Blunt/TA Ligase Master Mix M0367L from New England Biolabs

Product Specs
ItemBlunt/TA Ligase Master Mix
CompanyNew England Biolabs
Price $427.00 Inquire
Catalog NumberM0367L
Size250 reactions
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Phage-assisted evolution of allosteric protein switchesNat CommunApril 14, 2026Nicholas T. Southern, Anna von Bachmann, Alisa Hovsepyan, Marielouise Griebl, Benedict Wolf, Nina Lemmen, Ann-Sophie Kroell, Simon Westermann, Jan Mathony, Dominik Niopekby barcode adapter ligation with the NEB Blunt/TA Ligase Master Mix (New England Biolabs, cat. no. M0367L). Barcoded samples were pooled, purified with AMPure XP beads, and ligated to sequencing adapters using

(2) Pathogenic CGG expansions in oculopharyngodistal myopathy exhibit distinct characteristics of each causative gene on the flanking sequences as well as methylation statusGenome MedMarch 27, 2026Nobuyuki Eura, Satoru Noguchi, Megumu Ogawa, Kyuto Sonehara, Ai Yamanaka, Takashi Kurashige, Shinichiro Hayashi, Yukinori Okada, Kazuma Sugie, Ichizo Nishinoligate barcodes to the cleaved and dA-tailed genomic DNA samples with Blunt/TA Ligase Master Mix (NEB, #M0367L) at room temperature for 10 min, followed by purification on a magnet using Agencourt AMPure XP Beads

(3) MASTR-seq enables multiplexed analysis of short tandem repeats with sequencingCell Rep MethodsMarch 23, 2026Chuanbin Su, Han-Seul Ryu, Keerthivasan Raanin Chandradoss, Thomas Malachowski, Ravi Boya, Linda Zhou, Hoa Emma Nguyen, Esteban O. Mazzoni, Kristen J. Brennand, Jennifer E. Phillips-CreminsQuick-Load 1 kb Extend DNA Ladder,New England Biolabs,Cat# N3239S NEB Blunt/TA Ligase Master Mix,New England Biolabs,Cat# M0367L "T4 DNA ligase, from NEBNext Quick Ligation Module",New England Biolabs,Cat# E6056S EcoRI,New England Biolabs

(4) Transient telomere uncapping triggers telomeric and subtelomeric rearrangementsEMBO ReportsFebruary 17, 2026Liébaut Dudragne, Clotilde Garrido, Oana Ilioaia, Juliana Silva Bernardes, Zhou XuOxford Nanopore Technologies,New England Biolabs,E7180S Blunt/TA ligase master mix,New England Biolabs,M0367L SeaKem GTG Agarose,Lonza,50070 Phenol:chloroform:isoamyl (25:24:1),Sigma-Aldrich,P3803 XhoI FastDigest,Thermo Fisher Scientific

(5) Sensitive, flexible, and affordable serum RNA sequencing for pathogen detection on the Oxford Nanopore platformBMC GenomicsFebruary 12, 2026Philippe Selhorst, Emilie Van Vyve, Francesca Falconi-Agapito, Joachim Mariën, Kevin K. Ariëneluted in 25 µL of water. Adapter ligation was performed by adding 25 µL blunt/TA ligase master mix (M0367L, NEB) and 5 µL of ligation adapter (SQK-LSK114, ONT) to 62 ng of eluate followed by 15 min incubation

(6) Unraveling the intra-species genomic diversity of sweetpotato-infecting CRESS-DNA and RNA viruses in Burkina Faso using Oxford Nanopore sequencingFrontiers in MicrobiologyFebruary 4, 2026Pakyendou E. Name, Ezechiel B. Tibiri, Fidèle Tiendrébéogo, Seydou Sawadogo, Florencia Djigma, Lassina Traoré, Angela O. Eni, Justin S. Pitaidentification of reads from each sample. Blunt/TA Ligase Master Mix (New England Biolabs, MA, USA, Cat. No. M0367L) was used to ligate the barcodes to the DNA fragments. After pooling all samples, adapter ligation was

(7) Hybrid genome sequence of Cryptococcus neoformans of Indian origin and comparative genome analysisScientific ReportsDecember 4, 2025Jananishree Sathiyamoorthy, Jayapradha RamakrishnanA-tailed (NEBnext ultra II end repair kit, New England Biolabs, MA, USA). A blunt TA ligase master mix (M0367L) barcodes the end-prepared samples. NEB Quick T4 DNA Ligase ligated the sequencing adapters (SQK-NBD114)

(8) Genomic insights into diversity, antimicrobial resistance and virulence of Glaesserella parasuis from diseased swine in PeruFrontiers in MicrobiologySeptember 23, 2025Luis Alvarez-Vega, Karolina S. M. De La Cruz-Monroy, Arturo Garcia-Barraza, Dennis Carhuaricra, Jose L. Llaja-Bernedo, Liseth L. Huamancha-Pulido, Alejandra Medina-Gonzales, Andre F. Sedano-Sanchezbarcodes (SQK-NBD114.24, Oxford Nanopore, UK) were ligated to DNA fragments using the Blunt/TA Master Mix (M0367L, New England Biolabs, USA). The samples were then cleaned with magnetic beads and 80% ethanol to remove

(9) Investigating the molecular transmission dynamics of bla NDM in antibiotic-selective environmentsJournal of BacteriologySeptember 18, 2025Shashi Kumari, Lekshmi Narendrakumar, Meenal Chawla, Sanjib Das, Hemanta Koley, Bhabatosh DasNew England Biolabs, MA, USA). The end-prepared samples were barcoded using Blunt TA ligase master mix (M0367L). The equimolar concentration of the barcoded sample was pooled, and the library was prepared by ligating

(10) Effects of Aging on Z-DNA-Induced Genetic Instability In VivoGenes (Basel)August 11, 2025Tonia T. Li, Alexandra D’Amico, Laura Christensen, Karen M. Vasquezmutation reporter was then treated with 2× Blunt/TA ligase master mix (NEB, Ipswich, MA, USA, REF: M0367L) for one hour at 25 °C, purified via phenol–chloroform extraction and ethanol precipitation, and resuspended

(11) Chromatin architecture mapping by multiplex proximity taggingMolecular CellJuly 17, 2025Axel Delamarre, Benton Bailey, Jennifer Yavid, Richard Koche, Neeman Mohibullah, Iestyn Whitehouse10mM. 20μL of beads were incubated with 2μL of linker mixture at 25μM and 22μL Blunt/TA ligase (NEB, M0367L) for 40min at 20°C. Beads were washed twice in 500 μl of HB and twice in 500 μl of SB. Beads were resuspended

(12) Phylogenetic analysis of the Critically Endangered Aquilaria khasiana (Thymelaeaceae) using barcode markers and chloroplast genome, with updated conservation statusSci RepJuly 1, 2025Ranjith Layola M.R., Sanchayita Sengupta, Bidisha Mallick, Tanay Shil, Ranjan Shaw, Farheen Banu, Sayan Chakraborty, Sudipta Sardar, Oindrila Chakraborty, Sayak Chakraborty, et al.New England Biolabs, MA, USA). The end-prepared samples were barcoded using Blunt TA ligase master mix (M0367L). The equimolar concentration of the barcoded sample was pooled and sequencing adapters (SQK-NBD114.96)

(13) Optimizing fungal DNA extraction and purification for Oxford Nanopore untargeted shotgun metagenomic sequencing from simulated hemoculture specimensmSystemsJune 17, 2025Nattapong Langsiri, Wieland Meyer, Laszlo Irinyi, Navaporn Worasilchai, Nuttapon Pombubpa, Thidathip Wongsurawat, Piroon Jenjaroenpun, J. Jennifer Luangsa-ard, Ariya ChindampornBarcoding Expansion 1–12 (cat. no. EXP-NBD104, ONT, Didcot, UK) using Blunt/TA Ligase Master Mix (cat. no. M0367L, New England Biolabs, Ipswich, UK). Barcoded DNA samples were pooled equally, each at a concentration

(14) A transient mutational burst occurs during yeast colony developmentMolecular Systems BiologyJune 9, 2025Nicolas Agier, Nina Vittorelli, Louis Ollivier, Frédéric Chaux, Alexandre Gillet-Markowska, Samuel O’Donnell, Fanny Pouyet, Gilles Fischer, Stéphane DelmasTechnologies,FLO-MIN106D NEBnext companion module for Oxford Nanopore Technologies,NEB,E7180S Blunt/TA ligase master mix,NEB,M0367L Zymolyase 20 T,Euromedex,UZ1000 RNAse A,Euromedex,9707 Software,Software,Software R 3.6.3 / 4.1.0

(15) Draft genomes of Diaporthe ueckeri and Diaporthe longicolla strains associated with the soybean pod and grain rot disease in BrazilMicrobiology Resource AnnouncementsMay 8, 2025Hiago Antonio Oliveira da da Silva, Cosma Raissa B. Nascimento, Rafael S. Anjos, Eduardo S. G. Mizubutisize selection was performed. The end-prepared samples were barcoded using Blunt/TA ligase master mix (M0367L). The library was prepared by ligating sequencing adapters (SQKNBD114.24) onto double-stranded DNA fragments

(16) Optimizing nanopore adaptive sampling for pneumococcal serotype surveillance in complex samples using the graph-based GNASTy algorithmGenome ResearchApril 14, 2025Samuel T. Horsfield, Basil C.T. Fok, Yuhan Fu, Paul Turner, John A. Lees, Nicholas J. Croucherligation followed, using the barcodes provided and the NEB Blunt/TA Ligase Master Mix (New England Biolabs M0367L), with samples then being pooled together and cleaned as before. Finally, adapter ligation was conducted

(17) Tissue origin of endothelial cells determines immune system modulation and regulation of HIF-1α-, TGF-β-, and VEGF signalingiScienceFebruary 21, 2025Robin Heiden, Laura Hannig, Jakob S. Bernhard, Mario Vallon, Anja Schlecht, Nico Hofmann, Süleyman Ergün, Franziska Hoschek, Maximilian Wagner, Andreas Neueder, et al.assigned barcode by incubation of the sample together with the barcode and Blunt/TA Ligase Master Mix (#M0367L, NEB) for 10 min at 21°C. After another cleanup step, samples were eluted in 21.7 μL nuclease-free water

(18) Identification of Mycobacterium tuberculosis and its Drug Resistance by Targeted Nanopore Sequencing TechnologyBIO-PROTOCOLFebruary 5, 2025Chen Tang, Feng Xu, Xiaoqun Zheng, Guangxin Xiangcatalog number: SQK-NBD114.24) 15. NEB Blunt/TA ligase master mix (New England Biolabs, catalog number: M0367L) 16. NEB Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, catalog number: M0493L) 17.

(19) Commensal-pathogen dynamics structure disease outcomes during Clostridioides difficile colonizationCell Host & MicrobeJanuary 8, 2025Skye R. S. Fishbein, Anna L. DeVeaux, Sakshi Khanna, Aura L. Ferreiro, James Liao, Wesley Agee, Jie Ning, Bejan Mahmud, Miranda J. Wallace, Tiffany Hink, et al.#FLO-PRO114M Native Barcoding Kit 96 V14,ONT,Cat #SQK-NBD114.96 Blunt/TA Ligase Master Mix,NEB,Cat #M0367L NEBNext Quick Ligation Reaction Buffer,NEB,Cat #B6058S Companion Module for Oxford Nanopore Technologies®

(20) Chromosome level assemblies of Nakaseomyces (Candida) bracarensis uncover two distinct clades and define its adhesin repertoireBMC GenomicsNovember 7, 2024Marina Marcet-Houben, Ewa Księżopolska, Toni GabaldónAdapter Mix (AMX1D, ONT) was ligated to the purified samples using the Blunt/TA Ligase Master Mix (NEB, M0367L). When using the SQK-LSK308 kit, two adapter ligation steps were required. First, the 1D2 adapter was

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Blunt/TA Ligase Master Mix

Blunt/TA Ligase Master Mix from New England Biolabs

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Blunt/TA Ligase Master Mix from New England Biolabs

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