SpeI-HF R3133L from New England Biolabs

Product Specs
ItemSpeI-HF
CompanyNew England Biolabs
Price $308.00 Inquire
Catalog NumberR3133L
Size2500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) AKT-mediated phosphorylation of TSC2 controls stimulus- and tissue-specific mTORC1 signaling and organ growthDevelopmental CellOctober 6, 2025Yann Cormerais, Samuel C. Lapp, Krystle C. Kalafut, Madi Y. Cissé, Jong Shin, Benjamin Stefadu, Jean Personnaz, Sandra Schrötter, Jessica Freed, Angelica D’Amore, et al.atcatgtccagacaggtttcc-3’); the PCR product was then purified (Qiagen, 28106), digested with SpeI enzyme (NEB, R3133L), and resolved on an agarose gel. Location of the primers and representative PCR results are displayed

(2) Effects of Aging on Z-DNA-Induced Genetic Instability In VivoGenes (Basel)August 11, 2025Tonia T. Li, Alexandra D’Amico, Laura Christensen, Karen M. Vasquezfrom the mouse tissues and digested with SpeI-HF enzyme (New England Biolabs, Ipswich, MA, USA, REF: R3133L). The genomic DNA was then purified via phenol–chloroform extraction and ethanol precipitation before

(3) Adeno-associated virus vector modification based on directed evolution technology for gene therapy targeting head and neck squamous cell carcinomaFront OncolJune 3, 2025Yiyuan Zhu, Wei Ji, Qi Zhang, Yanbo Dong, Liangfa LiuPCR product was gel extracted and digested overnight with SpeI-HF and NotI-HF (both from New England Biolabs, R3133L and R3189L, respectively). The digested fragment was ligated into a similarly digested, replication

(4) Shigella flexneri Outbreak at a Rehabilitation Center: First Report from Saudi ArabiaHealthcare (Basel)April 23, 2025Khalifa Binkhamis, Sarah Alangari, Fatema Juma, Sahar Althawadi, Ahmed A. Al-Qahtani, Marie Fe F. Bohol, Fatimah S. Alshahrani, Fawzia Alotaibi(New England Biolabs, Ipswich, MA, USA, Catalog No. R0189L) and/or SpeI (New England Biolabs, MA, USA, Catalog No. R3133L). The plugs were then washed once for 30 min at 37 °C with 0.5 mL of 0.5X Tris-Boric acid-EDTA (TBE)

(5) A highly stable lyophilized mRNA vaccine for Herpes Zoster provides potent cellular and humoral responsesNPJ VaccinesMarch 14, 2025Raquel Munoz-Moreno, Viola Allaj, Eddie Gadee, Julie M. Button, Fernando Diaz, Mohan S. Maddur, Wei Chen, Cheng Hui Hu, Lyndsey Martinez, Andreas Giannakou, et al.assembly (NEB Builder HiFi DNA Assembly NC0825276) or standard restriction digestion with SpeI (NEB, R3133L) and XhoI (R0146L) followed by ligation (Promega, PR-M1794) into our proprietary linearized vector were

(6) Loop-extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complexMolecular CellJanuary 2, 2025Biswajit Pradhan, Amar Deep, Jessica König, Martin Dieter Baaske, Kevin D. Corbett, Eugene Kimbiotin handle and cosmid-I95 DNA were both digested for 2 h at 37°C with SpeI-HF (New England Biolabs, R3133L) and subsequently heat-inactivated for 20 min at 80°C, resulting in linear ~44-kbp DNA and ~600-bp biotin

(7) Repair of replication-dependent double-strand breaks differs between the leading and lagging strandsMolecular CellJanuary 2, 2025Michael T. Kimble, Aakanksha Sane, Robert J.D. Reid, Matthew J. Johnson, Rodney Rothstein, Lorraine S. Symington,New England Biolabs,Cat# R0104S ClaI,New England Biolabs,Cat# R0197S SpeI-HF,New England Biolabs,Cat# R3133L "dATP, dTTP, dCTP, dGTP",ThermoFisher,Cat# R0181 Alpha factor,Genscript,Cat# RP01002 Pronase,Millipore,Cat#

(8) Protocol for deriving human preimplantation epiblast stem cells and 8-cell embryo-like cellsSTAR ProtocolsDecember 20, 2024Shiqi Liang, Xiu Yu, Changlei Zhou, Jichang Wang, Mingzhu Yang, Chuanbo Sunprophylactic,InvivoGen,Ant-mpp Primocin,InvivoGen,ant-pm-2 Horse serum,Biosharp,BL209A Triton X-100,Sigma,Cat#X100-100 mL SpeI-HF,NEB,R3133L MluI-HF,NEB,R3198L ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, EZ-press

(9) Identification of suitable target/E3 ligase pairs for PROTAC development using a rapamycin-induced proximity assay (RiPA)eLifeDecember 6, 2024Bikash Adhikari, Katharina Schneider, Mathias Diebold, Christoph Sotriffer, Elmar Wolfprotein",MluI-HF,New England BioLabs,Cat# R3198L, "Peptide, recombinant protein",SpeI-HF,New England BioLabs,Cat# R3133L, "Peptide, recombinant protein",XhoI,New England BioLabs,Cat# R0146L, Commercial assay or kit,Nano-Glo Luciferase Assay

(10) Downregulation of miRNA-26a by HIV-1 Enhances CD59 Expression and Packaging, Impacting Virus Susceptibility to Antibody-Dependent Complement-Mediated LysisVirusesJuly 4, 2024Nicolas Bellini, Chengyu Ye, Oluwaseun Ajibola, Thomas T. Murooka, Robert Lodge, Éric A. CohenVif-deficient (ΔVif) isogenic derivative was generated by inserting an SpeI (NEB, Ipswich, MA, USA, #R3133L) to SalI (NEB, #R3138L) fragment encompassing a vif deletion from the NL–PIvif-construct [34] into NL4.3-ADA-GFP. The

(11) Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegansDevelopmental CellJuly 1, 2024Cecilia G. Martin, James S. Bent, Tyler Hill, Irini Topalidou, Aakanksha Singhvi,New England Biolabs,Cat#R3552S EcoRI-HF,New England Biolabs,Cat#R3101L SphI,New England Biolabs,Cat#R3182L SpeI,New England Biolabs,Cat#R3133L BamHI,New England Biolabs,Cat#R3136L PstI,New England Biolabs,Cat#R0140L NotI,New England Biolabs,Cat#R0189L XbaI,New England Biolabs

(12) Mechanisms underlying Myosin 10′s contribution to the maintenance of mitotic spindle bipolarityMolecular Biology of the CellFebruary 1, 2024Yang-In Yim, Antonio Pedrosa, Xufeng Wu, Krishna Chinthalapudi, Richard E. Cheney, John A. Hammer96930). The Xlone-GFP backbone plasmid was linearized by treating with restriction enzymes SpeI-HF (NEB R3133L) and KpnI-HF (NEB R3142L). To create the rescued cell lines, KO-1 cells were nucleofected using an Amaxa

(13) Supercoiling-dependent DNA binding: quantitative modeling and applications to bulk and single-molecule experimentsNucleic Acids ResearchJanuary 11, 2024Pauline J Kolbeck, Miloš Tišma, Brian T Analikwu, Willem Vanderlinden, Cees Dekker, Jan Lipfertand we digested both the biotin handle and large 42 kb DNA plasmid with SpeI-HF (New England Biolabs, R3133L) for 2 h at 37 °C. The reaction was stopped by subsequent heat-inactivation for 20 min at 80 °C. This

(14) Recombinant cellular model system for human muscle-type nicotinic acetylcholine receptor α1(2)β1δεCell stress & chaperonesNovember 1, 2023Brockmöller S, Seeger T, Worek F, Rothmiller S.performed with Spin Miniprep Kit by QIAGEN. Plasmids were digested with AgeI-HF (R355L) and SpeI-HF (R3133L) from New England Biolabs. Inserts were purified with ReliaPrep DNA clean-up and concentration system

(15) Transcription activator-like effector protects bacterial endosymbionts from entrapment within fungal hyphaeCurrent BiologyJuly 10, 2023Ingrid Richter, Philipp Wein, Zerrin Uzum, Claire E. Stanley, Jana Krabbe, Evelyn M. Molloy, Nadine Moebius, Iuliia Ferling, Falk Hillmann, Christian HertweckHigh-Fidelity PCR Master Mx,New England Biolabs,CAT#M0530L Apramycin,Sigma,CAT#A2024 SpeI,New England Biolabs,CAT#R3133L KpnI,New England Biolabs,CAT#R3142L OneTAQ® Quick-Load® 2x Master Mix,New England Biolabs,CAT#M0486L Ethidium

(16) Myosin 10 uses its MyTH4 and FERM domains differentially to support two aspects of spindle pole biology required for mitotic spindle bipolaritybioRxivJune 20, 2023Yang-In Yim, Antonio Pedrosa, Xufeng Wu, Krishna Chinthalapudi, Richard E. Cheney, John A. Hammer96930). The Xlone-GFP backbone plasmid was linearized by treating with restriction enzymes SpeI-HF (NEB R3133L) and KpnI-HF (NEB R3142L). To create the rescued cell lines, HeLa Myo10 KO1 cells were nucleofected

(17) Identification of an integrase-independent pathway of retrotranspositionScience AdvancesJuly 1, 2022Feng Li, Michael Lee, Caroline Esnault, Katie Wendover, Yabin Guo, Paul Atkins, Mikel Zaratiegui, Henry L. LevinThermomixer for 16 hours at 16°C, followed by 15 min at 65°C. Spe I/BspH I digestion Spe I (8 μl; NEB, R3133L) or BspH I (NEB, R0517L), 24 μl of 10× NEB Cutsmart buffer (NEB, B7204S), and 208 μl of ddH2O were added

(18) Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinctionScientific reportsMarch 8, 2022Zywitza V, Rusha E, Shaposhnikov D, Ruiz-Orera J, Telugu N, Rishko V, Hayashi M, Michel G, Wittler L, Stejskal J, et al.instructions. 10 µg of NWR genomic DNA and coMIP247 control DNA were digested with SPE1-HF (NEB, # R3133L) at 37 °C overnight. Digested DNA was subjected to gel electrophoresis at 55 V for 7 h on a 0.8% LE

(19) Generation of auxin inducible degron (AID) knock-in cell lines for targeted protein degradation in mammalian cellsSTAR ProtocolsDecember 17, 2021Bikash Adhikari, Ashwin Narain, Elmar WolfGmbH,Cat#FBS-11A Penicillin-Streptomycin,Sigma-Aldrich,Cat#P4333 Blasticidin,InvivoGen,Cat#ant-bl SpeI-HF,New England BioLabs,Cat#R3133L MluI-HF,New England BioLabs,Cat#R3198L AgeI-HF,New England BioLabs,Cat#R3552L BbsI-HF,New England BioLabs

(20) Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombinationMolecular CellOctober 1, 2021Jasvinder S. Ahuja, Catherine S. Harvey, David L. Wheeler, Michael LichtenAldrich,11119915001 Zymolyase,MP Biomedicals,08320932 XmnI,New England Biolabs,R0194L SpeI-HF,New England Biolabs,R3133L XcmI,New England Biolabs,R0533L HaeIII,New England Biolabs,R0108L NdeI,New England Biolabs,R0111S DdeI,New England Biolabs

Be the First to Write a Review!

SpeI-HF

SpeI-HF from New England Biolabs

Description

New England Biolabs

Citations (28)

Reviews

SpeI-HF from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map