Description
Recombinant human UBE2R2 (UBC3B) (2-end) was expressed in E. coli cells using an N-terminal His tag.
Scientific Background: UBE2R2 (UBC3B) or ubiquitin-conjugating enzyme E2R 2 encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP therby enhancing beta-catenin degradation. CK2-dependent phosphorylation of CDC34 and UBC3B functions by regulating BTRC substrate recognition (1). UBE2R2 complements a yeast cdc34 temperature-sensitive mutant. Deletion and site-directed mutagenesis demonstrated that CK2 phosphorylated UBE2R2 in the C-terminal domain at serine-233; mutagenesis of the corresponding serine residue at position 231 of human CDC34/UBC3 showed that this was the major CK2-phosphorylation site in this protein