Mouse M-CSF, premium grade 130-101-704 from Miltenyi Biotec

(1) Targeting fibroblast derived thrombospondin 2 disrupts an immune-exclusionary environment at the tumor front in colorectal cancerNature CommunicationsNovember 23, 2025Kosuke Iwane, Yuki Nakanishi, Yu Muta, Jiayu Chen, Kento Yasumura, Mayuki Omatsu, Naoki Aoyama, Munehiro Ikeda, Yoko Masui, Liyang Cai, et al.RPMI-1640 supplemented with 10% FBS and macrophage colony-stimulating factor (M-CSF; Miltenyi Biotec, 130-101-704) for 5 days to differentiate into BMDM. BMDMs or DC2.4 cells were seeded at a density of 105/well in

(2) IRF7 drives macrophages to kill bacteria and improves septic outcomes via autophagyJCI InsightNovember 10, 2025Guiming Chen, Kangxin Li, Haihua Luo, Lianxu Zhao, Yong JiangU/mL penicillin, and 100 μg/mL streptomycin) in the presence of 20 ng/mL mouse M-CSF (Miltenyi Biotec, 130-101-704) for 7 days to generate macrophages (52). The cells were then treated with LPS (100 ng/mL) for 16 hours.

(3) Retinoblastoma-binding Protein 9 Suppresses Intestinal Inflammation and Inflammation-induced Tumorigenesis in MiceCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2025Kensuke Hamada, Yuki Nakanishi, Yu Muta, Mayuki Omatsu, Kosuke Iwane, Munehiro Ikeda, Jiayu Chen, Yoko Masui, Naoki Aoyama, Nobukazu Agatsuma, et al.penicillin-streptomycin. Cells were stimulated with macrophage colony-stimulating factor (M-CSF; Miltenyi Biotec, 130-101-704) for 5 days to differentiate into macrophages (BMDM). BMDM were seeded at a density of 1 × 106/well

(4) In vivo CAR T cell therapy against angioimmunoblastic T cell lymphomaJournal of experimental & clinical cancer research : CRSeptember 14, 2024Krug A, Saidane A, Martinello C, Fusil F, Michels A, Buchholz CJ, Ricci JE, Verhoeyen E.gentamycin (Thermofisher, 15,750–045) and murine M-CSF (macrophage colony stimulating factor; Miltenyi, 130–101-704). In parallel, T cells were pre-stimulated with IL-7 and IL-15 and transduced with mCD8-LVs at equivalent

(5) GSDMD is associated with survival in human breast cancer but does not impact anti-tumor immunity in a mouse breast cancer modelFrontiers in ImmunologyAugust 19, 2024Bart Boersma, Viola Puddinu, Arnaud Huard, Sébastien Fauteux-Daniel, Pratyaksha Wirapati, Sofia Guedri, Jean-Christophe Tille, Thomas McKee, Mikael Pittet, Gaby Palmer, et al.pyruvate (GIBCO, 11360070), 0.1% β-mercaptoethanol (GIBCO, 21985023), and M-CSF (10ng/ml, Miltenyi Biotec, 130-101-705). BMDM were cultured at a density of 80.000 cells/cm2 in a 37°C, 5% CO2 humidified environment. Medium

(6) Bone resorption by osteoclasts involves fine tuning of RHOA activity by its microtubule-associated exchange factor GEF-H1Frontiers in PhysiologyJanuary 19, 2024Anne Morel, Christophe Douat, Anne Blangy, Virginie Vivescalf serum (Biowest), 2 mM glutamine, 100 U/mL penicillin–streptomycin and 30 ng/ml M-CSF (Miltenyi, #130-101-703). Osteoclasts were then differentiated by culturing BMM in the same medium supplemented with 50 ng/mL

(7) Nanostructured Microparticles Repolarize Macrophages and Induce Cell Death in an In Vitro Model of Tumour-Associated MacrophagesPharmaceuticsJuly 5, 2023Salma Al-Fityan, Britta Diesel, Thorben Fischer, Emmanuel Ampofo, Annika Schomisch, Vida Mashayekhi, Marc Schneider, Alexandra K. Kiemercentrifuging. Obtained cells were cultured overnight (~16 h) in medium supplemented with mouse M-CSF (130-101-704, Miltenyi Biotec) in a 150-cm2 flask. The next day, non-adherent cells were collected and cultivated

(8) Bacterial Lipoproteins Shift Cellular Metabolism to Glycolysis in Macrophages Causing Bone ErosionMicrobiology spectrumJune 15, 2023Nguyen MT, Hu Z, Mohammad M, Schöttler H, Niemann S, Schultz M, Barczyk-Kahlert K, Jin T, Hayen H, Herrmann M.penicillin-streptomycin. We added 100 ng/mL macrophage colony-stimulating factor (M-CSF) (catalog no. 130-101-700; Miltenyi Biotec, Bergisch-Gladbach, Germany) on day 0 and day 3. The BMDMs were harvested after 7 days

(9) Statins and Bempedoic Acid: Different Actions of Cholesterol Inhibitors on Macrophage ActivationInternational journal of molecular sciencesNovember 19, 2021Linnenberger R, Hoppstädter J, Wrublewsky S, Ampofo E, Kiemer AK.(R)-Mevalonic acid lithium salt (#50838) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Murine M-CSF (#130-101-704), IFNγ (#130-105-782), IL4 (#130-094-061), and Il1β (#130-101-681) were obtained from Miltenyi Biotech

(10) TPC2 promotes choroidal angiogenesis and inflammation in a mouse model of neovascular age-related macular degenerationLife science allianceAugust 1, 2021Li Y, Schön C, Chen CC, Yang Z, Liegl R, Murenu E, Schworm B, Klugbauer N, Grimm C, Wahl-Schott C, et al.with 10% heat-inactivated FBS, 40 ng/ml murine monocyte-colony stimulating factor (M-CSF, Cat. no. 130-101-703; Miltenyi Biotec) and antibiotics for 7 d at 37°C and 5% CO2 to promote differentiation of cells into

(11) Conditional Gene Targeting Reveals Cell Type-Specific Roles of the Lysosomal Protease Cathepsin L in Mammary Tumor ProgressionCancersJuly 22, 2020Parigiani MA, Ketscher A, Timme S, Bronsert P, Schlimpert M, Kammerer B, Jacquel A, Chaintreuil P, Reinheckel T.an autoMACS™. The negative fraction containing purified monocytes was further cultured with CSF-1 (#130-101-704, Miltenyi)-supplemented medium and visualized using standard optics (20×/0.35 Ph1) equipped with an

(12) Altered glucocorticoid metabolism represents a feature of macroph-agingAging cellJune 1, 2020Valbuena Perez JV, Linnenberger R, Dembek A, Bruscoli S, Riccardi C, Schulz MH, Meyer MR, Kiemer AK, Hoppstädter J.(HVA, #H1252), and horseradish peroxidase (#P8250) were obtained from Sigma‐Aldrich. Murine M‐CSF (#130‐101‐704) and TNF‐α (#130–101–689) were obtained from Miltenyi Biotec. The 11β‐HSD1 inhibitor PF 915275 was obtained

(13) Selective agonist of TRPML2 reveals direct role in chemokine release from innate immune cellseLifeNovember 27, 2018Plesch E, Chen CC, Butz E, Scotto Rosato A, Krogsaeter EK, Yinan H, Bartel K, Keller M, Robaa D, Teupser D, et al.O111:B4,Sigma-Aldrich,Cat#L4391, "Fura-2, AM, cell permeant",ThermoFisher,Cat#F1201, "Mouse M-CSF, premium grade",Miltenyi Biotech,Cat#130-101-703, "Transferrin from human serum, Alexa FluorTM 546-conjugated",TermoFisher,Cat# T23364, "Transferrin

(14) Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in MacrophagesFrontiers in immunologyJanuary 1, 2018Hoppstädter J, Diesel B, Linnenberger R, Hachenthal N, Flamini S, Minet M, Leidinger P, Backes C, Grässer F, Meese E, et al.aurintricarboxylic acid (ATA, #A1895) were obtained from Sigma-Aldrich. Murine GM-CSF (#130-095-735), M-CSF (#130-101-704), IFN-γ (#130-105-782), IL-4 (#130-097-761), and TNF-α (#130–101–689) were obtained from Miltenyi Biotec.

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Mouse M-CSF, premium grade from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Mouse M-CSF, premium grade from Miltenyi Biotec

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