Protein A (HRP Conjugate) 12291S from Cell Signaling Technology

Product Specs
ItemProtein A (HRP Conjugate)
CompanyCell Signaling Technology
Price $184.00 Inquire
Catalog Number12291S
Size1 ml
ApplicationsW
Conjugate/TagHRP
Research AreaRelated Products
ReactivityAll

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Integrative biology shows DPP4 affects inflammatory response to eclampsia and cell model growth via p65/NLRP3/ASC/Caspase-1 pathwayFront GenetMarch 4, 2026Zhimin Bian, Li Hao, Rongjuan Yang, Jianghui SunUnited States), NLRP3 (Abcam, Catalog No. ab214185, UK), p-NLRP3 (Cell Signaling Technology, Catalog No. 12291, United States), p65 (Cell Signaling Technology, Catalog No. 8242, United States), and p-p65 (Cell Signaling Technology

(2) A guide to selecting high-performing antibodies for GCase (UniProt ID: P04062) for use in western blot, immunoprecipitation, and immunofluorescenceF1000ResearchDecember 15, 2025Donovan Worrall, Charles Alende, Maryam Fothouhi, Vera Ruíz Moleón, Sara González Bolívar, Riham Ayoubi, Vincent Francis, Carl Laflamme, Peter S. McPherson(H+L),62-6520,AB_2533947,polyclonal,1.5,0.75 Cell Signaling Technology,"Protein A, HRP conjugate",12291,NA,polyclonal,0.125,0.5 Thermo Fisher Scientific,Alexa Fluor™ 555-Goat anti-Rabbit IgG (H+L),A-21429,AB_2535850,polyclonal,2.0,0.5 Thermo Fisher Scientific

(3) A guide to selecting high-performing antibodies for Optineurin (UniProt ID: Q96CV9) for use in western blot, immunoprecipitation, and immunofluorescenceF1000ResearchOctober 17, 2025Vera Ruíz Moleón, Riham Ayoubi, Charles Alende, Maryam Fothouhi, Joel Ryan, Sara González Bolívar, Donovan Worrall, Thomas M Durcan, Claire M Brown, Vincent Francis, et al.(H+L),RGAM001,AB_3068333,recombinant polyclonal,1.0,0.5 Cell Signaling Technology,"Protein A, HRP conjugate",12291,NA,polyclonal,0.125,0.5 Millipore Sigma,Peroxidase-conjugated monoclonal anti-Flag M2,A8592,AB_439702,monoclonal,1.1,0.5 Millipore Sigma

(4) NDF/GLYR1 Promotes RNA Polymerase II Processivity via Pol II Binding and Nucleosome DestabilizationInt J Mol SciMay 19, 2025Ziwei Li, Jia Feiantibodies (as described above), the membrane was treated with HRP-conjugated Protein A (Cell Signaling 12291). For detecting biotinylated proteins, the membrane was directly incubated with HRP-conjugated streptavidin.

(5) A bacterial genotoxin reveals a p53-proteasome-LC3 regulatory axis that drives the suppression of autophagy in cells experiencing sublethal DNA damageiScienceApril 18, 2025D’Feau J. Lieu, Molly K. Crowder, Jordan R. Kryza, Batcha Tamilselvam, Paul J. Kaminski, Ik-Jung Kim, Ying-Xing Li, Eunji Jeong, Michidmaa Enkhbaatar, Henry Chen, et al.#4970, Cell Signaling Technology, USA), followed by HRP-linked protein A (1:10000 dilution in TBST; #12291, Cell Signaling Technology, USA). HRP-linked protein A is useful for preventing immunoprecipitation

(6) RNA-binding proteins and glycoRNAs form domains on the cell surface for cell-penetrating peptide entryCellApril 3, 2025Jonathan Perr, Andreas Langen, Karim Almahayni, Gianluca Nestola, Peiyuan Chai, Charlotta G. Lebedenko, Regan F. Volk, Diego Detrés, Reese M. Caldwell, Malte Spiekermann, et al.ProLong Diamond Antifade Mountant,Thermo Fischer Scientific,Cat# P36965 Protein A-HRP,Cell Signaling,Cat# 12291S Protein LoBind tubes,Eppendorf,Cat# 30108442 "Proteinase K, Molecular Biology Grade",New England Biolabs,Cat#

(7) A guide to selecting high-performing antibodies for STXBP1 (UniProt ID: P61764) for use in western blot, immunoprecipitation, and immunofluorescenceF1000ResearchDecember 24, 2024Riham Ayoubi, Charles Alende, Maryam Fotouhi, Sara González Bolívar, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF consortiumA-21432). Peroxidase-conjugated Protein A for IP detection is from Cell Signaling Technology, cat. number 12291. Antibody screening by western blot U-87 MG WT and STXBP1 KO cells were collected in RIPA buffer (25mM

(8) Improved elution strategy and new monoclonal anti-biotin antibody for LC-MS/MS characterization of protein biotinylation sitesBiochemistry and Biophysics ReportsJuly 1, 2024Yiying Zhupipeline. 2.3 Biotin labeling and spiked-in experiments Trypsin-digested mouse liver peptides (CST, #12291) were labeled with biotin by reacting with EZ-link NHS-biotin. First, 1 mg of a lyophilized mouse liver

(9) Glycosylation Modulation Dictates Trafficking and Interaction of SARS-CoV-2 S1 Subunit and ACE2 in Intestinal Epithelial Caco-2 CellsBiomoleculesApril 30, 2024Marianne El Khoury, Dalanda Wanes, Maura Lynch-Miller, Abdullah Hoter, Hassan Y. Naimanti-ACE2 antibody [EPR4435(2)],0.231,1:5000,"Abcam (Cambridge, UK)",ab108252 Protein A (HRP Conjugate),-,1:1000,"Cell Signaling (Danvers, MA, USA)",12291 Goat anti-mouse IgG (H+L) Secondary antibody HRP,0.4,1:5000,"Thermo Fisher Scientific (Waltham, MA,

(10) Lipid rafts disruption by statins negatively impacts the interaction between SARS-CoV-2 S1 subunit and ACE2 in intestinal epithelial cellsFrontiers in MicrobiologyJanuary 8, 2024Marianne El Khoury, Hassan Y. Naimμg/μL,"1:5,000",Santa Cruz Biotechnology,sc-28320 Protein A (HRP Conjugate),-,"1:1,000",Cell Signaling,12291 Actin,2 μg/μL,"1:5,000",Santa Cruz Biotechnology,sc-47778 mAb HBB 3/775/42 (DPPIV),-,"1:5,000",Hauri

(11) A guide to selecting high-performing antibodies for ADNP (UniProt ID: Q9H2P0) for use in western blot, immunoprecipitation, and immunofluorescenceF1000ResearchJanuary 1, 2024Ruíz Moleón V, Alende C, Fotouhi M, Ayoubi R, González Bolívar S, Laflamme C, NeuroSGC/YCharOS/EDDU collaborative group, ABIF consortium.A-21429. Peroxidase-conjugated Protein A for IP detection is from Cell Signaling Technology, cat. number 12291. Antibody screening by western blot DMS 53 ctrl and ADNP KD cells were collected in RIPA buffer (25mM

(12) Biochemical Characterization of SARS-CoV-2 Spike RBD Mutations and Their Impact on ACE2 Receptor BindingFrontiers in molecular biosciencesJanuary 1, 2022Hoter A, Naim HY.peroxidase-conjugated protein A (protein A-HRP) was purchased from Cell Signaling Technology (cat. #12291S), and mouse anti-FLAG was from Sigma-Aldrich Chemie GmbH (Munich, Germany). Anti-β-actin (C4) was bought

(13) BCR-Induced Ca²⁺ Signals Dynamically Tune Survival, Metabolic Reprogramming, and Proliferation of Naive B CellsCell reportsApril 14, 2020Berry CT, Liu X, Myles A, Nandi S, Chen YH, Hershberg U, Brodsky IE, Cancro MP, Lengner CJ, May MJ, et al.RRID:AB_312951 Purified anti-mouse CD16/32 Antibody,BioLegend,Cat#101302 RRID:AB_312801 Protein A HRP,Cell Signaling,Cat#12291 "Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP",Thermo Fisher,Cat#65–6120 RRID:AB_2533967 "Goat

(14) AKT2 is the predominant AKT isoform expressed in human skeletal musclePhysiological reportsMarch 1, 2018Matheny RW Jr, Geddis AV, Abdalla MN, Leandry LA, Ford M, McClung HL, Pasiakos SM.(C67E7) (rabbit monoclonal; #4691), AKT (Pan) (40D4) (mouse monoclonal; #2920), and Protein A HRP (#12291S), were purchased from Cell Signaling Technologies (Danvers, MA). Normal rabbit IgG (#sc‐2027) and normal mouse IgG

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Protein A (HRP Conjugate) from Cell Signaling Technology

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Protein A (HRP Conjugate) from Cell Signaling Technology

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