Q5 High-Fidelity DNA Polymerase M0491L from New England Biolabs

Product Specs
ItemQ5 High-Fidelity DNA Polymerase
CompanyNew England Biolabs
Price $484.00 Inquire
Catalog NumberM0491L
Size500 units
ApplicationsDNA Analysis
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulationNucleic Acids ResApril 21, 2026Caroline A McCormick, Michele Meseonznik, Yuchen Qiu, Oleksandra Fanari, Priyanka Goyal, Mitchell Thomas, Mina Shokoufandeh, Yifang Liu, Dylan Bloch, Cole Greenfield, et al.Table S1). Using the manufacturer’s protocol, the PCR reaction was set up with Q5 polymerase (NEB, M0491L). Thermocycling conditions were as follows: initial denaturation at 98°C for 30 s; 25 cycles of 98°C

(2) Disrupting pegRNA intramolecular complementarity via PBS and spacer sequence alterations can enhance prime editing efficiencyNucleic Acids ResApril 21, 2026Zsuzsanna Biczók, Sarah L Krausz, Dorottya A Simon, Eszter Tóth, Éva Varga, Tamás Annus, Flóra Huba, Máté Varga, Éva Bakos, Elfrieda Fodor, et al.NA0160-1KT) used in plasmid purifications were acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase (#M0491L), NEB Stable Competent E. coli (# C3040H) were from New England Biolabs Inc. NucleoSpin Gel and PCR Clean-up

(3) Aerobactin is a key driver of hypervirulent Klebsiella pneumoniae translocation and virulencePLoS PathogApril 13, 2026Giovanna E. Hernandez, Juan D. Valencia Bacca, Emma F. Bennett, Md. Maidul Islam, Suhrid Maiti, Noah A. Nutter, Taylor M. Young, David L. Caudell, Alicia Costa-Terryll, Jaden J. Skelly, et al.(FRT) was amplified from pKD4 with 50 bp homology to iucA at the 5’ and 3’ ends, using Q5 polymerase (M0491L; New England Biolabs), and treated with DpnI (1 µL/ 100 µL PCR product) to digest the pKD4 template.

(4) LINE-1 Locus Transcription Nucleates Oncogenic Chromatin ArchitectureCancer DiscoveryApril 1, 2026Michael Lee, Yuannyu Zhang, Jun Yi Stanley Lim, Tao Dai, James Ye, Margaret B. Cervantes, Varun Sondhi, Sisi Zheng, Yoon Jung Kim, Brandon Chen, et al.(Addgene, #50661). Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors

(5) Rapid CRISPR-Cas9 Genome Editing in S. cerevisiaebioRxivMarch 30, 2026Hosein Rostamian, Ethan W. Madden, Frank M. Kaplan, Riley Kim, Daniel G. Isom, Brian D. StrahlDNA),Invitrogen,15632011 Sterile water / deionized water,Corning,46-000-CI "dNTP mix (2 mM each, as used)",New England Biolabs,M0491L 5× Q5 reaction buffer,New England Biolabs,M0491L High-Fidelity DNA Polymerase,New England Biolabs,M0491L DpnI,New England Biolabs

(6) Mechanism of ribosome stalling by the AMD1 C-terminal tail arrest peptideSci AdvMarch 27, 2026Emir Maldosevic, Fabio S. Boiocchi, Michal I. Swirski, Kyle A. Meiklejohn, Martina M. Yordanova, Pavel V. Baranov, Ahmad Jomaaamplifications were carried out with Q5 High-Fidelity DNA Polymerase (New England Biolabs, catalog no. M0491L). For IVA cloning, Dpn I (New England Biolabs, catalog no. R0176L) digestion was performed to remove

(7) Biophysical trade-offs in antibody evolution are resolved by conformation-mediated epistasisbioRxivMarch 16, 2026Cole R. Tharp, Claudio Catalano, Anthony Khalifeh, Sam Ghaffari-Kashani, Ruimin Huang, Gyunghoon Kang, Giovanna Scapin, Angela M. Phillipsoverlaps to ensure correct assembly and were generated by PCR using Q5 polymerase (New England Biolabs #M0491L; primers for generating fragments are listed in SI File 3). Amplified fragments were purified using

(8) Lycium barbarum polysaccharides as prebiotics prevent colorectal cancer liver metastasis in non-alcoholic fatty liver disease by modulating gut microbiota-FGF21-PI3K-AKT axisFront PharmacolMarch 13, 2026Shuai Zhao, Zhenyao Tan, Jiaxin Suo, Yang Buperformed in a 25 μL reaction mixture containing Q5® High-Fidelity DNA Polymerase (NEB, Ipswich, MA, cat.# M0491L), 10 μmol/L of each primer, and 2 μL of DNA template. The thermal cycling protocol consisted of initial

(9) CXCR4-tropic HIV-1 infection in an immunocompetent monkey modelNature CommunicationsMarch 9, 2026Nicholas R. Meyerson, Vanessa L. Bauer, Will T. Fattor, Cody J. Warren, Arturo Barbachano-Guerrero, Melisa J. Weiss, Obaiah Dirasantha, Bridget L. Burris, Adetunji S. Adesina, Joseph L. Timpona, et al.Tetherin, TRIMCyp, and APOBEC3G from cDNA using Q5 High-Fidelity DNA Polymerase (New England BioLabs #M0491L). All PCR products were treated with Exonuclease I (Thermo Fisher Scientific #70073) and recombinant

(10) G-quadruplexes regulate chromatin accessibility and gene expression in Bloom SyndromebioRxivMarch 5, 2026Dingwen Su, Veronika Altmannova, Volker Soltys, Moritz Peters, Christopher M Cunniff, John R Weir, Yingguang Frank Chanthen amplified via PCR (10 cycles) using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Cat. #M0491L) and indexed primers N5xx and N7xx (synthesized by Integrated DNA Technologies) (Supplementary Table). BS

(11) Regulation of mitophagy by Fis1 and Fascin1-organized actinCurr BiolFebruary 24, 2026Shintaro Nakajima, Ting-Yu Wang, Tsui-Fen Chou, Yogaditya Chakrabarty, David C. Chanpolymerase chain reaction (PCR) from total mRNA extracted from WT MEFs using Q5 High-Fidelity DNA Polymerase (M0491L; New England Biolabs). For the non-tagged mouse Fis1 construct, amplified mouse Fis1 was cloned into

(12) Trimodal single-cell profiling of transcriptome, epigenome, and 3D genome in complex tissues with scHiCARNature BiotechnologyFebruary 19, 2026Xiaolin Wei, Yueyuan Xu, Dongchan Yang, Kyukwang Kim, Lizhi Yi, Wenzhe Luo, Xin Lin, Yu Xiang, Ashley B. Williams, Xiaotao Wang, et al.of 10 mM dNTP, 1.6 μl of P5 (10mM), 1.6 μl of Truseq-pcr-i7 (10 mM), 0.8 μl of Q5 polymerase (NEB, m0491L) and 28.4 μl of ultrapure water) was added to the purified DNA. PCR was performed according to the following

(13) Molecular basis for anti-jumbo phage immunity by AVAST type 5Molecular CellFebruary 19, 2026Aswin Muralidharan, Ana Rita Costa, Desi Fierlier, Daan Frits van den Berg, Halewijn van den Bossche, Adja Damba Zoumaro-Djayoon, Alicia Rodríguez-Molina, Martin Pabst, Martin Pacesa, Bruno E. Correia, et al.Precision Plus Protein- Dual Xtra Prestained Protein Standards,Bio-Rad,Cat# 1610377 Q5 DNA polymerase,New England Biolabs,Cat# M0491L Q5 High GC Enhancer,New England Biolabs,Cat# M4093 Rhamnose,Fisher Scientific,Cat# 10583731 Sequencing Grade Modified Trypsin

(14) Systematic Identification of Germ Granule Proteins Reveals Specialized Roles in RNAi and Small RNA InheritancebioRxivFebruary 1, 2026Shihui Chen, Lucas H. Prescott, Craig C. Mello, Carolyn M. Phillips(Thermo Fisher 18080–051). Small RNA libraries were amplified using Q5 High-Fidelity DNA polymerase (NEB M0491L) and size selected on a homemade 10% polyacrylamide gel. Library concentration was determined using

(15) Ubiquitous low-energy RNA fluctuations and energetic coupling measured by chemical probingbioRxivFebruary 1, 2026Edric K. Choi, Ritwika Bose, David H. Mathews, Anthony M. Mustoe, Julius B. Luckstemplates were ordered as custom gBlocks (IDT) and amplified by PCR using Q5 DNA Polymerase (NEB Cat. No. M0491L) and relevant primers (table S2). Large-scale in vitro transcription reactions (1–3 mL) were performed

(16) Tunable Chemical and Optical Control of ER-Plasma Membrane Contact Site Geometry and Dynamics with High-Fidelity VisualizationbioRxivJanuary 31, 2026Shaoqing Zhang, Jinyu Fei, Yuanmin Zheng, Ruobo Zhouproviders. Coding sequences were amplified by PCR using high-fidelity DNA polymerase (New England Biolabs, M0491L), and plasmids were assembled using Gibson assembly (New England Biolabs, E5510S) according to the manufacturer’s

(17) Workflow for Gene Overexpression and Phenotypic Characterisation in Taraxacum kok-saghyzMethods and ProtocolsJanuary 24, 2026Loredana Lopez, Michele Antonio Savoia, Loretta Daddiego, Paolo Facella, Elio Fantini, Linda Bianco, Simone Maci, Francesco PanaraCat. no.: 6784-2504).Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA; Cat. no.: M0491L).QIAwave Plasmid Miniprep Kit (Qiagen, the Netherlands; Cat. no.: 27204).Rifampicin (Merck, Darmstadt,

(18) Monitoring Gene Expression in Retina with synthetic serum markersbioRxivJanuary 21, 2026Jiaxiong Lu, Sangsin Lee, Meng Wang, Karen Zheng, Soo Hwan Oh, Jocelyn Lee, Sujung Soh, Yexuan Cao, Xuan Bao, Yifan Huang, et al.pAAV-hSyn-GlucRMA-IRES-EGFP (Addgene #189629). The PCR was conducted using Q5® High-Fidelity DNA Polymerase (NEB #M0491L). Finally, Gibson Assembly was employed with NEBuilder® HiFi DNA Assembly Master Mix (NEB #E2621L) to

(19) Multiplexed lipid nanoparticle barcoding reveals tissue-dynamic kinetic insights and enriched cellular tropism in hepatic zonesNature CommunicationsJanuary 7, 2026Stephen T. Moore, Xizhen Lian, Amogh Vaidya, Sumanta Chatterjee, Julien Santelli, Yehui Sun, Lukas Farbiak, Hao Zhu, Daniel J. Siegwartperformed as described above. Full-length Cre amplicons were obtained via PCR with Q5 DNA polymerase (NEB, M0491L) using primers that anneal the 5’ UTR and 3’ UTR, followed by agarose gel electrophoresis and purification

(20) Bacterial population dynamics during colonization of solid tumorsMolecular Systems BiologyDecember 15, 2025Serkan Sayin, Motasem ElGamel, Brittany Rosener, Michael Brehm, Andrew Mugler, Amir Mitchell12605010 Phosphate-buffered saline (PBS),Corning,Cat# 21-040-CV Q5 DNA polymerase,New England Biolabs,Cat# M0491L Agarose,Fisher Scientific,Cat# BP160500 10X TRIS-ACETATE-EDTA TAE Buffer,Fisher Scientific,Cat# BP13354 Disposable

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Q5 High-Fidelity DNA Polymerase

Q5 High-Fidelity DNA Polymerase from New England Biolabs

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Q5 High-Fidelity DNA Polymerase from New England Biolabs

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