mRNA Cap 2-O-Methyltransferase M0366S from New England Biolabs

Product Specs
ItemmRNA Cap 2-O-Methyltransferase
CompanyNew England Biolabs
Price $61.00 Inquire
Catalog NumberM0366S
Size2000 units
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Robust CD8+ T cell responses induced by an mRNA-LNP vaccine encoding rat HER2 extracellular domain confer prophylactic tumor protectionFront ImmunolApril 1, 2026Kexin Li, Xiaoya Li, Cong Zhang, Suli Dai, Lei Li, Jun Wang, Hongtao Zhang, Lianmei Zhaothe m7G capping kit (#M2080S, New England Biolabs, USA) in conjunction with 2’-O-methyltransferase (#M0366S, New England Biolabs, USA) to form Cap1 structures. Polyadenylation was performed using E. coli Poly(A) Polymerase

(2) Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growthbioRxivMarch 14, 2026Lucille H. Tsao, Doug E. Brackney, Anna Marie Pyleusing the Vaccinia capping Enzyme (NEB, cat #M2080S) and mRNA Cap 2’-O-Methyltransferase (NEB, cat #M0366S) according to manufacturer’s protocols. The capping protocol added a type-1 cap with an inverted 7-methylguanosine

(3) A ribosome-bound pseudoknot in the HCV coding region stimulates viral growth by tuning viral translationCell ReportsJanuary 27, 2026Han Wan, Harim Jang, Ling Xu, Kyrillos S. Abdallah, Wendy V. Gilbert, Anna Marie Pyleinitiation, the RNA was capped using Vaccinia Capping Enzyme (NEB M2080S) and 2′-OMe transferase (NEB M0366S) following the manufacturer’s protocols. After capping, the RNA was purified again using the RNeasy

(4) A novel plasmid-based co-tethered transcription platform for high yield, high purity mRNA synthesisNucleic Acids ResDecember 11, 2025Purnima Mala, Ruptanu Banerjee, Amin Abek, James Forster Iii, Aniruddha Pinjari, Ashish A Kulkarni, Craig T MartinmM SAM, 10 U vaccinia capping enzyme, and 50 U mRNA cap 2’-O-Methyltransferase (New England Biolabs, M0366S) and incubated at 37°C for 30 min in 20 μl volume. RNA is ready for use in in vitro cell-based assays

(5) Systematic screening of excipients to stabilize aerosolized lipid nanoparticles for enhanced mRNA delivery†RSC PharmaceuticsSeptember 16, 2025Brittany J. Heiser, Mae M. Lewis, Meysam Mohammadi Zerankeshi, Emily K. Netemeyer, Ashlee M. Hernandez, Alexander E. Marras, Debadyuti GhoshVaccinia Capping System (New England Biolabs, M2080S) and mRNA Cap 2′-O-methyltransferase (New England Biolabs, M0366S). A 3′-poly(A) tail approximately 100 bp long was added using E. coli poly (A) polymerase (New England Biolabs

(6) Engineered RNA-based activation system for coronavirus sensing in live cellsBioDesign ResearchSeptember 1, 2025Leiping Zeng, Christian Otero, Lei S. Qipositive sense RNAs were capped with vaccinal capping enzyme (NEB #M2080S) and methyltransferase (NEB# M0366S) by following the vendor's manuscripts. 2.4 Transient transfection and VERAS activity tests 293T/hAPN

(7) A two-step mechanism for RIG-I activation by influenza virus mvRNAsSci AdvAugust 8, 2025Emmanuelle Pitré, Karishma Bisht, Kaleigh A. Remick, Amir Ghorbani, Jonathan W. Yewdell, Elizaveta Elshina, Aartjan J.W. Te VelthuisU of vaccina virus capping system (NEB, M2080S), and 25 U of mRNA Cap 2′ O-methyltransferase (NEB, M0366S) for 1 hour at 37°C and for 4 min at 70°C. Unincorporated radiolabel was removed with the Oligo Clean & Concentrator

(8) A Bivalent mRNA Vaccine Efficiently Prevents Gammaherpesvirus Latent InfectionVaccines (Basel)August 4, 2025Yannan Yin, Jinkai Zang, Huichun Shi, Zhuang Wang, Linlin Kuang, Shuxia Wang, Haikun Wang, Ning Li, Xiaozhen Liang, Zhong Huang(New England BioLabs, Ipswich, MA, USA, M2080S) and mRNA Cap 2′ -OMethyltransferase (New England BioLabs, M0366S) to produce the Cap I structure. The capped mRNAs were purified and stored at −80 °C until use. LNP

(9) Trans amplifying mRNA vaccine expressing consensus spike elicits broad neutralization of SARS CoV 2 variantsNPJ VaccinesJune 3, 2025Abhinay Gontu, Sougat Misra, Shubhada K. Chothe, Santhamani Ramasamy, Padmaja Jakka, Maurice Byukusenge, Lindsey C. LaBella, Meera Surendran Nair, Bhushan M. Jayarao, Marco Archetti, et al.capped using the Vaccinia Capping System (Cat.# M2080S) and mRNA Cap 2’-O-Methyltransferase (Cat.# M0366S) from NEB, USA. The capped RNA was subsequently purified with the Monarch® RNA Cleanup Kit (Cat.# T2040L)

(10) Engineered circular RNA-based DLL3-targeted CAR-T therapy for small cell lung cancerExp Hematol OncolMarch 12, 2025Jingsheng Cai, Zheng Liu, Shaoyi Chen, Jingwei Zhang, Haoran Li, Xun Wang, Feng Yang, Shaodong Wang, Xiao Li, Yun Li, et al.followed by capping and polyadenylation reactions. For capping, mRNA cap 2′-O-methyltransferase (NEB, M0366S) and Vaccinia capping enzyme (NEB, M2080S) were used according to the manufacturer’s instructions. PolyA

(11) Optimizing Human Cell-Free System for Efficient Protein ProductionJournal of Microbiology and BiotechnologyFebruary 25, 2025Abbas Mansour, Kalle Kipper, Arto PulkMethylation Vaccinia capping system (NEB, M2080S) and mRNA Cap 2'-O-Methyltransferase MTase (NEB, M0366S) were used to add the cap1 structure to the mRNA. The capping reaction was set up in 100 μl. First,

(12) Safety and immunogenicity of an optimized self-replicating RNA platform for low dose or single dose vaccine applications: a randomized, open label Phase I study in healthy volunteersNature communicationsJanuary 7, 2025Maine CJ, Miyake-Stoner SJ, Spasova DS, Picarda G, Chou AC, Brand ED, Olesiuk MD, Domingo CC, Little HJ, Goodman TT, et al.capped using Vaccinia Capping System (NEB, Cat# M2080S) and mRNA Cap 2′-O-Methyltransferase (NEB, Cat# M0366S) and purified by LiCl precipitation. For the clinical study, the GMP srRNA drug substance was produced

(13) The West Nile virus genome harbors essential riboregulatory elements with conserved and host-specific functional rolesProceedings of the National Academy of SciencesJuly 16, 2024Nicholas C. Huston, Lucille H. Tsao, Doug E. Brackney, Anna Marie PyleRNA using the Vaccinia Capping Enzyme (NEB, Cat. No. M2080S) and 2’-OMe transferase (NEB, Cat. No. M0366S) according to the manufacturer’s protocols. Capped RNA was purified using an RNeasy column and eluted

(14) A mRNA Vaccine for Crimean–Congo Hemorrhagic Fever Virus Expressing Non-Fusion GnGc Using NSm Linker Elicits Unexpected Immune Responses in MiceVirusesFebruary 28, 2024Tong Chen, Zhe Ding, Xuejie Li, Yingwen Li, Jiaming Lan, Gary WongBeijing, China), pseudouridine (ψ) (N-1019, TriLink, Shenzhen, China), mRNA Cap 2′-O-Methyltransferase (M0366S, NEB, Beijing, China), DNase I (M0303S, NEB, Beijing, China), and Monarch® RNA Cleanup Kit (T2050L,

(15) Selective Characterization of mRNA 5′ End-Capping by DNA Probe-Directed Enrichment with Site-Specific EndoribonucleasesACS Pharmacology & Translational ScienceNovember 10, 2023Eric J. Wolf, Nan Dai, Siu-Hong Chan, Ivan R. Corrêausing a Faustovirus RNA capping enzyme (FCE; NEB # M2081S) and mRNA cap 2′-O-methyltransferase (NEB # M0366S) according to the manufacturer’s instructions. A substantially low FCE concentration was used to generate

(16) Optimization of ionizable lipids for aerosolizable mRNA lipid nanoparticlesBioengineering & Translational MedicineNovember 1, 2023Mae M. Lewis, Melissa R. Soto, Esther Y. Maier, Steven D. Wulfe, Sandy Bakheet, Hannah Obregon, Debadyuti Ghoshwas added using the Vaccinia Capping System (NEB, M2080S) and mRNA Cap 2′‐O‐methyltransferase (NEB, M0366S). A 3′‐poly(A) tail approximately 100 bp long was then added enzymatically using E. Coli Poly (A) Polymerase

(17) A systems-level mass spectrometry-based technique for accurate and sensitive quantification of the RNA cap epitranscriptomeNature ProtocolsSeptember 1, 2023Jin Wang, Bing Liang Alvin Chew, Yong Lai, Hongping Dong, Luang Xu, Yu Liu, Xin-Yuan Fu, Zhenguo Lin, Pei-Yong Shi, Timothy K. Lu, et al.Vaccinia Capping Enzyme (VCE; NEB, cat. no. M2080S) Vaccinia mRNA 2’-O-methyltransferase (NEB, cat. no. M0366S) Dibutylammonium acetate solution (DBAA; Sigma, cat. no. 73345) Ammonium bicarbonate (Sigma, cat.

(18) Stimulation of interferon-β responses by aberrant SARS-CoV-2 small viral RNAs acting as retinoic acid-inducible gene-I agonistsiScienceJanuary 20, 2023Arai Y, Yamanaka I, Okamoto T, Isobe A, Nakai N, Kamimura N, Suzuki T, Daidoji T, Ono T, Nakaya T, et al.1060A Ribo m7G Cap Analog,PROMEGA,Cat# P171A mRNA Cap 2′-O-Methyltransferase,New England Biolabs,Cat# M0366S Dynabeads™ Protein G for Immunoprecipitation,Thermo Fisher Scientific,Cat# 10003D ExoQuick-TC,System

(19) Nsp16 shields SARS-CoV-2 from efficient MDA5 sensing and IFIT1-mediated restrictionEMBO reportsDecember 6, 2022Russ A, Wittmann S, Tsukamoto Y, Herrmann A, Deutschmann J, Lagisquet J, Ensser A, Kato H, Gramberg T.methyltransferase activity assay. Methyltransferase activity assay Methyltransferases (2.5 U/μl VACV VP39 (NEB, M0366S) or 1.7 μM Nsp16 and 0.3 μM Nsp10 from SARS–CoV‐2) were incubated together with RNA (70 ng/μl mRNA from

(20) Evolution of naturally arising SARS-CoV-2 defective interfering particlesCommunications biologyOctober 27, 2022Girgis S, Xu Z, Oikonomopoulos S, Fedorova AD, Tchesnokov EP, Gordon CJ, Schmeing TM, Götte M, Sonenberg N, Baranov PV, et al.one-step reaction using Vaccinia Capping System (NEB, # M2080S) and mRNA Cap 2′-O-Methyltransferase (NEB, #M0366S). RNA cleanup was performed via phenol-chloroform extraction as described above for cleanup of linearized

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mRNA Cap 2-O-Methyltransferase

mRNA Cap 2-O-Methyltransferase from New England Biolabs

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mRNA Cap 2-O-Methyltransferase from New England Biolabs

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