Phusion Hot Start Flex DNA Polymerase M0535L from New England Biolabs

Product Specs
ItemPhusion Hot Start Flex DNA Polymerase
CompanyNew England Biolabs
Price $609.00 Inquire
Catalog NumberM0535L
Size500 units
ApplicationsDNA Analysis
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) MIC-Drop-seq: scalable single-cell phenotyping of mutant vertebrate embryosNature CommunicationsApril 1, 2026Clayton M. Carey, Saba Parvez, Zachary J. Brandt, Brent W. Bisgrove, Christopher J. Yates, Randall T. Peterson, James A. Gagnonbuffer, 200 µM dNTPs (Invitrogen, cat. no. 10297018), 3% DMSO, 1 U of Phusion polymerase (NEB, cat. no. M0535L). The fill-in reaction was performed in a thermal cycler at the following conditions: 98 °C for 2 min,

(2) An image-based transcriptomics atlas reveals the regional and microbiota-dependent molecular, cellular, and spatial structure of the murine gutCell Host MicrobeFebruary 19, 2026Rosalind J. Xu, Hao Zhang, Paolo Cadinu, Phillip B. Nicol, Uli S. Herrmann, Tyrone Lee, Ludwig Geistlinger, Rafael A. Irizarry, Jeffrey R. Moffittpreviously described protocol39. Briefly, the oligo pool was amplified using limited cycle PCR (NEB M0535L) and purified via spin column (Zymo Spin V with DNA Clean & Concentrator-5, C1012–50 and D4004). The

(3) An image-based transcriptomics atlas reveals the regional and microbiota-dependent molecular, cellular, and spatial structure of the murine gutbioRxivJuly 30, 2025Rosalind J. Xu, Paolo Cadinu, Phillip B. Nicol, Uli S. Herrmann, Tyrone Lee, Ludwig Geistlinger, Rafael A. Irizarry, Jeffrey R. Moffittpreviously described protocol37. Briefly, the oligo pool was amplified using limited cycle PCR (NEB M0535L) and purified via spin column (Zymo Spin V with DNA Clean & Concentrator-5, C1012–50 and D4004). The

(4) MIC-Drop-seq: Scalable single-cell phenotyping of mutant vertebrate embryosbioRxivMay 30, 2025Clayton M. Carey, Saba Parvez, Zachary J. Brandt, Brent W. Bisgrove, Christopher J. Yates, Randall T. Peterson, James A. Gagnonbuffer, 200 μM dNTPs (Invitrogen, cat. no. 10297018), 3% DMSO, 1 U of Phusion polymerase (NEB, cat. no. M0535L). The fill-in reaction was performed in a thermal cycler at the following conditions: 98 °C for 2 min,

(5) GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesisElifeMarch 13, 2025Joseph A Bisson, Miriam Gordillo, Ritu Kumar, Neranjan de Silva, Ellen Yang, Kelly M Banks, Zhong-Dong Shi, Kihyun Lee, Dapeng Yang, Wendy K Chung, et al.gRNA pairs following PCR amplification (Phusion Hot Start Flex DNA Polymerase, New England Biolabs M0535L) and agarose gel electrophoresis from genomic DNA isolated 2–3 days after electroporation using the

(6) GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesisElifeMarch 13, 2025Joseph A Bisson, Miriam Gordillo, Ritu Kumar, Neranjan de Silva, Ellen Yang, Kelly M Banks, Zhong-Dong Shi, Kihyun Lee, Dapeng Yang, Wendy K Chung, et al.gRNA pairs following PCR amplification (Phusion Hot Start Flex DNA Polymerase, New England Biolabs M0535L) and agarose gel electrophoresis from genomic DNA isolated 2–3 days after electroporation using the

(7) miRNA-target complementarity in cnidarians resembles its counterpart in plantsEMBO ReportsJanuary 2, 2025Yael Admoni, Arie Fridrich, Paris K Weavers, Reuven Aharoni, Talya Razin, Miguel Salinas-Saavedra, Michal Rabani, Uri Frank, Yehu MoranAlexa Fluor 4.88,Thermo Fisher Scientific,D22910 Red sea salt,Red sea, phenol red,New England Biolabs,M0535L Pronase,Merck Millipore,P5147 Tri-Reagent®,Merck Millipore,T9424 Turbo DNase,Thermo Fisher Scientific,AM1907 RNase-free

(8) Charting the cellular biogeography in colitis reveals fibroblast trajectories and coordinated spatial remodelingCellApril 2, 2024Paolo Cadinu, Kisha N. Sivanathan, Aditya Misra, Rosalind J. Xu, Davide Mangani, Evan Yang, Joseph M. Rone, Katherine Tooley, Yoon-Chul Kye, Lloyd Bod, et al.protocol49,51,59. Briefly, the oligopool was amplified using limited cycle PCR (New England Biolabs [NEB], M0535L) to produce a pool of template molecules for in vitro transcription. The resulting product was purified

(9) Large-scale F0 CRISPR screens in vivo using MIC-DropNature ProtocolsJune 1, 2023Saba Parvez, Zachary J. Brandt, Randall T. Peterson(Supplementary Table 1)(IDT)Phusion HS Flex DNA polymerase and 5x HF buffer (New England Biolabs; cat. no. M0535L)100 mM dNTP Set (Invitrogen, cat. no. 10297018) (see Reagent Setup)Agarose (Fisher Scientific, cat.

(10) Stress-induced transcriptional readthrough into neighboring genes is linked to intron retentioniScienceDecember 22, 2022Shani Hadar, Anatoly Meller, Naseeb Saida, Reut Shalgi(Promega, #M170A). A 100ng of cDNA was added to a 25ul PCR reaction using Phusion polymerase (NEB, #M0535L) according to manufacturer’s instructions, for 25 cycles. no-RT control samples were generated similarly,

(11) Harmony COVID-19: A ready-to-use kit, low-cost detector, and smartphone app for point-of-care SARS-CoV-2 RNA detectionScience AdvancesDecember 17, 2021Nuttada Panpradist et al.gene downstream of the finger domain was amplified by PCR using Phusion Hot Start Flex DNA Polymerase (M0535L, New England Biolabs) with a modified forward primer containing sequence overlap with the T. in polA

(12) Tumor evolution selectively inactivates the core microRNA machinery for immune evasionNature communicationsDecember 1, 2021Song TY, Long M, Zhao HX, Zou MW, Fan HJ, Liu Y, Geng CL, Song MF, Liu YF, Chen JY, et al.constructed library was amplified by two-step PCRs using Phusion® Hot Start Flex DNA Polymerase (NEB, M0535L), in which the second step of PCR was conducted using the PCR amplicon from the first PCR as template.

(13) Sirt1 promotes tissue regeneration in zebrafish through regulating the mitochondrial unfolded protein responseiScienceOctober 22, 2021Yi-Fan Lin, Jessica Sam, Todd Evansprotein,PNA Bio,CP01-200 T7 nuclease,New England Biolabs,M0302L Pfusion Taq polymerase,New England Biolabs,M0535L ,, Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays ,, mMESSAGE

(14) Augmenting and directing long-range CRISPR-mediated activation in human cellsNature MethodsSeptember 1, 2021Y. Esther Tak, Joy E. Horng, Nicholas T. Perry, Hayley T. Schultz, Sowmya Iyer, Qiuming Yao, Luli S. Zou, Martin J. Aryee, Luca Pinello, J. Keith Joungforward and reverse primer, 200 μM dNTP, 1 unit of Phusion Hot Start Flex DNA Polymerase (NEB, Cat#M0535L) and 1X Phusion HF buffer in a total volume of 50 μl. The first PCR cycling conditions were 98°C for

(15) Linking plasmid-based beta-lactamases to their bacterial hosts using single-cell fusion PCRElifeJuly 20, 2021Peter J Diebold, Felicia N New, Michael Hovan, Michael J Satlin, Ilana L Britoup in duplicate as follows: 25 μl reactions with 20 U/ml Phusion Hot Start Flex DNA polymerase (NEB M0535L), 1× HF Buffer, 200 μM dNTP mix (NEB N0447L), 400 nM of 519F and 786R, 1× SYBR Green (Thermo Fisher

(16) Linking plasmid-based beta-lactamases to their bacterial hosts using single-cell fusion PCReLifeJuly 20, 2021Peter J Diebold, Felicia N New, Michael Hovan, Michael J Satlin, Ilana L Britoup in duplicate as follows: 25 μl reactions with 20 U/ml Phusion Hot Start Flex DNA polymerase (NEB M0535L), 1× HF Buffer, 200 μM dNTP mix (NEB N0447L), 400 nM of 519F and 786R, 1× SYBR Green (Thermo Fisher

(17) Selective multiplexed enrichment for the detection and quantitation of low-fraction DNA variants via low-depth sequencingNature Biomedical EngineeringJuly 1, 2021Ping Song, Sherry X. Chen, Yan Helen Yan, Alessandro Pinto, Lauren Y. Cheng, Peng Dai, Abhijit A. Patel, David Yu Zhangblockers, and then underwent 23 cycles of PCR, using Phusion Hot Start Flex DNA polymerase (NEB, catalog #M0535L). Here, the reaction volume is 50 μL, and the thermocycling protocol including an initial 98 °C denaturation

(18) Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balanceMolecular cellMay 20, 2021Schep R, Brinkman EK, Leemans C, Vergara X, van der Weide RH, Morris B, van Schaik T, Manzo SG, Peric-Hupkes D, van den Berg J, et al.D4551 Shield-1,Aeobius,Cat#: AOB1848 Phusion® Hot Start Flex DNA Polymerase,New England BioLabs,Cat#: M0535L Phusion® HF DNA Polymerase,New England BioLabs,Cat#: M0530L MyTac Red Mix 2x,Bioline,Cat#: BIO-25044 CleanPCR,CleanNA,Cat#:

(19) Phosphorylation of the androgen receptor at Ser81 is co‐sustained by CDK1 and CDK9 and leads to AR‐mediated transactivation in prostate cancerMolecular OncologyMay 3, 2021XinTao Gao et al.nest‐PCR PCR round‐1 and round‐2 (nest‐PCR) were performed using Phusion Hot Start Flex DNA Polymerase (NEB, M0535L). The PCR condition for PCR round 1 was: 98 °C for 2 min; 60× (98 °C for 15 s; 63 °C for 30 s; 72 °C

(20) Eltrombopag Improves Erythroid Differentiation in a Human Induced Pluripotent Stem Cell Model of Diamond Blackfan AnemiaCellsMarch 26, 2021Qanash H, Li Y, Smith RH, Linask K, Young-Baird S, Hakami W, Keyvanfar K, Choy JS, Zou J, Larochelle A.Beckman Coulter Life Sciences, Indianapolis, IN, USA). PCR was then performed with Phusion® Hot Start Flex DNA Polymerase (M0535L, NEB) for 43 cycles at 60 °C in GC buffer with 4 µM SYTO-82 (S1133, Thermo Fisher Scientific). Two primers

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Phusion Hot Start Flex DNA Polymerase

Phusion Hot Start Flex DNA Polymerase from New England Biolabs

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Phusion Hot Start Flex DNA Polymerase from New England Biolabs

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