Description
Recombinant full-length human UBE2J1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: UBE2J1 is a member of the E2 ubiquitin-conjugating enzyme family which is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. UBE2J1 is play an important role in the homeostatic regulation of MHC class I assembly and expression (1). HRD1 and UBE2J1 associate in a complex together with non-beta2m bound MHC class I heavy chains, Derlin 1, and p97 and discriminate misfolded MHC class I from conformational MHC I-beta2m-peptide heterotrimers