Shrimp Alkaline Phosphatase (rSAP) M0371S from New England Biolabs

Product Specs
ItemShrimp Alkaline Phosphatase (rSAP)
CompanyNew England Biolabs
Price $64.00 Inquire
Catalog NumberM0371S
Size500 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Generation of Allogeneic CAR ‐T Circumvents Functional Deficits in Patient‐Derived Autologous Product for GlioblastomaInternational Journal of CancerSeptember 15, 2026Sabra K. Salim, Muhammad Vaseem Shaikh, Jeffrey Wei, William T. Maich, Alisha Anand, Oliver Y. Tang, Minomi K. Subapanditha, Zahra Alizada, Yujin Suk, Manoj Singh, et al.pAAV‐EGFP (Cell Biolabs VPK406) with NotI (NEB) in the presence of shrimp alkaline phosphatase (NEB M0371) and purified through gel purification. CART133‐HDR repair construct was digested with NotI and ligated

(2) Protocol to determine oligomerization states of proteins in living cells using photon counting histogram analysisSTAR ProtocAugust 5, 2026Zixiao Li, Tyler Camp, Yushan Li, Kai Zhangbuffer,New England Biolabs,Cat#B0202S recombinant shrimp alkaline phosphatase,New England Biolabs,Cat#M0371S T4 DNA ligase,New England Biolabs,Cat#M0202S Q5 Site-Directed Mutagenesis Kit,New England Biolabs,Cat#E0554S InFusion

(3) An optimized CRISPR/Cas9-based genome editing platform enhances bacteriocin production in Lactiplantibacillus plantarumJ Biol EngJuly 29, 2026Rajat Anand, Rudolf Lütticken, Laura De Laporte, Andreas Herrmann, Elisabeth HeineT4 Polynucleotide Kinase (T4 PNK NEB, Cat. no. M0201S), shrimp alkaline phosphatase (rSAP, NEB Cat. no. M0371S) were used for the phosphorylation and dephosphorylation reactions, respectively. Plasmid DNA was isolated

(4) ABL kinase inactivation induces transcription-replication conflicts and impairs replication fork progression in metastatic small cell lung cancerCell ReportsJuly 28, 2026Jing Jin Gu, Kevin M. Scott, Arijit Ghosh, Roberto H. Barbier, Douglas C. Rouse, Jacob P. Hoj, Michael W. Caminear, Fengqi Zhang, Boya Gao, Margaret W. Barbier, et al.followed by restriction digestion with NEB PaqCI (Cat# R0745) and dephosphorylation with NEB rSAP (Cat# M0371). Oligos (primer F: 5’- CCCAAgtC taCgaActActGgaAaa-3’, R: 5’-GTCCttTtcCagTagTtcGtaGacT-3’) were hybridized

(5) The Legionella pneumophila effector SidL is an adenylyltransferase that modifies the glycolytic intermediate 3-phosphoglycerateMol CellJuly 24, 2026Joshua J. Black, A. Maxwell Burroughs, Edrees H. Rashan, Charles R. Nosal, Katerina A. Romanov, Marco A. Catipovic, Catherine Valadez, L. Aravind, Kevin G. Hicks, Caren L. Freel Meyers, et al.Reactions were subsequently split in half and supplemented with either 0.5 μL rSAP (New England Biolabs #M0371) or rSAP storage buffer (25 mM Tris-HCl pH 7.5, 1 mM MgCl2, 50% glycerol) and incubated for an additional

(6) Blocking the m6Am methyltransferase PCIF1 releases STAT1-mediated Th1 immunity to potentiate cancer immunotherapyNat CommunJuly 22, 2026Jiansong Huang, Jing Zhou, Xiao Liu, Xiaoxu Liu, Ye Xiao, Kai Li, Chenbo Ding, Xuemin Cai, Wenli Mao, Changfen Xu, et al.ammonium acetate (pH 5.3) at 42 °C for 6 h. Next, 2.5 μl 0.5 M MES buffer (pH 6.5) and 1 U rSAP (NEB, M0371S) were added, and the mixture was further incubated at 37 °C for 6 h. For other RNA modifications, 150

(7) Validation of the mRNA epitranscriptome: SCARPET reveals that mapped m1A sites are inosineEMBO ReportsJune 23, 2026Rohit Nalavade, Aashiq H Mirza, Samie R Jaffrey[New England Biolabs, Cat # M0297L], and 1 U (1 μL) shrimp alkaline phosphatase (rSAP, New England Biolabs, Cat # M0371L) were added to each sample. The samples were incubated at 44 °C for 1 h. The RNase H and rSAP were then

(8) Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-CaptureBio ProtocJune 20, 2026Eleanor I. Lamont, Richard M. Jones, Jessica Assadi, Shuyi Ma, David R. Shermancatalog number: A63882) 9. Shrimp alkaline phosphatase (rSAP) and rCutSmart buffer (NEB, catalog number: M0371S) 10. Terminal transferase and cobalt (II) chloride (CoCl2) (NEB, catalog number: M0315S) 11. Biotin-11-ddATP

(9) CRISPR activation of endogenous PKD1 increases polycystin-1 levels and suppresses cellular features of ADPKDbioRxivMay 27, 2026Anubhav Chakraborty, Meekha M. Varghese, Darren P. Wallace, Christopher J. Ward, Alan S.L. Yulinearized with Esp3I enzyme (NEB, R0734S), dephosphorylated with shrimp alkaline phosphatase (NEB, M0371S), and gel-purified. Annealed oligos were ligated into the linearized vector and transformed into XL-1

(10) Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencingNucleic Acids ResMay 14, 2026Jingru Yu, Lauren S Ahmann, Yvette Y Yao, Angus Toland, Alicia Snowden, Chandler Ho, Netanel Loyfer, Tommy Kaplan, Hannes Vogel, Linlin Wang, et al.water. After adapter A purification, we removed residual phosphates using 1 µL of Shrimp Alkaline Phosphatase (M0371L, NEB) in 2 µL of 10x NEB buffer 2. The incubation was 1 h at 37°C, followed by 65°C for 10 min to quench

(11) Intracellular GRP78-Directed Delivery of Rapamycin by Biomolecular Condensates of Hydra-Elastin-like PolypeptidesBiomacromoleculesMay 11, 2026Sara Aly Attia, Sambid Adhikari, Amy S. Lee, John Andrew MacKayvector was incubated with NdeI and alkaline phosphatase (New England Biolabs, Ipswich, MA, USA #R0111, #M0371) for digestion and self-ligation inhibition, respectively. The L-peptide oligo with sticky ends was

(12) OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopyNucleic Acids ResApril 28, 2026Sara De Bragança, Clara Aicart-Ramos, Ángel Rivera-Calzada, Raquel Arribas-Bosacoma, Mark S Dillingham, Oscar Llorca, Fernando Moreno-Herrerodigested with SalI (New England Biolabs, #R0138S), dephosphorylated using rSAP (New England Biolabs, #M0371S), and without further purification, ligated with the fragment using T4 DNA ligase (New England Biolabs,

(13) Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cellsPLOS BiologyApril 27, 2026Jian Guan, Rebecca Lee Hurto, Akash Rai, Janakraj Bhattrai, Christopher A. Azaldegui, Luis A. Ortiz-Rodríguez, Quancheng Liu, Julie S. Biteen, Lydia Freddolino, Ursula Jakobtube. The 5′ phosphates were removed from the RNA by rSAP (1× rCutsmart buffer, 3 units of rSAP, (NEB, M0371)); 80 units of murine RNase Inhibitor (NEB, M0314) incubated for 2 h at 37 °C. RNA was cleaned by the

(14) Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone MethylationAdvanced ScienceApril 21, 2026Antoni Gañez‐Zapater, Savvas Kourtis, Camilla Reiter Elbæk, Lorena Espinar, Carolina Toro‐Márquez, Albert Coll‐Manzano, Alfredo Smiriglia, Laura García‐López, Laura Wiegand, Maria Guirola, et al.pLL3.7m‐Clover‐Geminin(1‐110)‐IRES‐mKO2‐Cdt were cut with MluI‐HF (NEB, R3198S), followed by dephosphorylation with rSAP (NEB, M0371S). The cut vectors were gel‐purified using the QIAquick Gel Extraction Kit (Qiagen, 28706). The PCR products

(15) Expanded applications of bioluminescence microscopy with phasor analysisCell Reports MethodsApril 20, 2026Lila P. Halbers, Caroline K. Brennan, Lorenzo Scipioni, Giulia Tedeschi, Zachary R. Torrey, Kshitij Parag-Sharma, Bryan Labra, Christoph Gohlke, Antonio L. Amelio, Michelle A. Digman, et al.resulting linearized vector was dephosphorylated using recombinant Shrimp Alkaline Phosphatase (NEB, cat #M0371). The digested/dephosphorylated plasmid was column purified (Macherey-Nagel PCR clean up kit, cat. #:

(16) Protocol for Using CRISPR-Cas9 to Generate a Monocyte Cell Line Harboring a Single-Nucleotide PolymorphismBio ProtocApril 20, 2026Amar Chandra Mahatha, Octavio Ramos-Espinosa, Dhemerson Souza de Lima, Ethan Y. Liu, Sarah Vaidyan, Jianguo Liu(Invitrogen, catalog number: 15224-017) 6. Alkaline phosphatase (rSAP) (New England BioLabs, catalog number: M0371S) 7. RPMI-1640 (Sigma-Aldrich, catalog number: R8758) 8. Fetal bovine serum (FBS) (Sigma-Aldrich, catalog

(17) Protocol for Using CRISPR-Cas9 to Generate a Monocyte Cell Line Harboring a Single-Nucleotide PolymorphismBIO-PROTOCOLApril 20, 2026Amar Chandra Mahatha, Octavio Ramos-Espinosa, Dhemerson Souza De Lima, Ethan Y. Liu, Sarah Vaidyan, Jianguo Liu(Invitrogen, catalog number: 15224-017) 6. Alkaline phosphatase (rSAP) (New England BioLabs, catalog number: M0371S) 7. RPMI-1640 (Sigma-Aldrich, catalog number: R8758) 8. Fetal bovine serum (FBS) (Sigma-Aldrich, catalog

(18) SFPQ Promotes Homologous Recombination via mRNA Stabilization of RAD51 and Its ParalogsJournal of Molecular BiologyApril 15, 2026Sofia Gotthold, Keile R. Hansen, Andrew N. Brown, Soham P. Chowdhury, Christine M. Joyce, Viraj Vinish, Sofie I. Rodriguez, Sambhav Jain, Hannah I. Ghasemi, Amanda C. Yoon, et al.triphosphate groups were removed by incubation with Shrimp Alkaline Phosphatase (New England Biolabs, Cat#M0371S), and gRNAs were purified using the RNeasy Mini Kit (QIAGEN, Cat#74106) for small-scale production or

(19) A survey of dietary effects on tRNA abundance and modificationsbioRxivApril 12, 2026Simeiyun Liu, Andrew D. Holmes, Ruoxia Zhao, Cassandra Herbert, Patrick A. Limbach, Todd M. Lowe, Upasna Sharmaminutes, followed by the addition of 5 mM MgCl2 and 0.5 units of shrimp alkaline phosphatase (NEB, cat# M0371S) at 37°C for 15 minutes, then stopped by the addition of 5 mM EGTA, and incubated at 65°C for 5 minutes.

(20) HP-Bodies – Ancestral Condensates that Regulate RNA Turnover and Protein Translation in BacteriabioRxivApril 11, 2026Jian Guan, Rebecca Lee Hurto, Akash Rai, Christopher A. Azaldegui, Luis A. Ortiz-Rodríguez, Julie S. Biteen, Lydia Freddolino, Ursula Jakobtube. The 5’ phosphates were removed from the RNA by rSAP (1X rCutsmart buffer, 3 units of rSAP, [NEB, M0371]; 80 units of murine RNase Inhibitor [NEB, M0314]) incubated for 2 hr at 37°C. RNA was cleaned by the

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Shrimp Alkaline Phosphatase (rSAP)

Shrimp Alkaline Phosphatase (rSAP) from New England Biolabs

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Shrimp Alkaline Phosphatase (rSAP) from New England Biolabs

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