Fig 1: Immunofluorescent detection of L-Type amino acid transporter 1 (LAT1) in wild type (WT) and LAT1 muscle-specific knockout (mKO) mouse extensor digitorum longus (EDL) muscle. Sections were stained with anti-LAT1 antibody (far left panels, green) and then co-stained with dystrophin (middle panels, red) for the identification of the sarcolemma. Representative images for both WT (A) and mKO (B) are shown. Scale bar in top left panel is 50 µm and all images were captured at the same magnification.
Fig 2: Immunofluorescent detection of LAT1 in human vastus lateralis muscle. Sections were stained with anti-LAT1 antibody (far left panels, green) and then co-stained with dystrophin (middle panels, red) for the identification of the sarcolemma. Representative images from two participants are displayed (A,B). Use of a peptide competition assay reduced the signal intensity of LAT1 staining (C). A strong positive signal was noted close to the sarcolemmal membrane and possible sites of blood vessels. In addition, a possible fibre type difference in LAT1 staining was noted. Immunoblots for the LAT1 protein displayed bands to be apparent in both giant sarcolemmal vesicles and cytosolic fractions before (PRE) and after (POST) an acute bout of resistance exercise (D) (n = 2). Scale bar in top left panel is 50 µm and all images were captured at the same magnification.
Fig 3: Immunofluorescent detection of LAT1 in human vastus lateralis muscle co-stained with myosin heavy chain 1 (MHC1) and dystrophin (DYS). Sections were stained with anti-LAT1 antibody (far left panels, green) and then co-stained with dystrophin (middle panels, red) for the identification of the sarcolemma and MHC1 (middle panels, red sarcoplasmic staining, marked with I) for the identification of type I muscle fibres. Representative images from two participants are displayed (A,B). Quantification of the mean immunofluorescent staining in type I and type II fibres displayed a greater staining in type II fibres (C). On average, 103 ± 17 fibres were quantified per participant. Values are Mean ± SE. *, significantly different to type I (p < 0.01). The scale bar in the top left panel is 50 µm and all images were captured at the same magnification.
Fig 4: Immunofluorescent detection of LAT1 in human vastus lateralis muscle co-stained with endothelial nitric oxide synthase (eNOS) and Wheat Germ Agglutinin (WGA). Sections were stained with anti-LAT1 antibody (green) and then co-stained with eNOS (red) for the identification of blood vessels. WGA (blue) was used to identify membrane borders. Representative images from two participants are displayed. Merged images display positive LAT1 staining localising close to positive eNOS staining, shown in greater detail in zoomed images (bottom panels). Scale bar in top left panel is 50 µm and all images (except zoomed images) were captured at the same magnification. Scale bar in bottom panels is 5 µm.
Supplier Page from Abcam for SLC7A5/LAT1 peptide