Description
r-hCRP is produced into culture medium by DNA recombinant procedure in E. Coli as secretory protein. By expressing kil gene, the outer membrane of E. Coli becomes permeable, allowing r-hCRP to be excreted without cell lysis. r-hCRP has similar biological property in terms of Ca2+-binding ability to the native protein, and is purified by affinity chromatography. N-terminal amino acid sequence of r-hCRP is the same as that of the native human CRP