Fig 1: Large-scale purification of CD44. (A) Construct design using pExpreS2-1-C3-10H-SIII. (B) CD44 is pure and monodisperses after SEC. (C) Binding assay confirms that S2-derived CD44 binds to its ligand hyaluronic acid (HA) with similar affinity as E. coli– or mammalian-derived CD44. Data are presented from a single biological replicate and were fit via four component (variable slope) nonlinear regression using GraphPad Prism.
Fig 2: Spectra of BR sensors functionalized for CD44 detection, for different concentrations ranging from 1 fM to 100 nM. (a) First sensor; (b) second sensor.
Fig 3: Detection of cancer biomarkers with BR sensors, using a simultaneous four-sensor network. Each chart reports the experimental data, sampling the concentrations from 1 fM to 100 nM with a 10× increment; error bars report ± standard deviation of 6 consecutive measurements, acquired after 10 min stabilization and sampled at each 2 min interval. Solid lines report the log-linear fit; the slope reports the intensity change (in dB units) recorded for each 10× increment of concentration. (a) First CD44 sensor; (b) second CD44 sensor; (c) first HER2 sensor; (d) second HER2 sensor.
Supplier Page from Abcam for Recombinant human CD44 protein (Active)