroxy-α-sanshool extract in human keratinocytes using a tandem mass tag labeling approachRSC AdvAugust 14, 2026Huifang Li, Xian Jiang, Ang Cai, Chang Liu, Hang Mawas then transferred to a new tube for digestion. Trypsin/Lys-C protease (Mass Spec Grade, Promega V5071) was added to the sample to a final volume of 125 µL, and the sample was incubated overnight at 37 °C

(5) Proteome Remodeling of a Carbapenem-Resistant Escherichia coli Strain upon Meropenem ExposureJournal of Proteome ResearchAugust 7, 2026Leonarda Acha Alarcon, Daniel Jaén-Luchoro, Francisco Salvà-Serra, Edward R. B. Moore, Ivan Mijakovic, Roger Karlssonbeads were resuspended in 50 mM TEAB, and proteins were digested with a Trypsin/Lys-C mix (1:25; ref. V5071; Promega; WI; USA) for 2 h, followed by trypsin digestion (1:50; ref. V5111; Promega; WI; USA) overnight.

(6) TRPA1 deficiency alters brown adipose tissue proteome and thermogenic gene expression during cold exposure in miceBiochem Biophys RepAugust 7, 2026Cristhian D. Sua-Cespedes, Otoniel Gonçalves Lima, Letícia Menezes Vasconcelos, Giovanna Zanetti, Miriane Avelino da Silva, Maria Nathalia Moraes, Guilherme Gomes, William Tadeu Festuccia, Ana Maria L. Castrucci, José Thalles LacerdaTween 20,Sigma-Aldrich,P2287 Urea,Merck,1.08487.1000 Iodoacetamide,Sigma-Aldrich,I6125 NH4HCO3,Sigma-Aldrich,A6141 Trypsin/LysC Mix,Promega,V5071 Trypsin,Promega,V5280 Acetonitrile,Sigma-Aldrich,900667 Trifluoroacetic acid,ThermoScientific,28901 C18

(7) Microcephaly-like phenotype triggered by novel reassortant and prototypic Oropouche virus strains in brain organoidsEBioMedicineJuly 30, 2026Gabrielle Brum, Vivian Grizente, Fábio Luís Lima Monteiro, Beatriz Luzia de Mello Lima Guimarães, Livia Goto-Silva, João Marcos de Azevedo Delou, Pedro Junior Pinheiro Mourão, Ismael Carlos da Silva Gomes, Enzo Oliveira Barone, Matheus Villanueva Andrade, et al.undesired surfactants followed by on-bead proteolysis (37 °C, overnight) with Trypsin/Lys-C (Promega, V5071) (enzyme/substrate ratio = 1:25). Following proteolysis, peptides were quantified using the Pierce Quantitative Fluorometric Peptide Assay

(8) Enrichment-free deep proteomics enables proteome-scale analysis of methionine oxidationDNA ResJuly 27, 2026Hiromasa Mitsui, Yusei Okuda, Ryo Konno, Daisuke Nakajima, Osamu Ohara, Yusuke Kawashimaneopentyl glycol with or without 10 mM methionine, followed by addition of 1 μg of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA), and mixed gently at 37 °C 16 h to digest proteins. The digested peptides

(9) Time-Resolved Label-Free Proteomics of SHK-1 Cells After Renibacterium salmoninarum Inoculation Reveals Early Host-Cell RemodelingInternational Journal of Molecular SciencesJune 26, 2026Jorge F. Beltrán, Jörn Bethke, Sandra Flores-Martin, Claudia A. Barrientos, Marcelo Aguilar, Adolfo Isla, Felipe Almendras, Marcos Mancilla, Alejandro J. Yañezwith 25 mM ammonium bicarbonate. Proteolytic digestion was carried out with sequencing-grade trypsin (#V5071, Promega, Madison, WI, USA) at a 1:50 protease-to-protein ratio (w/w) for 16 h at 37 °C. Digestion was

(10) A Micro-Quantitative and FFPE-Compatible Workflow for Immunohistochemistry-Guided Spatial Proteomic Analysis of Cellular Subpopulations Within the Tumor MicroenvironmentBioengineering (Basel)June 11, 2026Junya Peng, Lu Ping, Ruikang Dun, Lulu Liu, Yihong Shi, Ruizhe He, Qing Zhong, Yang Chen, Wenmin Tian, Yupei ZhaoProtein digestion under low-input conditions For proteolytic digestion, approximately 500 ng of Trypsin/Lys-C Mix (V5071, Promega, Madison, WI, USA) was added to each sample. Samples were incubated at 37 °C for 16–18 h with

(11) A proteogenomic RNA processing mechanism drives sex differences in meningiomaResearchSquareJune 2, 2026David Raleigh, Martha Cady, Ayush Aggarwal, Nicholas Stevers, Naomi Zakimi, Isabelle Liu, Kanish Mirchia, John Coukos, Pornparn Kongpracha, Yuan Zhou, et al.three times with 200μl acetonitrile. Rinsed beads were reconstituted in 10μL of 25mM Tryspin/Lys-C (V5071, Promega) in 50μM of ammonium bicarbonate. Samples were incubated at 37°C overnight. The following day,

(12) A comprehensive approach to elucidating the pathophysiology of kidney fibrosis based on extracellular vesicle proteomicsFrontiers in PhysiologyMay 29, 2026Yaerim Kim, Kyuhyeon Kim, Hong-Beom Park, Sunwha Lee, Mi-yeon Yu, Young Joo Kim, Soo Bin Choi, Woo Yeong Park, Kyubok Jin, Dong Ki Kim, et al.bicarbonate, protein digestion was performed overnight at 37 °C using trypsin/Lys-C Mix (Promega; Cat# V5071) at a 100:1 protein-to-protease ratio. All resulting peptides were acidified with 10% trifluoroacetic

(13) Decreased expression of Ly-1 antibody reactive clone (Lyar) triggers enhanced adipogenesis of bone marrow mesenchymal stromal cells in aged bone marrowPLoS OneMay 27, 2026Yu Shinyashiki, Yuta Onodera, Yusuke Kawashima, Natsumi Iwawaki, Toshiyuki Takehara, Koji Goto, Takeshi Teramura(S2 Table). On-bead digestion of IP samples for proteome analysis 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) were added to the IP-beads soaked in 50 mM ammonium bicarbonate, and then

(14) Cuproptosis causes meiotic metaphase I arrest by disrupting mitochondrial functions in oocytesCell Death DiscoveryMay 23, 2026You-Hui Lu, Can Wang, Lei-Ning Chen, Li-Tao Yi, Ke Xu, Xu-Feng Li, Shu-Chen Liu, Xiao-Yi Chen, Yi-Xiao Li, Qing-Yuan Sun, et al.iodoacetamide (Sigma, I1149). Protein digestion was carried out overnight with a Trypsin/Lys-C Mix (Promega, V5071). The resulting peptides were desalted on SOLAμ HRP SPE spin plates (Thermo Scientific, 60209-001),

(15) Adipocytes influence choroidal neovascularization via PRDM16EMBO Molecular MedicineMay 19, 2026Roberto Diaz-Marin, Masayuki Hata, Vera Guber, Vincent De Guire, Ariel M Wilson, Sergio Crespo-Garcia, Przemyslaw Sapiehaserum,Multicell,090450 Penicillin/Streptomycin,Corning,30-00-CI 3-isobutyl-1-methylxanthine,Sigma,I5879 dexamethasone,Sigma,D2915 insulin,Sigma,I9278 4-hydroxy-tamoxifen,Sigma,H7904 Matrigel,Corning,356230 EBM-2,Lonza,CC-3156 EGM-2 SingleQuots,Lonza,CC-4176 Hydroxyl PAC beads,ReSyn Biosciences,MR-HYX002 Lys‑C/Trypsin,Promega,V5071 Software,Software,Software MassHunter Quantitative analysis software,Agilent Technologies, ImageJ,U.

(16) Microfluidics-enabled proteomic profiling reveal iron-driven immune evasion by an antimicrobial-resistant pathogenCell Reports MethodsMay 18, 2026Chikim Nguyen, Chelsea Reitzel, Arjun Sukumaran, Kristina A. Ganzinger, Jennifer Geddes-McAlisterU5378 HEPES,Sigma-Aldrich,Cat H23830 acetone,Sigma-Aldrich,Cat 179124 trypsin/Lys-C,Promega,Cat V5071 acetonitrile,Sigma-Aldrich,Cat 34851 acetic acid,Sigma-Aldrich,Cat 5.33001 formic acid,Sigma-Aldrich,Cat

(17) Formation of Liver Metastases Is Accompanied by Accelerated Musculoskeletal Deficits in LLC Tumor HostsInt J Mol SciMay 15, 2026Paola Ortiz Gonzalez, Anna M. Miller, Luis F. Cardona Polo, Lilian I. Plotkin, Fabrizio Pin, Joshua R. Huotwas carried out with Trypsin/LysC Gold (0.5 µg, mass spectrometry grade, Promega Corporation Cat No: V5072, Madison, WI, USA) overnight at 35 °C. After digestion, samples were quenched with 0.4% trifluoroacetic

(18) Discovery of a paralog-selective p300 protein degrader with potent anti-cancer activity in hematological malignanciesNature CommunicationsMay 6, 2026Marwa S. Asem, Yan Zhai, Xiaohong Song, Milad Rouhimoghadam, Sreenivas Punna, Fritz G. Buchanan, Daniel T. Cohen, Ryan McClure, Stephanie Sandoval, Anlu Chen, et al.HEPES, pH 8.0, to lower the urea concentration to 2 M. Samples were digested with Trypsin/LysC (Promega, V5072) at a 1:50 enzyme-to-substrate ratio at 25 °C overnight with mixing (1350 rpm). The digestion was quenched

(19) Lipid Transfer Proteins and PI4KIIα Generate a Phosphoinositide-Linked ProteomeJournal of Biological ChemistryMay 5, 2026Noah D. Carrillo, Mo Chen, Poorwa Awasthi, Tianmu Wen, Xiangqin Chen, Giang Vu, Dhruv Brahmbhatt, Michael Herlihy, Benjamin B. Minkoff, Michael Sussman, et al.ammonium bicarbonate and proteolytically digested for 12 h at 37 °C using a 1:1 mixture of Trypsin:Lys-C (#V5071, Promega) and a protease:protein ratio of 1:50. Samples were acidified by addition of neat formic acid

(20) Characterization of KRASG12C inhibitor olomorasib single-agent and combination with activity in KRASG12C-mutant modelsNature CommunicationsMay 4, 2026Shengbin Peng, Youyan Zhang, Xi Lin, Chong Si, Robert Daniel Van Horn, Jack A. Dempsey, Eva Goetz, Robert J. Evans, Andrew Farber, Matthew J. Vandekopple, et al.Plate (Thermo Scientific, cat# 89807). Lysates were digested with 5 µg Trypsin/Lys-C Mix (Promega, cat# V5071) at 37 °C overnight. The digested samples were immunoprecipitated with the biotinylated anti-RAS peptide

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Trypsin/Lys-C Mix, Mass Spec Grade

Trypsin/Lys-C Mix, Mass Spec Grade from Promega

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Trypsin/Lys-C Mix, Mass Spec Grade from Promega

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