E. coliRNA Polymerase, Holoenzyme M0551S from New England Biolabs

Product Specs
ItemE. coliRNA Polymerase, Holoenzyme
CompanyNew England Biolabs
Price $150.00 Inquire
Catalog NumberM0551S
Size50 units
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dye-cycling DNA origami rotors for long-term tracking of transcription at base-pair resolutionbioRxivJanuary 28, 2026Amanda Wacker, Ryan J. Fantasia, Brian J. Tenner, Jerry Wu, Nicholas A. Monell, Pallav Kosuriexact concentration. RNAP stalling and ligation to rotors E. coli RNA polymerase holoenzymes (NEB M0551S) were stalled on a DNA template of interest using as previously described for studying synchronized

(2) High-throughput functional profiling and evolutionary covariation analysis of entire riboswitch sequencesbioRxivJanuary 16, 2026Laura M. Hertz, Anibal Arce, Elena Rivas, Julius B. Lucksallow open complex formation, 2.5 pmol of template DNA was incubated with 2 units of E. coli RNAP (NEB, M0551S) in 20 μL of buffer containing 62 mM KCl, 1.25 mM DTT, 25 mM Tris pH 8, 6.25 mM MgCl2, 0.125 mM EDTA,

(3) Structure of the 30S translation initiation complex coupled to paused RNA polymerase and its potential for riboregulationNature CommunicationsDecember 13, 2025Johann J. Roske, Giulia Paris, Akanksha Goyal, Marina Rodnina, Nikolay Zenkin, Katarzyna J. Bandyra, Ben F. Luisireactions containing 5 nM ompD-pMMB67HE, 0.5 U/μL RNaseOUT™ and 0.5 U E. coli RNAP Holoenzyme (NEB, M0551S) were pre-incubated in reaction buffer (40 mM TRIS, pH 7.5, 150 mM KCl, 10 mM MgCl2, 1 mM DTT, 0.01

(4) Structural basis of RNA-guided transcription by a dCas12f-σ E -RNAP complexbioRxivJune 13, 2025Renjian Xiao, Florian T. Hoffmann, Dan Xie, Tanner Wiegand, Adriana I. Palmieri, Samuel H. Sternberg, Leifu ChangDTT. Purified fractions were concentrated and stored at −80 °C. The E. coli RNAP–σ70 holoenzyme (NEB, M0551S) was purchased as a control for detecting chimeric Fta-Eco RNAP formation. Complex assembly. The dCas12f-gRNA-target

(5) Fructose-responsive regulation by FruR in Faecalibacterium prausnitzii for its intestinal colonizationCommun BiolMarch 13, 2025HyeLim Choi, Sang-Hyun Cho, Joo-Hong Park, Yeong-Jae Seok#18109017), 40 units of RNaseOUT™ (Invitrogen #10777019), and E. coli RNA polymerase holoenzyme (NEB #M0551S) were added, and the mixture was incubated for 1 h at 37 °C. DNA was then digested with DNase I (NEB

(6) Construction of multilayered gene circuits using de-novo-designed synthetic transcriptional regulators in cell-free systemsJournal of Biological EngineeringNovember 5, 2024Mingming Zhao, Jeongwon Kim, Jiayan Jiao, Yelin Lim, Xianai Shi, Shaobin Guo, Jongmin KimE. coli. All the gene fragments (Fuzhou Sunya Biotechnology) were ligated using Golden Gate assembly (#M0551S, New England Biolabs) and the ligated product was transformed into E. coli DH5α strain and cultured

(7) Characterization of the dual regulation by a c-di-GMP riboswitch Bc1 with a long expression platform from Bacillus thuringiensisMicrobiology SpectrumJuly 2, 2024Lu Liu, Dehua Luo, Yongji Zhang, Dingqi Liu, Kang Yin, Qing Tang, Shan-Ho Chou, Jin Hedeletion. The in vitro transcription reaction was as follows: First, 2 U of E. coli RNAP holoenzyme (M0551S, New England Biolabs), 2 uL of rNTP mix (N0466S, New England Biolabs, 25 mM of each), and 40 U of recombinant

(8) The effect of pseudoknot base pairing on cotranscriptional structural switching of the fluoride riboswitchNucleic Acids ResearchMay 8, 2024Laura M Hertz, Elise N White, Konstantin Kuznedelov, Luyi Cheng, Angela M Yu, Rivaan Kakkaramadam, Konstantin Severinov, Alan Chen, Julius B LucksMgCl2, 0.25 μl BSA (NEB, #B9000S), 100 nM DNA template and 2 μl E. coli RNA polymerase holoenzyme (NEB, #M0551S). Either 1 mM of NaCl or NaF was also included depending on the experimental conditions. Once assembled

(9) The Effect of Pseudoknot Base Pairing on Cotranscriptional Structural Switching of the Fluoride RiboswitchbioRxivDecember 5, 2023Laura M Hertz, Elise N White, Konstantin Kuznedelov, Luyi Cheng, Angela M Yu, Rivaan Kakkaramadam, Konstantin Severinov, Alan Chen, Julius B LucksKCl, 5 mM MgCl2, 0.25 μL BSA, 100 nM DNA template, and 2 μL E. coli RNA polymerase holoenzyme (NEB, #M0551S). Either 1 mM of NaCl or NaF was also included depending on the experimental conditions. Once assembled

(10) Isolation of E. coli RNA polymerase transcription elongation complexes by selective solid-phase photoreversible immobilizationMethods in EnzymologyNovember 1, 2023Eric J. Strobel3.1)E. coli RNA polymerase holoenzyme [purified in-house (See note 3) or New England Biolabs catalog no. M0551S], store at −20 °C100 mM MgCl2, store at RT2 mg/ml rifampicin in DMSO, store at −20 °C10% Triton X-100,

(11) High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for the Mycobacterium tuberculosis RNA polymeraseNucleic Acids ResearchOctober 27, 2023Drake Jensen, Ana Ruiz Manzano, Maxwell Rector, Eric J Tomko, M Thomas Record, Eric A GalburtE. coli RNAP σ70 holoenzyme was purchased from New England BioLabs, Inc. (Ipswich, MA; catalogue # M0551S). Purified E. coli GreB was a generous gift from Irina Artsimovitch (The Ohio State University) (47).

(12) Structural basis for control of bacterial RNA polymerase pausing by a riboswitch and its ligandNature Structural & Molecular BiologyJuly 1, 2023Adrien Chauvier, Jason C. Porta, Indrajit Deb, Emily Ellinger, Katarina Meze, Aaron T. Frank, Melanie D. Ohi, Nils G. Walterprimer (Trilink, O-31004), and 50 nM DNA template. 100 nM E. coli RNAP holoenzyme (New England Biolabs, M0551S) was added to the reaction mixture and incubated for 10 min at 37°C. The reaction mixture was then passed

(13) High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for Mycobacterium tuberculosis RNA polymerasebioRxiv : the preprint server for biologyMarch 13, 2023Jensen D, Manzano AR, Rector M, Tomko EJ, Record MT, Galburt EA.holoenzyme was purchased from New England BioLabs, Inc. at 1.7 μM concentration (Ipswich, MA; catalog # M0551S). Purified E. coli GreB was a generous gift from Irina Artsimovitch (The Ohio State University) (87).

(14) Redesigning regulatory components of quorum-sensing system for diverse metabolic controlNature CommunicationsDecember 1, 2022Chang Ge, Zheng Yu, Huakang Sheng, Xiaolin Shen, Xinxiao Sun, Yifei Zhang, Yajun Yan, Jia Wang, Qipeng Yuanpurified in E. coli55. The used RNAP was NEB® product, holoenzyme of E. coli RNA polymerase (Catalog #: M0551S). Electrophoretic mobility shift assay The Electrophoretic mobility shift assay (EMSA) experiments

(15) Promiscuity of response regulators for thioredoxin steers bacterial virulenceNature communicationsOctober 20, 2022Kim JS, Born A, Till JKA, Liu L, Kant S, Henen MA, Vögeli B, Vázquez-Torres A.Thermo Fisher Scientific), 1 nM ompF DNA templates (Table S3), 5 nM E. coli holoenzyme RNA polymerase (cat. # M0551S, New England Biolabs, Ipswich, MA) and 2.5 µM of OmpR variants61. Reactions were incubated at 37 °C

(16) Rho-dependent transcription termination proceeds via three routesNature CommunicationsMarch 29, 2022Eunho Song, Heesoo Uhm, Palinda Ruvan Munasingha, Seungha Hwang, Yeon-Soo Seo, Jin Young Kang, Changwon Kang, Sungchul Hohngbuffer. Preparation of E. coli proteins RNAP holoenzyme was either purchased from New England Biolab (M0551S) or custom-purified as previously described41,42. ρ factor was purchased from Bioprogen. Three ρ mutants

(17) Genome-wide binding potential and regulatory activity of the glucocorticoid receptor's monomeric and dimeric formsNature communicationsMarch 31, 2021Johnson TA, Paakinaho V, Kim S, Hager GL, Presman DM.barcoded Ad2.x (see list below) primers, and NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs #M0551S). After 5x PCR amplification cycles, appropriate number of additional PCR cycles was determined to retain

(18) SUMOylation regulates the protein network and chromatin accessibility at glucocorticoid receptor-binding sitesNucleic acids researchFebruary 26, 2021Paakinaho V, Lempiäinen JK, Sigismondo G, Niskanen EA, Malinen M, Jääskeläinen T, Varjosalo M, Krijgsveld J, Palvimo JJ.barcoded using published primers (42), and NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs, M0551S). Appropriate number of PCR cycles was determined as described (42). After PCR, the samples were size

(19) Computationally Reconstructing Cotranscriptional RNA Folding Pathways from Experimental Data Reveals Rearrangement of Non-Native Folding IntermediatesMolecular CellFebruary 1, 2021Angela M Yu, Paul M. Gasper, Luyi Cheng, Lien B. Lai, Simi Kaur, Venkat Gopalan, Alan A. Chen, Julius B. LucksDeoxynucleotide (dNTP) Solution Mix,New England Biolabs,Cat#N9447L "E. coli RNA Polymerase, Holoenzyme",New England Biolabs,Cat#M0551S Gln111 (EcoRI E111Q Mutant),Lab preparation,N/A Ribonucleotide Solution Set,New England Biolabs,Cat#N0466 Benzoyl

(20) Kinetic Modeling and Meta-Analysis of the Bacillus subtilis SigB Regulon during Spore Germination and OutgrowthMicroorganismsJanuary 5, 2021Jiri Vohradsky, Marek Schwarz, Olga Ramaniuk, Olatz Ruiz-Larrabeiti, Viola Vaňková Hausnerová, Hana Šanderová, Libor Krásný0.1 M NaCl, 50% glycerol) for 10 min at 37 °C. E. coli RNAP holoenzyme was purchased from NEB (cat# M0551S). Multiple round transcription reactions were carried out in 10 μL reaction volumes with 60 nM B. subtilis

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E. coliRNA Polymerase, Holoenzyme

E. coliRNA Polymerase, Holoenzyme from New England Biolabs

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E. coliRNA Polymerase, Holoenzyme from New England Biolabs

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