Description
Recombinant human PDE7A (104-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE7A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE7A is a high-affinity cAMP-specific PDE and is expressed in T cell lines, peripheral blood T lymphocytes, epithelial cell lines, airway and vascular smooth muscle cells, lung fibroblasts, and eosinophils. PDE7 plays a critical role in the regulation of human T cell function and selective PDE7 inhibitors are being examined to treat immunological and inflammatory disorders (1). PDE7A plays an important role in the regulation of osteoblastic differentiation (2). PDE7A depletion by RNAi upregulates expression of several osteogenic genes and increases mineralization