E.coli RNA Polymerase, Core Enzyme M0550S from New England Biolabs

Product Specs
ItemE.coli RNA Polymerase, Core Enzyme
CompanyNew England Biolabs
Price $200.00 Inquire
Catalog NumberM0550S
Size100 units
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) An uncharacterized small protein MicN mediates the transcriptional reprogramming of Salmonella through regulating the RpoS-RNA polymerase interactionCommun BiolOctober 23, 2025Ruirui Liu, Qi Wang, Xilu Yuan, Yingying Yue, Ping Zhang, Kun Yin, Weiwei Wang, Bingqing Liafter incubation with EZ-Link-Biotin at 25 °C for 30 min, and then 0.1 µM RNAP (New England Biolabs, M0550S) was loaded onto each sensor in PBS. To study the effect of MicN on the interaction between RpoS and

(2) Fructose-responsive regulation by FruR in Faecalibacterium prausnitzii for its intestinal colonizationCommun BiolMarch 13, 2025HyeLim Choi, Sang-Hyun Cho, Joo-Hong Park, Yeong-Jae Seoktemplate, 2 µg FruR, and either 1 mM F1P or 5 µg HPr2 in a 36 µl volume with RNA polymerase buffer (NEB #M0550S). After a 15 min pre-incubation at 37 °C, NTPs (0.5 mM each, Invitrogen #18109017), 40 units of RNaseOUT™

(3) Citrate serves as a signal molecule to modulate carbon metabolism and iron homeostasis in Staphylococcus aureusPLOS PathogensJuly 30, 2024Feifei Chen, Qingmin Zhao, Ziqiong Yang, Rongrong Chen, Huiwen Pan, Yanhui Wang, Huan Liu, Qiao Cao, Jianhua Gan, Xia Liu, et al.polymerase holoenzyme (RNAP) was reconstituted by incubating the E. coli RNAPcore (1U/μl, NEB, Catalog no.M0550S) with same volume of S. aureus SigA (28 μM as stock) on ice for 15 min prior to use. For in vitro transcription,

(4) HPr prevents FruR-mediated facilitation of RNA polymerase binding to the fru promoter in Vibrio choleraeNucleic Acids ResearchJune 23, 2023Chang-Kyu Yoon, Seung-Hwan Lee, Jing Zhang, Hye-Young Lee, Min-Kyu Kim, Yeong-Jae Seokreaction mixture containing RNA polymerase reaction buffer (New England Biolabs, Beverley, MA, USA, #M0550S). The mixture was supplemented with a mixture of nucleotide triphosphate (NTP) (0.5 mM each NTP) (Invitrogen,

(5) A trailing ribosome speeds up RNA polymerase at the expense of transcript fidelity via force and allosteryCellMarch 16, 2023Liang Meng Wee, Alexander B. Tong, Alfredo Jose Florez Florez Ariza, Cristhian Cañari-Chumpitaz, Patricia Grob, Eva Nogales, Carlos J. Bustamanteanhydrous",Sigma-Aldrich,Cat#276855; CAS: 67-68-5 "E. coli RNA Polymerase, Core Enzyme",New England Biolabs,Cat#M0550S "1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDC",Thermo Fisher Scientific,Cat#22980;

(6) Engineered Cas12i2 is a versatile high-efficiency platform for therapeutic genome editingNature communicationsMay 20, 2022McGaw C, Garrity AJ, Munoz GZ, Haswell JR, Sengupta S, Keston-Smith E, Hunnewell P, Ornstein A, Bose M, Wessells Q, et al.E. coli RNA polymerase core enzyme (1 U/μL) and murine RNase inhibitor (40 U/μL) (New England Biolabs #M0550S and #M0314S). To set up each 1.2 μL reaction in a 384-well plate, a MANTIS Microfluidic Liquid Handler

(7) Redefining the Clostridioides difficile σ ^B Regulon: σ ^B Activates Genes Involved in Detoxifying Radicals That Can Result from the Exposure to Antimicrobials and Hydrogen PeroxidemSphereOctober 28, 2020Ilse M. Boekhoud, Annika-Marisa Michel, Jeroen Corver, Dieter Jahn, Wiep Klaas Smits(Macherey-Nagel). In vitro transcription reactions mixtures contained 1 μl (1 U) E. coli RNAPcore (catalog no. M0550S; NEB), 16 pmol sigma factor, 0.5 pmol DNA, 10 mM nucleoside triphosphate (NTP) mix, and 0.3 μl α-32P-ATP

(8) Ligand Modulates Cross-Coupling between Riboswitch Folding and Transcriptional PausingMolecular CellNovember 1, 2018Julia R. Widom, Yuri A. Nedialkov, Victoria Rai, Ryan L. Hayes, Charles L. Brooks, Irina Artsimovitch, Nils G. Walterligase 2,New England Biolabs,Cat#M0239S "E. coli RNA polymerase, core enzyme",New England Biolabs,Cat#M0550S "E. coli RNA polymerase, holoenzyme",New England Biolabs,Cat#M0551S "E. coli RNA polymerase, core enzyme

(9) Zinc-dependent regulation of zinc import and export genes by ZurNature CommunicationsAugust 1, 2017Seung-Hwan Choi, Kang-Lok Lee, Jung-Ho Shin, Yoo-Bok Cho, Sun-Shin Cha, Jung-Hye Roeprimers. Purified DNA (0.05 pmole), Zur (1 pmole), E. coli RNA polymerase core enzyme (1 Unit; NEB, M0550S), and sigma factor HrdB from S. coelicolor (1.12 μM) were incubated in 20 μl transcription buffer (20

(10) Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding proteinNucleic Acids ResearchJuly 27, 2017Aline Tabib-Salazar et al.involving recombinant proteins, the core and holoenzyme forms of the Ec RNAP were purchased from NEB; M0550S and M0551S, respectively and ∼0.02 mg was added to resin containing Gp5.7 and incubated for 1 h at 4°C.

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E.coli RNA Polymerase, Core Enzyme

E.coli RNA Polymerase, Core Enzyme from New England Biolabs

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E.coli RNA Polymerase, Core Enzyme from New England Biolabs

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