Description
Recombinant full-length human CDK5P35 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: CDK5P35 is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5) that bind to cyclin to form active holoenzymes that play a pivotal role in the regulation of the eukaryotic cell cycle.The activation of CDK5 is required for proper development of the central nervous system. p35 is a regulatory subunit for CDK5 which is proteolytically cleaved by calpain and generating a p25 form that involved in the pathogenesis of cytoskeletal abnormalities and neuronal death in neurodegenerative diseases (1). Cdk/p35 plays a role in primary afferent nociceptive signaling and is required for cortical plate neurons to migrate past preexisting neurons and take up superficial positions to constitute the inside-outside layering order of cortical lamination(2)