Bst2.0 WarmStartDNA Polymerase M0538S from New England Biolabs

Product Specs
ItemBst2.0 WarmStartDNA Polymerase
CompanyNew England Biolabs
Price $87.00 Inquire
Catalog NumberM0538S
Size1600 units
ApplicationsDNA Analysis
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A One-Pot CRISPR/Cas12a-Based Platform for Contamination-Free Nucleic Acid Amplification DetectionBiosensors (Basel)March 19, 2026Wei Tantai, Qinfeng Xu, Wenjuan Zhang, Yanni Li, Hao Liuantarctic thermolabile UDG (M0372S), and the LAMP reagents, including Bst 2.0 WarmStart DNA polymerase (M0538S), MgSO4 (B1003S), dNTPs (N0447S), and 10× LAMP amplification buffer (2 mM MgSO4, 10 mM (NH4)2SO4, 50

(2) Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cellsNucleic Acids ResMarch 14, 2026Neesha Kara, Laura Biggins, Alex Whale, Kieron May, Vera Grinkevich, Paola Garran-Garcia, Jhanavi Srinivasan, Peter J Rugg-Gunn, Claudia Ribeiro de Almeida, Samantha J Walker, et al.B0537S), 3 µl 100 mM MgSO4 (NEB, B1003S), 2 µl 10 mM dNTPs, and 1 µl Bst 2 WarmStart DNA polymerase (NEB, M0538S) was added to the sample and incubated 30 min at 65°C before ethanol precipitation with 12.5 µl per

(3) Massively parallel reporter assay for mapping gene-specific regulatory regions at single-nucleotide resolutioneLifeFebruary 25, 2026Alastair J Tulloch, Ryan Nicholas Delgado, Rinaldo Catta-Preta, Constance L Cepko50°C for 1 hr, column-purified, and incubated with Bst 2.0 WarmStart Polymerase (8 units, NEB, cat. #M0538) in 1x Isothermal Amplification Buffer supplemented with 6 mM MgSO₄ and 200 µM dNTPs (GoldBio, cat.

(4) High-Resolution Chloroplast SNV Profiling of 409 Grapevine (Vitis vinifera L.) Cultivars Using Whole-Genome Shotgun SequencingInt J Mol SciFebruary 5, 2026Katarina Rudolf Pilih, Tomaž Kasunič, Tjaša Cesar, Denis Rusjan, Mitra Razi, Tatjana Jovanović-Cvetković, Aida Dervishi, Dragoslav Ivanišević, Katerina Biniari, Klime Beleski, et al.accession using T4 DNA ligase (NEB, cat. no. M0202) and Bst 2.0 WarmStart® DNA polymerase (NEB, cat. No.M0538). All ligated products were pooled and cleaned with magnetic beads (Magtivio, Daelderweg, The Netherlands,

(5) The late arrival of domestic cats in China via the Silk Road after 3,500 years of human-leopard cat commensalismCell GenomicsJanuary 14, 2026Yu Han, Songmei Hu, Ke Liu, Xiao Xu, Ziyi Li, Sean Doherty, Alexandra Elizabeth Jamieson, Aurélie Manin, Sofia Granja Martins, Miaomiao Yang, et al.Polymerase (3 U/μL),NEB,Cat#M0203L Bst 2.0 WarmStart DNA Polymerase (8 U/μL),New England Biolabs,Cat#M0538L Bovine Serum Albumin,Takara Bio,Cat#2320 SPRI Magnetic Beads (AMPure XP),Beckman Coulter,Cat#A63882 PowerUp

(6) Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in Thailand☆MethodsXDecember 1, 2025Wansadaj Jaroenram, Yada Ongchanchai, Ravee Nitiyanontakij, Pawita Suwanwatthana, Ratchana Ponang, Wansika Kiatpathomchaidetection Reagents/tools,"Reagents•Bst 2.0 WarmStart® DNA Polymerase (New England Biolabs, Cat. no M0538; 8000 U mL-1)•dNTPs (Thermo Fisher Scientific™, Cat. no R0191)•UltraPure™ DNase/RNase-Free Distilled Water

(7) Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urineBiosensors and Bioelectronics: XOctober 1, 2025Duc Anh Thai, Jing Liu, Angel Gutierrez Gutierrez Ruiz, Yuqian Zhang, Marina Walther-Antonio, Yuguang Liusolution mix (10 mM each dNTP) and LAMP kit including 8000 U mL−1 Bst 2.0 WarmStart DNA Polymerase (M0538), 10 × isothermal amplification buffer, and 100 mM MgSO4 were purchased from New England Biolabs (Ipswich,

(8) Heterogeneous pericoerulear neurons tune arousal and exploratory behavioursNatureJuly 10, 2025Andrew T. Luskin, Li Li, Xiaonan Fu, Madison M. Martin, Kelsey Barcomb, Kasey S. Girven, Taylor Blackburn, Bailey A. Wells, Sarah T. Thai, Esther M. Li, et al.3.5 μL 10 μM TSO primer, 0.5 μL 20 mg/mL BSA (NEB, B9000), 3 μL BST2.0 WarmStart DNA Polymerase (NEB, M0538), and 49 μL H2O), then the cDNAs eluted into a tube. To introduce UMIs, ~35 μL sample was mixed with

(9) Innovative nucleic acid detection of Clostridioides difficile utilizing the PAM-unconventional, one-step LAMP/CRISPR-Cas12b detection platformsFrontiers in Cellular and Infection MicrobiologyMay 29, 2025Yizhuo Zhang, Luqin Lv, Su Xu, Yijian Chenstandard protocol provided by NEB® (https://www.neb.cn/zh-cn/protocols/2014/12/29/typical-lamp-protocol-m0538) in a final volume of 25 μL. Each reaction included 5 μL of plasmid diluted in salmon sperm DNA solution

(10) Sample-to-answer detection of miRNA from whole blood using thermally responsive alkane partitionsBiosensors and BioelectronicsMarch 1, 2025Evan H. Benke, David J. Boegner, Alejandra M. Bogusch, Ian M. WhiteEngland Biolabs (NEB) and include SplintR Ligase (NEB#M0375), Bst 2.0 WarmStart DNA Plolymerase (NEB#M0538), Antarctic Thermolabile UDG (NEB#M0372), LAMP Fluorescent Dye (NEB#B1700), dNTP Solution Set (NEB#N0446),

(11) Fetal hepatocytes protect the HSPC genome via fetuin-ANatureJanuary 1, 2025Guo XL, Wang YD, Liu YJ, Chu L, Zhu H, Hu Y, Wu RY, Xie HY, Yu J, Li SP, et al.the strand displacement reaction, the eluent was mixed with 10 U of Bst 2.0 WarmStart DNA polymerase (M0538, NEB) in 1× Q5 polymerase reaction buffer and incubated at 65 °C for 30 min. The reaction was then halted

(12) Establishment of Sample-to-Answer Loop-Mediated Isothermal Amplification-Based Nucleic Acid Testing Using the Sampling, Processing, Incubation, Detection and Lateral Flow Immunoassay PlatformsBiosensors (Basel)December 13, 2024Lilas Pommiès, Hervé Boutal, David Fras, Hervé Vollandmagnesium sulfate (MgSO4, catalog # B1003S) solution, and Bst 2.0 Warm Start DNA Polymerase (catalog # M0538L) were from New England Biolabs France (Evry, France). 2.2. Solution Preparation 2.2.1. Conjugate

(13) Global coupling of R-loop dynamics with RNA polymerase II modulates gene expression and early development of DrosophilaNucleic Acids ResearchNovember 27, 2024Xianhong Zhang, Shao-Bo Liang, Zhuoyun Yi, Zhaohui Qiao, Bo Xu, Huichao Geng, Honghong Wang, Xinhua Yin, Mingliang Tang, Wanzhong Ge, et al.eluted in 10 μl 0.1% Tween-20. The eluent was mixed with 10 U Bst 2.0 WarmStart DNA polymerase (NEB, M0538) in 1 × Q5 polymerase reaction buffer, incubated at 65°C for 30 min, and stopped by incubation at 80°C

(14) Optimization of loop-mediated isothermal amplification assay for sunflower mildew disease detectionScientific ReportsOctober 5, 2024Oğuzhan Yeni, Mutlu Şen, Semra Hasançebi, Neslihan Turgut Karastudies were conducted using Bst 2.0 Warm Start DNA Polymerase (8,000 U/ml) (NEW ENGLAND BIOLABS, Cat no: M0538) in a thermal cycler (APPLIED BIOSYSTEM, SimpliAmp) and a real-time thermal cycler device (BIO-RAD,

(15) Development of a loop-mediated isothermal amplification detection assay for Dictyocaulus viviparus (Bloch, 1782) lungworm: DviLAMPFrontiers in Veterinary ScienceOctober 4, 2024Sirapat Nak-on, Paul Campbell, Maha Mansour Shalaby, Jennifer McIntyre, Alistair Antonopoulos, Thapana Chontananarth, Roz Laing0.75 μL of 100 mM MgSO4, 2.5 μL of 5 M Betaine, 0.5 μL of 8 U/μL Bst 2.0 WarmStart DNA Polymerase (M0538S, NEB), 0.25 μL of 50X LAMP Fluorescent Dye (B1700S, NEB), 0.375 μL of 1 μM passive reference dye (600530,

(16) Comparison and Optimization of Simple DNA Extraction Methods for LAMP-Based Point-of-Care Applications Employing Submillimeter Skin BiopsiesACS OmegaSeptember 17, 2024Juan M. Boza, Jason Cade Manning, David C. EricksonB0537S), 100 mM MgSO4 (NEB B1003S), 10 mM dNTPs (NEB N0447L), and BST 2.0 WarmStart polymerase (NEB M0538S), which were purchased from New England Biolabs. 20× EvaGreen (89138–984) was obtained from VWR International.

(17) Two‐Stage Mixed‐Dye‐Based Isothermal Amplification with Ribonuclease‐Cleavable Enhanced Probes for Dual‐Visualization Detection of SARS‐CoV‐2 Variants of InterestAdvanced ScienceAugust 1, 2024Xiong Ding, Yaru Wang, Yuxin Gui, Chuankun Yang(Supporting Information). MgSO4 (Cat# B1003S), dNTPs (Cat# N0447L), Bst 2.0 WarmStar DNA polymerase (Cat# M0538L), WarmStart RTx (Cat# M0380S), ET SSB (Cat# M2401S), and nuclease‐free water (Cat# B1500S) were purchased

(18) Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)Sci RepMarch 11, 2024May Khat Nwe, Nisachon Jangpromma, Lapatrada Taemaitreeof LAMP was 12.5 µL which contains 0.64 U/µL Bst 2.0 WarmStart® DNA polymerase (8 U/µL, 1 µL, NEB, M0538S), CYTB-FIP and CYTB-BIP (20 µM, 1.125 µL), CYTB-F3 and CYTB-B3 (10 µM, 0.25 µL), CYTB-LF and CYTB-LB

(19) Prevalence of S. aureus and/or MRSA in hospitalized patients with diabetic foot and establishment of LAMP methods for rapid detection of the SCCmec geneBMC MicrobiologyJanuary 26, 2024Chunxia Qi, Xiangrong Luo, Jiali Huang, Danli Kong, Yali Zhang, Mengchen Zou, Hao ZhouBIP, 6 mM MgSO4, 1.4 mM dNTP (CoWin Biosciences, CW0941), 8 U Bst 2.0 Warmstart DNA polymerase (NEB, M0538S), 1 × isothermal amplification buffer, 0.2 M betaine (Sigma, B0300), and 120 µM hydroxynaphthol blue

(20) Simultaneous and rapid colorimetric detection of distinct miRNAs using Split-LAMPFrontiers in Bioengineering and BiotechnologyNovember 2, 2023Yi Jing Chua, Steven Poh Chuen Sim, Medha Shridharan, Yiqi Seow2.2 Primers (Integrated DNA Technologies),As stated in Section 2.2 "WarmStart Bst 2.0 (catalog number M0538M, New England Biolabs)",6.4 U Components in a LAMP reaction.

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Bst2.0 WarmStartDNA Polymerase

Bst2.0 WarmStartDNA Polymerase from New England Biolabs

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Bst2.0 WarmStartDNA Polymerase from New England Biolabs

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