Micrococcal Nuclease 10011S from Cell Signaling Technology

Product Specs
ItemMicrococcal Nuclease
CompanyCell Signaling Technology
Price $80.00 Inquire
Catalog Number10011S
Size60 µl
ApplicationsChIP
Molecule NameMicrococcal Nuclease
Research AreaRelated Products

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Cell Signaling Technology

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(1) ChREBP-β Exacerbates Renal Tubular Disorders Caused by Fructose via ATF4FASEB JApril 30, 2026Ting Fang, Xinyu Yang, Xiaoqing Deng, Jingyi Wang, Ting Li, Hang Guo, Bei Sun, Liming Chentemperature, and the reaction stopped using 0.5 M glycine. After digesting with micrococcal nuclease (#10011, Cell Signaling Technology) and sonication, the chromatin was incubated with an anti‐ChREBP antibody

(2) Mathematical modelling of ribosome competition informs testable treatment strategies for drug-tolerant cancer persister cellsiScienceApril 17, 2026Xinpu Tang, Yuqing Wang, Yi Pu, Kaixiu Li, Zheyu Ding, Mengyao Wang, Luis Almeida, Michael Cerezo, Yarong Cao, Caroline Robert, et al.dounced and homogenized. Chromatin was digested with Micrococcal Nuclease (Cell Signaling Technology, #10011) at 37°C for 20 minutes, stopped with 0.5M EDTA. Chromatin was resuspended in hypotonic buffer (0.2mM

(3) Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasisCell Oncol (Dordr)April 1, 2026Chang Liu, Qianru Yu, Difei Wang, Zheng Duan, Xin Zhang, Jiao Wang, Xiaoyu Qi, Jiayin Ye, Qian Zhao, Jianrong He, et al.at RT. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 µl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. Keeping 10% of the sample as input DNA,

(4) GPR3 is an immediate-early gene-like GPCR regulating CREB-dependent neuronal differentiationiScienceMarch 20, 2026Shigeru Tanaka, Fumiaki Ikawa, Hiroko Shiraki, Kana Harada, Izumi Hide, Norio SakaiChromatin digestion was carried out enzymatically using Micrococcal Nuclease (Cell Signaling Technology, #10011). The nuclease volume was optimized (0.5 μL per IP) to generate chromatin fragments of approximately

(5) Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLDNature CommunicationsMarch 13, 2026Xiaojia Huang, Sen Liang, Nan Zhao, Jingjing Ding, Siyi Jiang, Changpei Gan, Huayu Zhang, Lei Zuo, Yangyang Li, Xiaoxun Zhang, et al.and then were scraped into Eppendorf tubes. Next, 1 µl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. Subsequently, the digested

(6) Disrupted O-GalNAc glycosylation as a mechanism and biomarker of SLC35A2 -associated epilepsybioRxivMarch 4, 2026Robert G. Mealer, James J. Anderson, Sheridan L. Smith, Brianna M. Masters, Samuel H. Barth, Kaitlyn D. J. Huizar, Sahibjot Sran, Hyojung Yoon, Amanda Ringland, Savannah J. Muron, et al.nitrocellulose blots with the following FITC lectins (Vector Labs, VVA #FL-1231–2, PNA #FL-1071–5, ConA #FL-10011–5, AAL #FL-1391–1, and RCA #FL1081–1) at 25 mg/mL, biotinylated WFA (#B-1355–2) at 25 mg/mL, or primary

(7) Chromatin remodeler BAF maintains HBV cccDNA transcriptional competence and represents a therapeutic targetNucleic Acids ResFebruary 10, 2026Dan Huang, Yi Zheng, Enze Deng, Xinlei Ji, Yecheng Zhang, Hao Sun, Yingshan Chen, Yongxuan Yao, Yuan Zhou, Mingxia Zhang, et al.chromatin was digested with 0.5 μl micrococcal nuclease for 20 min at 37°C (Cell Signaling Technology, #10011), followed by sonication (Scientz JY88-IID sonicator, 95% power, 9.9 s on/2 s off, 10 cycles). The supernatants

(8) ZFTA–RELA ependymomas make itaconate to epigenetically drive fusion expressionNatureFebruary 4, 2026Siva Kumar Natarajan, Joanna Lum, James Haggerty Skeans, Minal Nenwani, Sanjana Eyunni, Mateus Mota, Jill M. Bayliss, Akash Deogharkar, Erin Taya Hamanishi, Matthew Pun, et al.was achieved by sonication (QSonica, 800R) and by micrococcal nuclease (Cell Signaling Technologies, 10011) digestion to a final size of 150–300 bp. ChIP was performed using a Simple Chip Enzymatic Chromatin

(9) DNA damage induced by HIV-1 Vpr triggers epigenetic remodeling and transcriptional programs to enhance virus transcription and latency reactivationPLoS BiolFebruary 2, 2026Nicholas Saladino, Emily Leavitt, Hoi Tong Wong, Jae-Hoon Ji, Diako Ebrahimi, Daniel J. Salamangotwice. To fragment genomic DNA, nuclei were treated with micrococcal nuclease (MNase; Cell Signaling 10011) and incubated for 30 min at 37 ℃ with frequent mixing after which this reaction was stopped using 0.2%

(10) Multi-Omics Analysis of Human Blood Cells Reveals Unique Features of Age-Associated Type 2 CD8 Memory T CellsAging CellFebruary 1, 2026Hiroyuki Matsui, Marlene Cervantes, Mir M. Khalid, Alan Tomusiak, Varun Dwaraka, Jorge Landgrave-Gomez, Prasanna Vadhana Ashok Kumaar, Qingwen Chen, Jessica Lasky-Su, Jake Stone, et al.cells were incubated and lysed in successive steps with Buffers A & B (CST#14282). Diluted MNase (CST#10011) was added to fragment the DNA, digestion was stopped using EDTA before collecting DNA in the supernatant.

(11) Long-term memory in epithelia: transient IFNγ exposure drives stable repression of TFF1 in gastric epithelial cells via epigenetic changesFrontiers in ImmunologyJanuary 7, 2026Antonia Voli, Daniela Eletto, Fatima Maria Mentucci, Chiara Centrella, Martina Pannetta, Francesco Boccellato, Alfonso Finizio, Silvana Morello, Caterina Giraulo, Amalia Porta, et al.(Cell Signaling, 7005S, USA) for neutralization. Samples were treated with Micrococcal Nuclease (Cell Signaling, 10011, USA) for 20 min at 37°C to obtain DNA fragments of approximately 150–900 bp. Nuclei were sonicated

(12) Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancerFEBS LettersJanuary 1, 2026Diego Claro de Mello, Marcella Maringolo Cristovão, Guilherme Henrique, Vinicius Gonçalves Rodrigues, Caroline Serrano‐Nascimento, Edna Teruko Kimura, Cesar Seigi Fuziwaratubes. For the input sample, chromatin digestion was performed using the Micrococcal Nuclease protocol (#10011, Cell Signaling), as previously described, followed by sonication with three 20‐s pulses at 40% amplitude

(13) A positive feedback loop between TNS1 and ZEB1 promotes TGFβ-induced epithelial-to-mesenchymal transition in lung cancerCommunications BiologyNovember 26, 2025Tingting Zhang, Zhang Li, Yue Ming, Jiao Li, Zixia Ye, Dongdong Luo, Rui Wang, Yang Yang, Xiaojuan Yang, Yong Pengchromatin fragmented by enzymatic digestion with 0.5 μL Micrococcal Nuclease (Cell Signaling Technology, #10011) at 37 °C for 20 min, followed by sonication. Fragmented chromatin was immunoprecipitated overnight

(14) EWS::FLI1-DHX9 interaction promotes Ewing sarcoma sensitivity to DNA topoisomerase 1 poisons by altering R-loop metabolismOncogeneOctober 7, 2025Joaquin Olmedo-Pelayo, Esperanza Granado-Calle, Daniel Delgado-Bellido, Laura Lobo-Selma, Carmen Jordan-Perez, Ana T. Monteiro-Amaral, Anna C. Ehlers, Shunya Ohmura, Daniel J. Garcia-Dominguez, Carlos Mackintosh, et al.For chromatin fragmentation, extracts were incubated with 2 µl Micrococcal Nuclease (Cell Signaling, 10011) and sonicated in a Bioruptor (Diagenode) for 2 cycles at high intensity. Extracts were incubated with

(15) CREB Regulates Cisplatin Resistance by Targeting TNKS and KDM6A in NSCLC cell-Derived Tumor SpheroidInt J Biol SciJuly 28, 2025Ji Hae Lee, Youngjoo Kwon, Kyungsil Yoong for 5 min at 4°C and then resuspended in buffer B (#7007, Cell Signaling). Micrococcal Nuclease (#10011, Cell Signaling) was added at 2.5 μl per 2 × 107 cells and incubated 37°C for 20 min to digest chromatin.

(16) mTORC1-Dependent Regulation of the CCL24-CCR3 Axis Controls Granuloma Formation and Maintenance in SarcoidosisbioRxivJuly 16, 2025Xiongjian Rao, Jinpeng Liu, Derek B Allison, Douglas A Harrison, Ka Wing Fong, Yuanyuan Wu, Daheng He, Jia Peng, Zhiguo Li, Chi Wang, et al.then quenched with 0.125 M glycine for 5 min. Nuclei was digested by micrococcal nuclease (CST, Cat#10011), and the supernatant was used for immunoprecipitation with the indicated antibody. The primers which

(17) PPARγ controls ESCRT-dependent fibroblast-like synoviocyte exosome biogenesis and alleviates chondrocyte osteoarthritis mediated by exosomal ANXA1Journal of Orthopaedic TranslationJuly 1, 2025Shuangshuo Jia, Yue Yang, Jiabao Liu, Ziyuan Wang, Lunhao Baibetween DNA and protein. Fragmented chromatin was then extracted using 0.5 μL of micrococcal nuclease (10011; CST) and ChIP buffer. These were incubated overnight with rabbit IgG (2729; CST) as a control or anti-PPARγ

(18) The Chromatin Remodeler Protein CHD4 Cooperates With NKX2.2 to Regulate Pancreatic Beta Cell IntegritybioRxivJune 20, 2025Dylan K. Sarbaugh, Thais Gaia Oliveira, Michelle A. Guney, Victoria M. Hoelscher, Christopher J. Hill, Cole R. Michel, Kristen L. Wells, Richard K.P. Benninger, Lori Susselchromatin was fragmented using a Diagenode Bioruptor Pico sonicator and Micrococcal Nuclease (CST, Cat. # 10011) to fragment chromatin to approximately 150-900bp. 2% of samples was removed to be used as input before

(19) Ubinuclein 2 is essential for mouse development and functions in X chromosome inactivationPLoS GenetJune 2, 2025Asun Monfort, Giulio Di Minin, Sarah Sting, Charles Etienne Dumeau, Peter Scambler, Anton WutzChromatin was digested using 60 μl of LB containing 0.3 μl Micrococcal nuclease (Cell signalling, #10011S) at 37oC for 15 min. Reactions were stopped with 15 μl 0.5 M EDTA, and Stop Buffer (SB) (50 mM TrisHCl

(20) STAT3, MYC, and EBNA1 cooperate through a ZC3H18 transcriptional network to regulate survival and proliferation of EBV-positive lymphomasPLoS PathogMay 12, 2025Huanzhou Xu, Siva Koganti, Chenglong Li, Michael T. McIntosh, Sumita Bhaduri-McIntoshSignaling) for 2 minutes. Lysates were resuspended in 150 µL Buffer B with 0.5 µL Micrococcal Nuclease (10011, Cell Signaling) and incubated at 37°C for 20 minutes. After centrifugation to remove the supernatant,

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Micrococcal Nuclease from Cell Signaling Technology

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