Exonuclease V (RecBCD) M0345L from New England Biolabs

Product Specs
ItemExonuclease V (RecBCD)
CompanyNew England Biolabs
Price $328.00 Inquire
Catalog NumberM0345L
Size5000 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dynamic activation of rAAV transgene expression by a small molecule that recruits endogenous transcriptional machineryNucleic Acids ResApril 29, 2025Sara R Wasserman, Savannah Muron, Hae Rim Lee, Madison L Routh, Austin J Hepperla, Deena M Scoville, Avery Huber, Jessica D Umana, Nicole E Pereira, Caroline A Foley, et al.digested with AvrII (NEB R0174S) for 3 h followed by digestion with plasmid-safe Exonuclease V (NEB M0345L) for 10 h. Viral episomal copy number was then quantified by qPCR amplification of Exonuclease V digestion-resistant

(2) EccDNA atlas in male mice reveals features protecting genes against transcription-induced eccDNA formationNat CommunFebruary 22, 2025Xue Liang, Gerard Arrey, Yating Qin, Lucía Álvarez-González, Judith Mary Hariprakash, Jie Ma, Sylvester Holt, Peng Han, Yonglun Luo, Hanbo Li, et al.Each tube started the digestion with 30 U of exonuclease V (RecBCD) (New England Biolabs, Cat. No. M0345L), together with NEBuffer 4 and ATP, according to the RecBCD recommendations. The samples were incubated

(3) Variation of extrachromosomal circular DNA in cancer cell linesComputational and Structural Biotechnology JournalAugust 28, 2023Carl Rung dos Santos, Lasse Bøllehuus Hansen, Monica Rojas-Triana, Astrid Zedlitz Johansen, Mirna Perez-Moreno, Birgitte RegenbergDNA, 40 µg total DNA was mixed with 10 µl NEBuffer 4 (10X), 3 µl Exonuclease V (10,000 U/ml) (cat.no. M0345L, NEB), 10 µl ATP (10 mM), and nuclease-free H2O to reach a final volume of 100 µl. This was incubated

(4) Accurate profiling of full-length Fv in highly homologous antibody libraries using UMI tagged short readsNucleic Acids ResearchJune 23, 2023Itay Levin, Marek Štrajbl, Yair Fastman, Dror Baran, Shir Twito, Jessica Mioduser, Adi Keren, Sharon Fischman, Michael Zhenin, Guy Nimrod, et al.heat inactivation, undesired linear fragments were removed by addition of 2 μl (20U) ExoV (NEB cat:M0345L) and 10 μl of 10 mM ATP, and incubation at 37°C for 30 min followed by heat inactivation at 70°C for

(5) Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus librariesNat CommunNovember 16, 2022Arpiar Saunders, Kee Wui Huang, Cassandra Vondrak, Christina Hughes, Karina Smolyar, Harsha Sen, Adrienne C. Philson, James Nemesh, Alec Wysoker, Seva Kashin, et al.Step 3 = Circular plasmid enrichment (spike-in, +8.8 μl): (1) Exonuclease V (RecBCD, 10,000 U/ml, NEB M0345L), 1 μl; (2) NEBuffer 4 (10x, NEB B7004S), 1.4 μl; (3) ATP (10 mM, NEB P0756L), 6.4 μl. Step 3 Cycle

(6) Targeted removal of mitochondrial DNA from mouse and human extrachromosomal circular DNA with CRISPR-Cas9Computational and Structural Biotechnology JournalJune 15, 2022Weijia Feng, Gerard Arrey, Egija Zole, Wei lv, Xue Liang, Peng Han, Marghoob Mohiyuddin, Henriette Pilegaard, Birgitte RegenbergDNA (1–4 μg) were used for linear DNA removal. Linear DNA was removed by ExonucleaseV digestion (NEB, M0345L, USA) for 7 days at 37 °C, adding additional ATP and DNase every day (25 U/day). Afterwards, exonuclease

(7) Circular DNA in the human germline and its association with recombinationMolecular CellJanuary 1, 2022Rasmus Amund Henriksen, Piroon Jenjaroenpun, Ida Borup Sjøstrøm, Kristian Reveles Jensen, Iñigo Prada-Luengo, Thidathip Wongsurawat, Intawat Nookaew, Birgitte Regenbergpeptides, and recombinant proteins" ,, exonuclease V RecBCD,New England Biolabs (BioNordika Denmark),NEB-M0345L proteinase K,ThermoFisher,#EO0491 ,, Critical commercial assays,Critical commercial assays,Critical

(8) Unbiased PCR-free spatio-temporal mapping of the mtDNA mutation spectrum reveals brain region-specific responses to replication instabilityBMC BiologyDecember 1, 2020Emilie Kristine Bagge, Noriko Fujimori-Tonou, Mie Kubota-Sakashita, Takaoki Kasahara, Tadafumi Katoat 37 °C with interval shaking (1000 rpm) (18 μL total DNA, 3 μL NEBuffer 4 (10x), 4 μL ExoV (NEB, #M0345L), 6 μL ATP (10 mM)). Additional 1 μL NEBuffer 4 (10x), 2 μL ExoV, 6 μL ATP (10 mM) was added and incubated

(9) HEx: A heterologous expression platform for the discovery of fungal natural productsScience AdvancesApril 11, 2018Colin J. B. Harvey et al.volume of 22.5 ml of each prepared plasmid was combined with 1.5 ml of Exonuclease V (10 U/ml; NEB, M0345L), 3.0 ml of NEB Buffer 4 (NEB, B7004S), and 3.0 ml of ATP (10 mM; NEB, P0756S) followed by incubation

(10) A peptide tag-specific nanobody enables high-quality labeling for dSTORM imagingNature communicationsMarch 2, 2018Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhöfer M, Schmees C, Vojnovic I, Pisak-Lukáts B, Endesfelder U, Rothbauer U.(New England Biolabs, cat. #E5520S). After treatment with Exonuclease RecBCD (New England Biolabs, cat. # M0345L) the column purified DNA plasmid was digested over night at 37 °C with BbsI. The linearized plasmid

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Exonuclease V (RecBCD)

Exonuclease V (RecBCD) from New England Biolabs

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Exonuclease V (RecBCD) from New England Biolabs

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