Blunt/TA Ligase Master Mix M0367S from New England Biolabs

Product Specs
ItemBlunt/TA Ligase Master Mix
CompanyNew England Biolabs
Price $107.00 Inquire
Catalog NumberM0367S
Size50 reactions
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C
Product TypeNGS Kits
Workflow StepAdapter Ligation

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Efficient whole-genome sequencing of Monkeypox virus using a novel nuclease-multiple displacement amplification enrichment methodnpj VirusesJanuary 21, 2026Masayasu Misu, Takeshi Kurosu, Tomoki Yoshikawa, Madoka Kawahara, Kohei Oishi, Masayuki Shimojima, Hideki Ebiharaa 20-μL reaction mixture including 8.5 μL of DNA, 10 μL of Blunt/TA Ligase Master Mix (catalog No. M0367S; NEB), and 1.5 μL of Native Barcoding Expansion. The reaction was incubated at room temperature (20–25

(2) Machine Learning‐Driven Nanopore Sensing for Quantitative, Label‐Free miRNA DetectionSmall MethodsJanuary 19, 2026Caroline Koch, Seshagiri Sakthimani, Victoria Maria Noakes, Miruna Cretu, David Newman, Richard Gutierrez, Mark Bruce, Julia Gorelik, Nadia Guerra, Joshua B. Edel, et al.7.5 µL ligation adapter from the ligation sequencing kit SQK‐LSK114 (ONT, UK) and 8.325 µL TA‐ligase (M0367S, New England Biolabs, USA), centrifuged for 1 min at 3000 rpm, and incubated for 20 min at RT on a HulaMixer. Purification

(3) Expression of ultralong complementarity determining region 3 and development of IgM and IgG B cell receptor repertoires in Holstein heifer calvesImmunoHorizonsJanuary 14, 2026Tess E Altvater-Hughes, Harold P Hodgins, Douglas C Hodgins, Cathy A Bauman, Bonnie A MallardUK, catalog no. SQK-NBD114.24) and the Blunt/TA Ligase Master Mix (New England Biolabs, catalog no. M0367S). Samples were washed and eluted in nuclease-free water. Adapters were added using the NEBNext Quick Ligation Module

(4) Enhanced cleavage of genomic CCR5 using CASX2MaxRNA BiolOctober 25, 2025Christine A. Hodge, Niles P. Donegan, David A. Armstrong, Mathew S. Hayden, Alexandra L. Howellend-repaired and cleaned up PCR product was barcoded with the provided barcodes using Blunt/TA ligase (M0367S, NEB). The barcoded PCR products were then pooled, cleaned up a second time with AMPure beads, and then

(5) Aeromonas spp. as a fast-growing high-performance chassis for protein productionApplied and Environmental MicrobiologyJuly 23, 2025Ming-Xuan Tang, Peng-Fei Meng, Ruo-Lin Huang, Xin Zheng, Chen-Chen Liang, Xuepiao Pu, Chen Wang, Ying Zhao, Yi-Qiu Zhang, Jia-Xin Liang, et al.end-repaired DNA was ligated with AdaptomrMix using the Blunt/TA Ligation Master Mix (New England Biolabs, #M0367S). The ligated DNA was further purified with AMPure XP beads and the ABB buffer supplied in the kit.

(6) Pu.1/Spi1 dosage controls the turnover and maintenance of microglia in zebrafish and mammalsElifeJuly 17, 2025Yi Wu, Weilin Guo, Haoyue Kuang, Xiaohai Wu, Thi Huong Trinh, Yuexin Wang, Shizheng Zhao, Zilong Wen, Tao Yurespectively. PCR products were ligated to pUCm-T vector (B522211, Sangon) with Blunt/TA Ligase Master Mix (M0367S, NEB) according to the manufacturer’s instructions. The clones containing ligated plasmids were amplified

(7) Uncalled4 improves nanopore DNA and RNA modification detection via fast and accurate signal alignmentNature MethodsMarch 28, 2025Sam Kovaka, Paul W. Hook, Katharine M. Jenike, Vikram Shivakumar, Luke B. Morina, Roham Razaghi, Winston Timp, Michael C. Schatzend-prepped fragments were mixed with PCR adapters (ONT, EXP-PCA001) and Blunt/TA Ligase Master Mix (NEB, M0367S) and incubated for 15 min at room temperature. DNA was then purified using 0.4× AMPure XP beads and

(8) Mutation of the LRG1 Rho-GAP gene is responsible for the hyper branching C-variant phenotype in the quorn mycoprotein fungus Fusarium venenatum A3/5Fungal Biol BiotechnolMarch 24, 2025John Connell, Helen J. Bates, Ivey Geoghegan, Fiona Wilson, Richard J. Harrison, R. Jordan Priceusing NEBNext End Repair (NEB, E6050S), NEBNext dA-tailing (NEB, E6053S) and Blunt T/A ligase (NEB, M0367S) modules. Libraries were multiplexed and sequenced using Illumina Miseq v3 2 × 300 bp PE (Illumina,

(9) Plasmid Library Construction From Genomic DNACurr ProtocJanuary 22, 2025Valeria Florez-Cardona, Jessica Khani, Emily McNutt, Bruno Manta, Mehmet BerkmenethanolAMPure magnetic beads (Beckman Coulter, cat. no. A63880)Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S)Linearized, purified vector (Support Protocol 2)10‐β competent E. coli (high efficiency; NEB, cat. no.

(10) Mapping protein–DNA interactions with DiMeLo-seqNature ProtocolsDecember 1, 2024Annie Maslan, Nicolas Altemose, Jeremy Marcus, Reet Mishra, Lucy D. Brennan, Kousik Sundararajan, Gary Karpen, Aaron F. Straight, Aaron StreetsAgencourt AMPure XP beads (Beckman Coulter, cat. no. A63881) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) NEBNext quick ligation module (NEB, cat. no. E6056S) NEBNext End Repair dA-tailing Module (NEB,

(11) Native RNA nanopore sequencing reveals antibiotic-induced loss of rRNA modifications in the A- and P-sitesNature CommunicationsNovember 29, 2024Anna Delgado-Tejedor, Rebeca Medina, Oguzhan Begik, Luca Cozzuto, Judith López, Sandra Blanco, Julia Ponomarenko, Eva Maria Novoato 25 °C (−0.1 °C/s). Later, 2.5uL from a single native barcode and 25 µL Blunt/TA Ligase Mix (NEB, M0367S) were added, followed by a 10 min incubation at RT and a clean up step with 0.5X AMPure XP beads and

(12) Investigating the IgM and IgG B Cell Receptor Repertoires and Expression of Ultralong Complementarity Determining Region 3 in Colostrum and Blood from Holstein-Friesian Cows at CalvingAnimals (Basel)October 2, 2024Tess E. Altvater-Hughes, Harold P. Hodgins, Douglas C. Hodgins, Cathy A. Bauman, Marlene A. Paibomesai, Bonnie A. MallardOxford, UK, catalog # SQK-NBD114.24) and the Blunt/TA Ligase Master Mix (New England Biolabs, catalog # M0367S). Samples were washed and eluted in nuclease-free water. Adapters were added using the NEBNext Quick

(13) Estimates of Sequences with Ultralong and Short CDR3s in the Bovine IgM B Cell Receptor Repertoire Using the Long-read Oxford Nanopore MinION PlatformImmunoHorizonsSeptember 9, 2024Tess E. Altvater-Hughes, Harold P. Hodgins, Douglas C. Hodgins, Natasha B. Gallo, Gabhan I. Chalmers, Nicole D. Ricker, Bonnie A. MallardUK, catalog no. SQK-NBD114.24) and the Blunt/TA ligase master mix (New England Biolabs, catalog no. M0367S). Samples were washed and eluted in nuclease-free water. Barcoded samples were then pooled, and adapters

(14) Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screensNature BiotechnologyAugust 1, 2024Zihan Xu, Andras Sziraki, Jasper Lee, Wei Zhou, Junyue Caolinearized backbone and the annealed duplex were ligated using the Blunt/TA Ligase Master Mix (NEB, M0367S). Transformation, clone amplification, sequencing validation, lentivirus generation and titer measurement

(15) Identification of PTPN12 Phosphatase as a Novel Negative Regulator of Hippo Pathway Effectors YAP/TAZ in Breast CancerInternational Journal of Molecular SciencesApril 5, 2024Sahar Sarmasti Emami, Anni Ge, Derek Zhang, Yawei Hao, Min Ling, Rachel Rubino, Christopher J. B. Nicol, Wenqi Wang, Xiaolong Yangannealed oligos andEsp3I/BsmBI-digested LentiGuide-Puro vector with Blunt/TA ligase master mix (NEB, # M0367S), followed by a heat shock transformation into a Stbl3 competent cell. 4.2. Cell Culture HEK293T (human

(16) Activity of zebrafish THAP9 transposase and zebrafish P element-like transposonsbioRxivMarch 27, 2024Nitzan Kutnowski, George E. Ghanim, Yeon Lee, Donald C. Rio,New England Biolabs,R0146S XmaI,New England Biolabs,R0180S Blunt/TA Ligase Master Mix,New England Biolabs,M0367S ,, Software,, Tcoffee-Expresso,"Di Tommaso et al., 2011", ITASSER,"Lee et al., 2007", Alphafold2,"Jumper

(17) Challenges in the discovery of tumor-specific alternative splicing-derived cell-surface antigens in gliomaSci RepMarch 16, 2024Takahide Nejo, Lin Wang, Kevin K. Leung, Albert Wang, Senthilnath Lakshmanachetty, Marco Gallus, Darwin W. Kwok, Chibo Hong, Lee H. Chen, Diego A. Carrera, et al.Agencourt AMPure XP beads (Beckman Coulter, A63880); Blunt/TA Ligase Master Mix (New England Biolabs, M0367S); NEBNext Quick Ligation Reaction Buffer (New England Biolabs, B6058S); NEBNext Companion Module for

(18) Thorough molecular configuration analysis of non-canonical AAV genomes in AAV vector preparationsMolecular Therapy - Methods & Clinical DevelopmentMarch 14, 2024Junping Zhang, Xiangping Yu, Matthew Chrzanowski, Jiahe Tian, Derek Pouchnik, Ping Guo, Roland W. Herzog, Weidong Xiao(New England Biolabs, catalog no. M0212S)Blunt/TA Ligase Master Mix (New England Biolabs, catalog no. M0367S)Overhang T-tailing adapter (synthesized by Integrated DNA Technology)Exonuclease III (New England Biolabs,

(19) Uncalled4 improves nanopore DNA and RNA modification detection via fast and accurate signal alignmentbioRxivMarch 10, 2024Sam Kovaka, Paul W. Hook, Katharine M. Jenike, Vikram Shivakumar, Luke B. Morina, Roham Razaghi, Winston Timp, Michael C. Schatzend-prepped fragments were mixed with PCR adapters (ONT, EXP-PCA001) and Blunt/TA Ligase Master Mix (NEB, M0367S) and incubated for 15 minutes at room temperature. DNA was then purified using 0.4x AMPure XP beads

(20) Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemicPLoS OneAugust 25, 2023Marine Wasniewski, Franck Boué, Céline Richomme, Etienne Simon-Lorière, Sylvie Van der Werf, Flora Donati, Vincent Enouf, Yannick Blanchard, Véronique Beven, Estelle Leperchois, et al.ng DNA from each sample. Native barcode adapters NBD04 were ligated in Blunt/TA Ligase Master Mix (M0367S, NEB, USA), and the resulting product was purified using AMPure XP beads before being pooled to produce

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Blunt/TA Ligase Master Mix

Blunt/TA Ligase Master Mix from New England Biolabs

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Blunt/TA Ligase Master Mix from New England Biolabs

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