Description
Recombinant full-length human PFTK1 (CDK14) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PFTK1, also known as PFTAIRE protein kinase 1, is a member of the CDC2 -related protein kinase family which is expressed primarily in the postnatal and adult nervous system (1). PFTK1 is highly expressed in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 interacts with 14-3-3-beta, 14-3-3-epsilon, 14-3-3-eta and 14-3-3-tau. Using PFTK1 mutant constructs and in vitro and in vivo binding studies, it was shown that PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms. (2). Significant upregulation of PFTK1 expression is observed in oesophageal squamous cell carcinoma (ESCC). PFTK1 is not only useful as a prognostic marker in ESCC, but also as a predictor of the response to chemotherapy