T7 DNA Ligase M0318L from New England Biolabs

Product Specs
ItemT7 DNA Ligase
CompanyNew England Biolabs
Price $301.00 Inquire
Catalog NumberM0318L
Size750000 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) CRISPR-based functional genomics for dissecting therapeutic dependency in primary acute myeloid leukemia samplesMolecular CellMarch 5, 2026Zhendong Cao, Sixiang Yu, Jacqueline Peng, Declan R. Barrett, Yuqiao Liu, Jonathan H. Sussman, Changya Chen, Anusha Thadi, Li Liu, Fatemeh Alikarami, et al.cytokines for human cells,STEMCELL Technology,Cat # H4434 T4 DNA ligase,NEB,M0202L T7 DNA ligase,NEB,M0318L PEG-8000,Sigma-Aldrich,P2139 Busulfan,Meitheal,NDC 71288–116-11 sparQ PureMag Beads,Quantabio/VWR,95196–060 KAPA HiFi HotStart ReadyMix

(2) One-pot cloning and protein expression platform for genetic engineeringbioRxivSeptember 11, 2025Wakana Sato, Judee Sharon, Brock Cash, Christopher Deich, Nathaniel J. Gaut, Joseph Heili, Aaron E. Engelhart, Katarzyna P. Adamala25 ng/μl of PCR product, 1.55 units/μl of BsaI-HF®v2 (NEB, R3733L), 150 units/μl of T7 ligase (NEB, M0318L), 1mM ATP, 1x CutSmart® Buffer (NEB, B7204S). The incubation was performed on the thermal cycler: Initial

(3) Real-Time Imaging of Specific Genomic Loci With CRISPR/dCas9 in Human Cells Using CRISPRainbowBIO-PROTOCOLSeptember 5, 2025Thomas J. Versosky, Dilshodbek U. Nishonov, Li-Chun Tu(New England Biolabs, catalog number: R3539L), storage: -20 °C 4. T7 DNA Ligase (New England Biolabs, catalog number: M0318L), storage: -20 °C 5. CutSmartTM buffer, 10× (New England Biolabs, catalog number: B6004S), storage:

(4) Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxNat CommunJuly 29, 2025Megan D. Schertzer, Andrew Stirn, Keren Isaev, Laura Pereira, Stella H. Park, Anjali Das, Aline Réal, Erin D. Jeffery, Claire Harbison, Hans-Hermann Wessels, et al.were mixed with 25 ng digested plasmid, 0.25 µl T7 ligase, and 5 µl 2x T7 Stick together buffer (NEB M0318L). The ligation reaction was incubated at 25 °C for 20 min and transformed into competent cells. Guide

(5) Systemic genome-epigenome analysis captures a lineage-specific super-enhancer for MYB in gastrointestinal adenocarcinomaMolecular Systems BiologyApril 15, 2025Fuyuan Li, Shangzi Wang, Lian Chen, Ning Jiang, Xingdong Chen, Jin Li,New England Biolabs,M0201L T4 DNA Ligase Reaction Buffer,New England Biolabs,B0202S T7 DNA ligase,New England Biolabs,M0318L Luna® Universal qPCR Master Mix,New England Biolabs,M3003E LunaScript RT SuperMix,New England Biolabs,M3010L Q5®

(6) A CRISPR activation screen identifies FBXO22 supporting targeted protein degradationNature chemical biologyDecember 1, 2024Basu AA, Zhang C, Riha IA, Magassa A, Campos MA, Caldwell AG, Ko F, Zhang X.in the presence of Esp3I (Thermo Scientific, cat# ER0452) and T7 ligase (New England Biolabs, cat# M0318L). The construct library was transformed into ElectroMAX Stbl4 competent cells (Thermo Scientific, cat#

(7) Effect of genomic and cellular environments on gene expression noiseGenome BiologyMay 24, 2024Clarice K. Y. Hong, Avinash Ramu, Siqi Zhao, Barak A. Cohennuclease-free H2O to a concentration of 400 uM. The three arms were ligated by T7 ligase (NEB, Cat# M0318L) at 25 °C overnight, then purified using the Monarch PCR and DNA cleanup Kit (NEB, Cat# T1030S), and

(8) Regulation of T helper cell differentiation by the interplay between histone modification and chromatin interactionImmunityMay 14, 2024Shuai Liu, Yaqiang Cao, Kairong Cui, Gang Ren, Tingting Zhao, Xuezheng Wang, Danping Wei, Zuojia Chen, Rama Krishna Gurram, Chengyu Liu, et al.,New England Biolabs,Cat# M0212L T4 DNA Ligase,New England Biolabs,Cat# M0202L T7 DNA Ligase,New England Biolabs,Cat# M0318L Dynabeads MyOne Streptavidin C1 beads,Invitrogen,Cat# 65001 Dynabeads Protein A for Immunoprecipitation,Invitrogen,Cat#

(9) Multifaceted roles of cohesin in regulating transcriptional loopsbioRxivMarch 28, 2024Minji Kim, Ping Wang, Patricia A. Clow, I (Eli) Chien, Xiaotao Wang, Jianhao Peng, Haoxi Chai, Xiyuan Liu, Byoungkoo Lee, Chew Yee Ngan, et al.R0137L Proteinase K,ThermoFisher,Cat# 25530049 T4 Polynucleotide Kinase,NEB,Cat# M0201L T7 DNA ligase,NEB,Cat# M0318L 3-indoleacetic acid,Sigma Aldrich,Cat# I3750-25G-A Lipofectamine LTX,ThermoFisher Invitrogen,Cat#

(10) Temporally resolved transcriptional recordings into E. coli DNA using a Retro-CascorderNature ProtocolsJune 1, 2023Sierra K. Lear, Santiago C. Lopez, Alejandro González-Delgado, Santi Bhattarai-Kline, Seth L. Shipmanbuffer. Refer to Supplementary Methods for details. Q5 High-Fidelity DNA Polymerase (NEB, cat. no. M0318L) CRITICAL Use of a high-fidelity polymerase minimizes errors in amplification of high-diversity libraries

(11) Hi-TrAC reveals division of labor of transcription factors in organizing chromatin loopsNature communicationsNovember 5, 2022Liu S, Cao Y, Cui K, Tang Q, Zhao K.were ligated to multiplexing adapters by adding 5 μL each adapter (50 μM) and 1 μL T7 DNA ligase (NEB, M0318L) in 100 μL ligation mixture and incubating at room temperature for 1 h with rotation. Before PCR amplification,

(12) ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situNature MethodsNovember 1, 2022Ian Dardani, Benjamin L. Emert, Yogesh Goyal, Connie L. Jiang, Amanpreet Kaur, Jasmine Lee, Sara H. Rouhanifard, Gretchen M. Alicea, Mitchell E. Fane, Min Xiao, et al.probe can bind), and a universal 10mer sequence (CTGAGTGTTG). Then, T7 DNA Ligase (New England Biolabs, M0318L) was added to ligate a complete 109mer (50 + 1 + 58) primary probe. We then added ammonium acetate to

(13) TALEN outperforms Cas9 in editing heterochromatin target sitesNature CommunicationsDecember 1, 2021Surbhi Jain et al.site with PAM were phosphorylated and annealed and then ligated to the backbone using T7 Ligase (NEB # M0318L). 0.5×10^5 cells/well were plated in a 24-well plate, 24 h before transfection. 335 ng reporter plasmid

(14) Darwinian properties and their trade-offs in autocatalytic RNA reaction networksNature CommunicationsDecember 1, 2021Sandeep Ameta, Simon Arsène, Sophie Foulon, Baptiste Saudemont, Bryce E. Clifton, Andrew D. Griffiths, Philippe NgheOligo 13, bottom, Supplementary Data 1), 30 U/µL of T7 DNA Ligase (New England Biolabs, Product No.: M0318L), at room temperature for 25 min with agitation at 600 rpm. Beads were then washed five times with 4

(15) Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic productsMicrobial Cell FactoriesDecember 1, 2020Arun S. Rajkumar, John P. Morrisseyclone the genes into storage plasmids (level I) by Golden Gate assembly with BsmBI and T7 DNA ligase (M0318L, NEB) for subsequent assembly into expression cassette-bearing plasmids (level II) using the hierarchical

(16) Constitutively bound CTCF sites maintain 3D chromatin architecture and long-range epigenetically regulated domainsNature CommunicationsDecember 1, 2020Amanda Khoury et al.1 μl Fast Digest BbsI (#FD1014, Thermo Fisher) and 0.5 μl T7 ligase with 2x rapid ligation buffer (#M0318L, NEB). The reaction was incubated for a total of 60 min in a thermocycler on the following cycle: 37

(17) HDAC1 inhibition ameliorates TDP-43-induced cell death in vitro and in vivoCell death & diseaseMay 14, 2020Sanna S, Esposito S, Masala A, Sini P, Nieddu G, Galioto M, Fais M, Iaccarino C, Cestra G, Crosio C.harboring a BbsI overhang. Afterwards, BbsI (#FD1014, ThermoFisher) mediated digestion and T4 DNA ligase (#M0318L, NEB) directed ligation in the linearized pSpCas9(BB)-2A-Puro was performed. After the transformation,

(18) Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloideseLifeMarch 9, 2018Samuel T Coradetti et al.10x T4 ligase buffer, 5 ml 10x NEB CutSmart buffer (NEB, B7204S, Ipswich, MA), 2.5 ml T7 ligase (NEB, M0318L), and 2.5 ml of SapI (NEB, R0569S). We incubated the reaction at 37˚C for 5 min, then 25 cycles of 37˚C

(19) CRISPR/Cas9-mediated knockout of p22phox leads to loss of Nox1 and Nox4, but not Nox5 activityRedox biologyOctober 1, 2016Prior KK, Leisegang MS, Josipovic I, Löwe O, Shah AM, Weissmann N, Schröder K, Brandes RP.GGC CGA ACC CCG C-3′. Afterwards, BbsI (#FD1014, ThermoFisher) mediated digestion and T7 DNA ligase (#M0318L, NEB) directed ligation was performed followed by PlasmidSafe exonuclease treatment (#E3101K, Epicenter/Biozym)

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T7 DNA Ligase

T7 DNA Ligase from New England Biolabs

New England Biolabs

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T7 DNA Ligase from New England Biolabs

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