PE Streptavidin (High Concentration) 405245 from BioLegend

Product Specs
ItemPE Streptavidin (High Concentration)
CompanyBioLegend
Price $238.00Supplier Page View Company Product Page
Catalog Number405245
Size500 µg
ApplicationsFC - Quality tested, ICFC - Verified
Concentration1.0 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Conjugate/TagPE
SpeciesHuman, Mouse, Rat, All Species
TargetBiotin

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Virus glycoprotein nanodisc platform for vaccine analyticsNature CommunicationsFebruary 10, 2026Kimmo Rantalainen, Alessia Liguori, Gabriel Ozorowski, Claudia Flynn, Jon M. Steichen, Olivia M. Swanson, Patrick J. Madden, Sabyasachi Baboo, Swastik Phulera, Anant Gharpure, et al.Alexa Fluor 647 Streptavidin (BioLegend #405237), BV421 Streptavidin (BioLegend #405225), PE Streptavidin (BioLegend #405245), PE-Cy7 Streptavidin (BioLegend #405206), BV711 Streptavidin (BioLegend #405241), BUV615 Streptavidin

(2) Comparative analysis of cellular immune responses to four seasonal inactivated influenza vaccines in younger and older adultsThe Journal of ImmunologyFebruary 9, 2026Vanessa Silva-Moraes, Laise Rodrigues Reis, Ted M Ross1 h at 4 °C at a 1:2.6 mass ratio (eg, 60 ng HA with 156 ng SA). HA (H1N1) was labeled with SA-PE (#405245, Biolegend, San Diego, California, USA), and HA (H3N2) was labeled with SA-APC (#405243, Biolegend,

(3) Integrated analysis of humoral and memory B-cell responses reveals distinct immune landscapes shaped by influenza vaccine platformsnpj VaccinesDecember 23, 2025Laise Rodrigues Reis, Ted M. Rossmultimerization with fluorochrome-conjugated streptavidin (SA): A/Victoria/4897/2022 (H1N1) conjugated to SA-PE (#405245, Biolegend, San Diego, CA, USA), A/Thailand/8/2022 (H3N2) conjugated to SA-APC (#405243, Biolegend,

(4) Resistance potential of the HLA-A2-restricted immunodominant SARS-CoV-2-specific CD8+ T cell receptor repertoire to antigenic driftNature CommunicationsDecember 18, 2025Michael J. Malone, Chao Huang, Yong Zhang, Yi Qi, Lea Williams, Laura F. Su, Jizhong Lou, Ning Jiang(S-1002, TriLink Biotechnologies) was used during the modification of PE- or APC-labeled streptavidin (405245 or 405243, BioLegend). The barcode construct used was: 5’- ATGGACGACGACGACAAG [10 N] [20NT_BARCODE]

(5) Highly functional and prolonged germinal center T follicular helper cell responses are associated with enhanced neutralizing antibody developmentImmunityDecember 9, 2025Ester Marina-Zárate, Henry J. Sutton, Paul G. Lopez, Tasha K. Altheide, Michael Bick, Iszac Burton, Elana Ben-Akiva, Katarzyna Kaczmarek Michaels, Kesha Hyacinth, Brandon S. Healy, et al.Streptavidin,Invitrogen,Cat# S32357 BV421 Streptaidin,BioLegend,Cat# 405225 PE Streptavidin,BioLegend,Cat# 405245 7AAD,Thermo Fisher Scientific,Cat# 00-6993-50 LIVE/DEAD Fixable Blue,Invitrogen,Cat# L23105 Fixable

(6) Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemiaNature Biomedical EngineeringJuly 1, 2025Chao Ma, Huishu Wang, Lunan Liu, Ruiqi Chen, Nandana Mukherjee, Jie Tong, Shadab Kazmi, Xiangyi Fang, Matthew T. Witkowski, Iannis Aifantis, et al.(1:50, catalogue number FM3-BY54, ACRO Biosystems), followed by incubation with PE (catalogue number 405245, BioLegend) or APC (catalogue number 405243, BioLegend) conjugated streptavidin (1:50), or directly

(7) Coordinated early immune response in the lungs is required for effective control of SARS-CoV-2 replicationNat CommunJune 25, 2025Klara Lenart, Hendrik Feuerstein, Mariana Prado Marmorato, Laura Perez Vidakovics, Gerald McInerney, Mimi Guebre-Xabier, Jessica F. Trost, Bengt Eriksson, Gale Smith, Nita Patel, et al.proteins to streptavidin (SA)-fluorophore complexes (SA-APC, SA-PE and SA-BV421, all Biolegend, #405243, #405245, and #405225). PBMC samples were stained with 100 ng of respective protein probes for 20 min at 4 °C,

(8) Virus glycoprotein nanodisc platform for vaccine designbioRxivJune 19, 2025Kimmo Rantalainen, Alessia Liguori, Gabriel Ozorowski, Claudia Flynn, Jon M. Steichen, Olivia Swanson, Patrick J. Madden, Sabyasachi Baboo, Swastik Phulera, Anant Gharpure, et al.Alexa Fluor 647 Streptavidin (BioLegend #405237), BV421 Streptavidin (BioLegend #405225), PE Streptavidin (BioLegend #405245), PE-Cy7 Streptavidin (BioLegend #405206), BV711 Streptavidin (BioLegend #405241), BUV615 Streptavidin

(9) Diverse priming outcomes under conditions of very rare precursor B cellsImmunityApril 8, 2025Patrick J. Madden, Ester Marina-Zárate, Kristen A. Rodrigues, Jon M. Steichen, Monolina Shil, Kaiyuan Ni, Katarzyna Kaczmarek Michaels, Laura Maiorino, Amit A. Upadhyay, Swati Saha, et al.Streptavidin,-,BioLegend (405237),- BV421 Streptavidin,-,BioLegend (405225),- PE Streptavidin,-,BioLegend (405245),- Viability APC-eFluor506,-,Thermo Fisher Scientific (65-0866-18),1:500 CD8a APC-eFluor780,RPA-T8,BD Biosciences

(10) Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokinesNature communicationsFebruary 26, 2025Parween F, Singh SP, Kathuria N, Zhang HH, Ashida S, Otaizo-Carrasquero FA, Shamsaddini A, Gardina PJ, Ganesan S, Kabat J, et al.washing with FACS buffer, the cells were stained by incubating with 1 μg/mL of streptavidin-PE (Cat# 405245, Biolegend) for 45 min at 4 °C. Samples were analyzed using an LSRFortessa flow cytometer (BD Biosciences),

(11) MAIT cells protect against sterile lung injuryCell ReportsFebruary 25, 2025Xiawei 夏维 Zhang 张, Shuailin 帅霖 Li 李, Wojciech Lason, Maria Greco, Paul Klenerman, Timothy S.C. HinksViolet 421 (BV421)-Streptavidin and Phycoerythrin (PE)-Streptavidin (BioLegend, Cat. No. 405225 and 405245, respectively) following the NIH Tetramer Facility’s guidelines. Antibodies staining for flow cytometry

(12) Monocyte uptake of polymeric peptidoglycan is bimodal and governed by complement C3 and C4 opsoninsJCI InsightJanuary 23, 2025Narcis I. Popescu, Jędrzej Kluza, Megan A. Reidy, Elizabeth Duggan, John D. Lambris, Linda F. Thompson, K. Mark Coggeshallagainst CD18) or BioLegend (clones m24 against CD18 and HIB19 against CD19, PE-labeled [catalog no. 405245] and BV421-labeled [catalog no. 405225] streptavidins, and Zombie Violet [catalog no. 423114] or Aqua

(13) Multiplexed Antibody Sequencing and Profiling of the Human Hemagglutinin-specific Memory B Cell Response following Influenza VaccinationThe Journal of ImmunologyDecember 1, 2024Nagarajan Raju, Kevin J. Kramer, Mattia Cavallaro, Roberta A. Diotti, Andrea R. Shiakolas, Yailin Campos Campos Mota, Robert A. Richardson, Ileia J. Scheibe, Ted M. Ross, Ivelin S. Georgiev, et al.concentration (1 mg/mL) phycoerythrin (PE)- or allophycocyanin (APC)-conjugated streptavidin (cat. n. 405245 and 405243, respectively, BioLegend, San Diego, CA, USA) to a final 8-fold molar excess, in ice-cold

(14) Geometric parameters that affect the behavior of logic-gated CAR T cellsFrontiers in ImmunologyJanuary 26, 2024Alexander C. Partin, Richele Bruno, Sanam Shafaattalab, Erica Vander Mause, Aaron Winters, Mark Daris, Casey Gahrs, Claudia A. Jette, Breanna DiAndreth, Mark L. Sandberg, et al.mesothelin (296-580) protein, His, Avitag (Acro Biosystems, Cat#MSN-H82E9); PE Streptavidin (BioLegend, Cat# 405245). Results Section 1: height of the B-antigen influences blocking CAR-T platforms have generally

(15) LRRC15 mediates an accessory interaction with the SARS-CoV-2 spike proteinPLoS biologyFebruary 1, 2023Shilts J, Crozier TWM, Teixeira-Silva A, Gabaev I, Gerber PP, Greenwood EJD, Watson SJ, Ortmann BM, Gawden-Bone CM, Pauzaite T, et al.To each well of the dilution series, 1.5 pmol of fluorescently conjugated streptavidin (Biolegend #405245 or Biolegend #405237) was added and allowed to incubate for at least 1 hour. Samples were then transferred

(16) Vaccine subtype and dose interval determine immunogenicity of primary series COVID-19 vaccines in older peopleCell reports. MedicineSeptember 20, 2022Parry H, Bruton R, Ayodele R, Sylla P, McIlroy G, Logan N, Scott S, Nicol S, Verma K, Stephens C, et al.anti-CD19,Biolegend,Cat # 302242;RRID:AB_2561668 Live dye,Thermo Fisher,Cat # L34955 PE Streptavidin,Biolegend,Cat # 405245 APC Streptavidin,Biolegend,Cat # 405207 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, SARS-CoV-2

(17) A physical wiring diagram for the human immune systemNatureAugust 1, 2022Shilts J, Severin Y, Galaway F, Müller-Sienerth N, Chong ZS, Pritchard S, Teichmann S, Vento-Tormo R, Snijder B, Wright GJ.biotinylated recombinant protein complexed with R-phycoerythrin-conjugated streptavidin (BioLegend 405245). Typically, a series of four tetramer quantities were used, ranging from 30 pmol to 1 pmol in a volume

(18) Tetramer-Associated T Cell Receptor SequencingMethods in molecular biology (Clifton, N.J.)January 1, 2022Malone M, Ma KY, Zhang SQ, Jiang N.

(19) A proteome-wide genetic investigation identifies several SARS-CoV-2-exploited host targets of clinical relevanceeLifeAugust 17, 2021Anisul M, Shilts J, Schwartzentruber J, Hayhurst J, Buniello A, Shaikho Elhaj Mohammed E, Zheng J, Holmes M, Ochoa D, Carmona M, et al.protein biotinylation "Peptide, recombinant protein",Streptavidin R-phycoerythrin,BioLegend,Cat.# 405245,For tetramer staining in cell-based binding assay "Chemical compound, drug","DAPI (4',6-diamidino-2-phenylindole)",BioLegend,Cat.#

(20) The discriminatory power of the T cell receptoreLifeMay 25, 2021Pettmann J, Huhn A, Abu Shah E, Kutuzov MA, Wilson DB, Dustin ML, Davis SJ, van der Merwe PA, Dushek O.algorithm software, algorithm other other Retronectin Takara Bio T100B Streptavidin-PE Biolegend 405245 biotinylated BSA Thermo Fisher Scienti c 29130 Streptavidin Thermo Fisher Scienti c 434301 Peptide

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PE Streptavidin (High Concentration) from BioLegend

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PE Streptavidin (High Concentration) from BioLegend

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