Fig 1: Proposed model for avß3-mediated potentiation of TH2 cell responses.Naïve CD4+ T cells express low levels of integrin avß3 which contribute to T cell activation and signalling via the IL-2/CD25/STAT5 axis. IL-4-mediated Gata3 induction upregulates avß3 during TH2 cell differentiation, permitting intercellular interactions among TH2 cells via avß3-Thy1 binding. Such interactions enhance mTOR signalling and support optimal TH2 responses in vivo.
Fig 2: Thy-1-avß3 interaction promotes TH2 cell differentiation in vitro.(a) Flow cytometric analysis of cytokine expression by TH2 cells cultured with DMSO or cilengitide. Data are representative of 3 experiments with 4 biologically independent samples in each experiment; mean ± SD; unpaired two-sided t-test; *P=0.0152, ***P=0.0002 (IL-13 MFI) and 0.0010 (IL-5 MFI). (b) Flow cytometric analysis of cytokine expression by TH2 cells cultured with isotype or anti-av antibody; **P=0.0050 (% IL-13+ cells), 0.0043 (% IL-5+ cells), 0.0017 (IL-13 MFI), 0.0089 (IL-5 MFI). (c) Flow cytometric analysis of cytokine expression by TH2 cells cultured with isotype or anti-ß3 antibody; ****P<0.0001. (b) & (c) Data are representative of 2 experiments with 5 biologically independent samples in each experiment; mean ± SD; unpaired two-sided t-test. (d) Regularised log expression of avß3 ligands by TH cells. Data from Th-Express. (e) Flow cytometric analysis of Thy1 expression by TH cells. Data are representative of 2 experiments with 4 biologically independent samples in each experiment; mean ± SD; one-way ANOVA with Tukey’s post-hoc test; ***P=0.0005. (f) Detection of Thy1 protein (25 kDa) in immunocomplexes generated with TH2 cell lysate coimmunoprecipitated with anti-av or anti-ß3 antibodies. Data are representative of 2 experiments. (g) Quantification of cell clusters and percentage clustered area of anti-CD3 and anti-CD28 stimulated TH cells cultured with isotype or anti-Thy1 antibody. Data are representative of 2 experiments with 4 biologically independent samples in each experiment; paired two-sided t-test; *P=0.0387. (h) Flow cytometric analysis of cytokine expression by TH2 cells cultured in the presence of isotype or anti-Thy1 antibody. Data are representative of 2 experiments with 5 biologically independent samples in each experiment; paired two-sided t-test; *P=0.0145, **P=0.0081 (% IL-5+ cells), 0.0020 (IL-13 MFI). (i) Flow cytometric analysis of cytokine expression by TH2 cells cultured in the presence of Fc-of Thy1-Fc-conjugated beads, and additionally with either isotype or anti-ß3 antibody; **P=0.0013. (j) Flow cytometric analysis of cytokine expression by Cd4CreERT2 or ItgaviCD4KO TH2 cells cultured in the presence of Fc-of Thy1-Fc-conjugated beads; *P=0.0201. (i) – (j) Data are representative of 2 experiments with 4 biologically independent samples in each experiment; unpaired two-sided t-test.
Supplier Page from R&D Systems, a Bio-Techne Brand for CD90/Thy1 Fc Chimera Protein
Available conjugates: Sizes Available: 50 ug