Fig 1: Spatial distribution analysis of hepatocyte genes in response to NGC or N10 dengue virus. (A) Spatial location of clusters in the uninfected group, NGC group, and N10 group. (B–I) Spatial expression distribution and expression quantity of candidate genes in the uninfected, NGC, and N10 groups. Candidate genes include Nrg4 (B,C), Sult1e1 (D,E), Ddit4 (F,G), Cxcl13 (H,I).
Fig 2: N10 dengue virus infection triggers change in Nrg4. (A) An overview of molecular validation (B–E). The relative mRNA expression levels of Cxcl13 (B), Ddit4 (C), Sult1e1 (D), and Nrg4 (E) in the liver tissues (n = 10). (F–I) The relative protein expression levels of SULT1E1 (F,I) and NRG4 (G,H) in the liver tissues (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
Fig 3: Pseudotime analysis of hepatocyte genes in response to NGC or N10 dengue virus. (A) UMAP of hepatocytes by differentiation state inferred using CytoTRACE. (B) Pseudotime trajectory projected onto the UMAP of hepatocytes. (C,D) Pseudotime analysis of the uninfected, NGC-infected, and N10-infected hepatocytes. The trajectory spans uninfected (arrow) to NGC-infected to N10-infected hepatocytes. (E,F) Heatmaps showing different gene expression patterns during the differentiation of node 1 and node 2 over pseudotime. (G,H) The gene expression distribution of Nrg4 over pseudotime.
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