Exonuclease V (RecBCD) M0345S from New England Biolabs

Product Specs
ItemExonuclease V (RecBCD)
CompanyNew England Biolabs
Price $81.00 Inquire
Catalog NumberM0345S
Size1000 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Global Evaluation of Congenital Heart Disease-Associated Non-Coding VariantsResearch SquareJanuary 7, 2026Edwin G. Rodríguez-Martínez, Shreya Peña-Martínez, Joshua G. Medina-Feliciano, Joshua Medina-Feliciano, Lois G. Parks, Marissa Granitto, Diego A. Pomales-Matos, Jean L. Pomales-Matos, Jean Messon- Barreiro-Rosario, Adriana Barreiro-Rosario, et al.at 50 °C for 1.5 h and purified at 1.5X SPRI. The resulting library was re-digested by RecBCD (NEB, #M0345S) and AsiSI (NEB, #R063L), purified at 1.5X SPRI, and then transformed into E. coli through electroporation

(2) Extrachromosomal circular DNA expressing miRNA promotes ovarian cancer progressionClinical and Translational MedicineSeptember 23, 2025Ning Wu, Ling Wei, Qiyu Liu, Tianhui He, Cuiyu Huang, Yunpeng Jiang, Kailong Li, Hongyan Guo, Fengbiao Mao, Xiaolu Zhao20 min) with 10 cycles. The residual linear DNA was removed by Exonuclease V (New England Biolabs, M0345S), and the LAMA reaction products were purified by VAHTS DNA Clean Beads (Vazyme, N411‐02). 67 Then

(3) Excised DNA circles from V(D)J recombination promote relapsed leukaemiaNatureSeptember 18, 2025Zeqian Gao, James N. F. Scott, Matthew P. Edwards, Dylan Casey, Xiaoling Wang, Andrew D. Gillen, Sarra Ryan, Lisa J. Russell, Anthony V. Moorman, Ruth de Tute, et al.treatment of genomic DNA Linear DNA was removed from genomic DNA using Exonuclease V (RecBCD, NEB M0345S). Reactions comprised 1 µg genomic DNA, 1× NEBuffer 4, 1 mM ATP and 10 U RecBCD in 100 µl total volume.

(4) Identification of a robust promoter in mouse and human hepatocytes by in vivo biopanning of a barcoded AAV libraryMolecular TherapyAugust 6, 2025Jonas Becker, Claire Domenger, Pervinder Choksi, Chiara Krämer, Conradin Baumgartl, Olena Maiakovska, Jae-Jun Kim, Jonas Weinmann, Georg Huber, Florian Schmidt, et al.the AAV-delivered episomal DNA, 1 μg extracted liver DNA was digested with exonuclease V (catalog no. M0345S, NEB) to enrich circular episomes before mixing with control DNA and sonication to an expected fragment

(5) Plasma cell-free extrachromosomal circular DNA is a molecular hallmark of preeclampsiaComputational and Structural Biotechnology JournalJune 6, 2025Peng Han, Chunxiao Zhang, Fangfang Ge, Chengxun Liu, Jing Fu, Wei Lv, Xin Jin, Jianguo Zhang, Lijian Zhao, Yonglun Luo, et al.instructions. 2.3.2 Linear DNA removal and eccDNA amplification Total cfDNA was digested with exonuclease V (RecBCD, M0345S; New England Biolabs, Ipswich, MA, USA) at 37°C for 24 h to remove linear DNA. Exonuclease digestion

(6) Laboratory evolution of the bacterial genome structure through insertion sequence activationNucleic Acids ResMay 14, 2025Yuki Kanai, Atsushi Shibai, Naomi Yokoi, Saburo Tsuru, Chikara FurusawaOne PCR Master Mix Blue (Toyobo, Japan, KMM-201), exonuclease V (RecBCD) (New England Biolabs, USA, M0345S), Rapid Barcoding Kit 96 (Oxford Nanopore Technologies, UK, SQK-RBK110.96), Native Barcoding Kit 24

(7) Characterization and identification of extrachromosomal circular DNA in cholangiocarcinomaPLoS OneMay 5, 2025Zar Zar Win, Hasaya Dokduang, Siriyakorn Kulwong, Watcharin Loilome, Nisana Namwat, Jutarop Phetcharaburanin, Thidathip Wongsurawat, Piroon Jenjaroenpun, Poramate Klanrit, Arporn WangwiwatsineccDNAs in our samples, enzymatic digestion of genomic DNA were employed using exonuclease V RecBCD (NEB, M0345S, USA). Additional fresh reaction buffer, ATP, and exonuclease V were added every 24h. After 48 h digestion,

(8) Mitochondrial Respiratory Dysfunction Is Not Correlated With Mitochondrial Genotype in Premature Aging MiceAging CellMay 2, 2025Hiroaki Tamashiro, Kaori Ishikawa, Koichi Sadotomo, Emi Ogasawara, Kazuto Nakadaprevious report (Bagge et al. 2020). The restriction enzyme reaction solution consisted of 2 μL of Exo V (#M0345S, NEB), 10 μL of NEB buffer 4 (10×), and 10 μL of ATP (10 mM). Approximately 5000 ng of total DNA was

(9) Somatic mosaicism in schizophrenia brains reveals prenatal mutational processesScienceOctober 11, 2024Eduardo A. Maury, Attila Jones, Vladimir Seplyarskiy, Thanh Thanh L. Nguyen, Chaggai Rosenbluh, Taejong Bae, Yifan Wang, Alexej Abyzov, Sattar Khoshkhoo, Yasmine Chahine, et al.was then digested again to remove any uncut plasmids with 50 units of AsiSI, 5 units of RecBCD (NEB, M0345S), 10 μg of bovine serum albumin, 1 mM adenosine triphosphate (ATP), and 1X NEB Buffer 4 in a 100-μl

(10) HOXA‐AS2 Epigenetically Inhibits HBV Transcription by Recruiting the MTA1‐HDAC1/2 Deacetylase Complex to cccDNA MinichromosomeAdvanced ScienceJune 1, 2024YiPing Qin, JiHua Ren, HaiBo Yu, Xin He, ShengTao Cheng, WeiXian Chen, Zhen Yang, FengMing Sun, ChunDuo Wang, SiYu Yuan, et al.extraction, and ethanol precipitation. The extracted DNA was subsequently digested with exonuclease V (M0345S, New England Biolabs, USA) for 30 min at 37 °C to remove linear genomic DNA and HBV replication intermediates.

(11) ZNF148 inhibits HBV replication by downregulating RXRα transcriptionVirology JournalJanuary 31, 2024Xinyan Yao, Kexin Xu, Nana Tao, Shengtao Cheng, Huajian Chen, Dapeng Zhang, Minli Yang, Ming Tan, Haibo Yu, Peng Chen, et al.80 °C for 5 min to denature rcDNA and double-stranded DNA (dsDNA) and incubated with exonuclease V (M0345S, New England Biolabs, MA, United States) at 37 °C for 30 min. PCR with TaqMan probes was used to measure

(12) Long-read sequencing of oropharyngeal squamous cell carcinoma tumors reveal diverse patterns of high-risk Human Papillomavirus integrationFrontiers in OncologyOctober 17, 2023Marc-Andre Gauthier, Adway Kadam, Gary Leveque, Nahid Golabi, Anthony Zeitouni, Keith Richardson, Marco Mascarella, Nader Sadeghi, Sampath Kumar Loganathanepisomes in our patient tissue samples, approximately 50ng of DNA was digested using Exonuclease V (M0345S, NEB) for 1 hour at 37°C. 10ng of the digestion products and appropriate controls were then PCR-amplified

(13) Methods for the purification and detection of single nucleotide KRAS mutations on extrachromosomal circular DNA in human plasmaCancer MedicineSeptember 1, 2023Lasse Bøllehuus Hansen, Sandra Fugl Jakobsen, Egija Zole, Julie Boertmann Noer, Li Tai Fang, Sefa Alizadeh, Julia Sidenius Johansen, Marghoob Mohiyuddin, Birgitte Regenbergplasma. 2.5 Removal of linear DNA with exonuclease V Exonuclease V kit (RecBCD, New England BioLabs, #M0345S) was used to remove lDNA. Each DNA sample was mixed with 1 mM ATP, 1X NEBuffer 4, and 24U of exonuclease

(14) Whole-genome sequencing of extrachromosomal circular DNA of cerebrospinal fluid of medulloblastomaFrontiers in OncologyNovember 8, 2022Yi Zhu, Zhihui Liu, Yuduo Guo, Shenglun Li, Yanming Qu, Lin Dai, Yujia Chen, Weihai Ning, Hongwei Zhang, Lixin Maprotocol. To remove linear DNA, the DNA was digested for 5 min with exonuclease V (New England Biolabs: #M0345S) at 37°C, as recommended by the manufacturer’s protocol. The circular structure of eccDNA was opened

(15) An Exonuclease V–qPCR Assay to Analyze the State of the Human Papillomavirus 16 Genome in Cell Lines and TissuesCurrent Protocols in MicrobiologySeptember 12, 2022Julia E. Myers, Katarzyna Zwolinska, Martin J. Sapp, Rona S. ScottPromega, E1751) Nuclease-free water Exonuclease V (recBCD, 10,00 units/mL; e.g., New England Biolabs, M0345S) containing: 10 mM ATP 10× buffer Power SYBR® Green PCR Master Mix (e.g., Applied Biosystems, 4367659)

(16) Translocation of mitochondrial DNA into the nuclear genome blurs phylogeographic and conservation genetic studies in seabirdsRoyal Society Open ScienceJune 15, 2022Torres Lucas, Bretagnolle Vincent, Pante Ericin [30]. To prevent numt co-amplification, we digested nuclear DNA with the ExonucleaseV (ExoV; NEB-M0345S), using the following protocol modified from Jayaprakash et al. [36]. One microgram of gDNA was heated

(17) Restriction of viral gene expression and replication prevents immortalization of human keratinocytes by a beta-human papillomavirusProceedings of the National Academy of SciencesMarch 15, 2022Tina M. Rehm, Elke Straub, Thomas Iftner, Frank Stubenrauchmodifications: total cellular DNA (100 ng) was incubated in the presence or absence of 5 U exonuclease V (NEB M0345S) in 1× NEBuffer 4 supplemented with 1 mM ATP for 60 min at 37 °C. Then, the enzyme was inactivated for

(18) Whole Genome Assembly of Human Papillomavirus by Nanopore Long-Read SequencingFrontiers in GeneticsJanuary 4, 2022Shuaibing Yang et al.(no episomal HPV) (Yee et al., 1985), the extracted DNAs were treated with or without exonuclease V (M0345S, NEB) at 37°C for 3 h. A pair of divergent primers (primer 18 F, R) located in the L1 region of HPV35

(19) Pimobendan Inhibits HBV Transcription and Replication by Suppressing HBV Promoters ActivityFrontiers in pharmacologyJanuary 1, 2022Yuan SY, Yu HB, Yang Z, Qin YP, Ren JH, Cheng ST, Ren F, Law BYK, Wong VKW, Ng JPL, et al.80 °C for 5 min and cooling on ice immediately to denature rcDNA; 2) incubating with exonuclease V (M0345S, New England Biolabs, MA, United States) at 37°C for 30 min to digest rcDNA; 3) heating at 100 °C for

(20) Rapamycin inhibits hepatitis B virus covalently closed circular DNA transcription by enhancing the ubiquitination of HBxFrontiers in microbiologyJanuary 1, 2022Zhang Y, Li L, Cheng ST, Qin YP, He X, Li F, Wu DQ, Ren F, Yu HB, Liu J, et al.5 min and transferred on the ice immediately. Second, the samples were digested with exonuclease V (M0345S, New England Biolabs, MA, United States). Third, the cccDNA samples were heated at 100°C for 20 min.

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Exonuclease V (RecBCD)

Exonuclease V (RecBCD) from New England Biolabs

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Exonuclease V (RecBCD) from New England Biolabs

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