Fig 1: miR-9 overexpression in Area X downregulates the expression of FoxP1 and FoxP2 and disrupts dopamine signaling.(A) Expression of FoxP1 and FoxP2 mRNAs in Area X measured 4 weeks after injection with the lenti-miR-9 virus by qRT-PCR. p = 0.006, t(12) = 4.608 for FoxP1; p < 0.0001, t(12) = 7.062 for FoxP2, unpaired t-test. n = 7 for Area X; n = 4 for adjacent tissue. (B) Western blot showing expression of FoxP1 and FoxP2 proteins in Area X 4 weeks after injection with the lenti-miR-9 virus. p = 0.0007, t(6) = 6.313 for FoxP1; p = 0.0037, t(6) = 4.608 for FoxP2, unpaired t-test; n = 4. (C) Expression of dopamine receptors D1R and D2R mRNAs in Area X 4 weeks after injection with the lenti-miR-9 virus measured by qRT-PCR. For D1R, p < 0.0001, t(12) = 6.441; for D2R, p = 0.3384, t(12) = 0.9971, unpaired t-test; n = 7 for Area X. (D) DARPP-32 protein expression in Area X 4 weeks after injection with lenti-miR-9 virus. p = 0.0022, t(6) = 5.104, unpaired t-test; n = 4. Data are presented as mean ± SEM. Lenti-control and lenti-miR-9 viruses were injected into Area X of contralateral hemispheres, #40, #45, #49 and #52 in western blot images are IDs of animals.