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Hot StartTaq2X Master Mix
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Hot StartTaq2X Master Mix
Hot StartTaq2X Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(21)
$65.00 Inquire
from
New England Biolabs for
Hot StartTaq2X Master Mix
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Hot StartTaq2X Master Mix
Company
New England Biolabs
Price
$65.00 Inquire
Catalog Number
M0496S
Size
100 reactions
Applications
DNA Analysis
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (21)
(1)
Muscleblind-like proteins dimerize by forming disulfide bonds to regulate alternative splicing and pathogenic RNA foci formation
bioRxiv
March 30, 2026
Luke A. Knudson, Adam Kosti, Kathryn R. Moss, Liang Shi, GiaLinh N. Nguyen, Aleksandra Janusz-Kaminska, Eric X. Zhou, Ryan P. Hildebrandt, Eric T. Wang, Gary J. Bassell
except RTB300 which was amplified 28 cycles, using Hot Start
Taq 2x Master Mix
(
New England Biolabs
,
M0496S
). The Wnk1 products were run on a 1% TBE agarose gel with SYBR Safe (
Invitrogen
,
S33102
), the Tcea2
(2)
RNA innate immunity constitutes a barrier for interspecies chimerism
Cell
January 8, 2026
Yingying Hu, Hai-Xi Sun, Masahiro Sakurai, Zhou Luo, Amanda E. Jones, Tianlei Cheng, Jia Huang, Lizhong Liu, Canbin Zheng, Jie Li,
et al.
protein,Takara Bio,#632678 Cy5-labelled GFP mRNA,OZ Biosciences,#MRNA11C Hot Start
Taq 2x Master Mix
,
NEB
,#
M0496L
NEBuilder® HiFi DNA Assembly Master Mix
,
NEB
,#
E2621L
SYBR Green Master Mix,
Qiagen
,#
330502
iScript™
(3)
Defects in DNA damage signaling and cell cycle checkpoints in a mouse model of Rhno1 deletion
Cell Death Discovery
December 19, 2025
Joonyoung Her, Adithi Santhosh, Yanira Gonzalez-Rodriguez, Niphat Jirapongwattana, Channabasavaiah B. Gurumurthy, Adam R. Karpf, Samuel F. Bunting
equivalent
RT-PCR
products produced using the primers as listed above with Hot Start
Taq 2x Master Mix
(
M0496
;
NEB
) under unsaturated conditions. Class switch recombination junction analysis For
cloning
of repair
(4)
Multiplexed ultrasound imaging of gene expression
Nature Methods
November 18, 2025
Nivin N. Nyström, Zhiyang Jin, Marisa E. Bennett, Ruby Zhang, Margaret B. Swift, Mikhail G. Shapiro
library construction
and expression All
PCR reactions
were performed using the Taq Hot Start System (cat. no.
M0496
,
NEB
) and fragment insertion was conducted with the High Fidelity DNA Assembly System (cat. no.
M0541
,
(5)
TYROSINASE-Deficient Human Retinal Pigment Epithelium Exhibits Melanosome Maturation Defects
Invest Ophthalmol Vis Sci
October 1, 2025
Aman George, Tyler Pfister, Charles DeYoung, Ruchi Sharma, Mones Abu-Asab, Jizhong Zou, Kapil Bharti, Brian P. Brooks
primers 5’- GTTTGATGCTGGAGGTGGGA -3’ and 5’-TGGTCCCCAAAAGCCAAACT-3’ with Hot Start
Taq 2X Master Mix
(
M0496
;
New England Biolabs
, Ipswich, MA, USA) and Sanger sequenced (Eurofins Genomics, Ebersberg, Germany)
(6)
TBK1 is involved in programmed cell death and ALS-related pathways in novel zebrafish models
Cell Death Discov
March 12, 2025
Quentin Raas, Gregoire Haouy, Hortense de Calbiac, Elena Pasho, Anca Marian, Ida Chiara Guerrera, Marion Rosello, Patrick Oeckl, Filippo Del Bene, Alberto Catanese,
et al.
3’-GCTCGTCATGCTAACTCCACT-5’ For each sample, 2 μL of DNA was mixed with 10 μL of Hot Start
Taq 2X Master Mix
(
NEB
,
M0496
), 0.8 μL of each primer and 6.4 μL of sterile water. The
PCR amplification
program was optimized for
(7)
A high-throughput protein tagging toolkit that retains endogenous untranslated regions for studying gene regulation in kinetoplastids
Open Biol
February 26, 2025
Carla Gilabert Carbajo, Xiaoyang Han, Bhairavi Savur, Arushi Upadhyaya, Fatima Taha, Michele Tinti, Richard J. Wheeler, Phillip A. Yates, Calvin Tiengwe
edited loci were performed in a 25 μl reaction volume using Hot Start
Taq 2X Master Mix
(
NEB
, cat. no.
M0496
). Five microlitres of all
PCR reactions
were resolved on a 1% agarose gel to verify the presence of
(8)
Optimizing Yeast Homologous Recombination for Splicing Large Coronavirus Genome Fragments
International Journal of Molecular Sciences
December 23, 2024
Guoqing Xiong, Xuan Huang, Ao Hu, Zhixin Meng, Jiazhen Cui, Yuzhong Feng, Zhili Chen, Yuanyuan Lu, Qi Yang, Gang Liu
μM) and 1.0 µL of downstream primer (10 μM), along with 25.0 µL of Hot Start Taq 2× Master Mix (
NEB
,
M0496S
, Ipswich, USA), 22.5 µL of nuclease-free water, and 0.5 µL of
yeast
cell lysis
dilution. 4.5. Evaluation
(9)
Human stem cell-specific epigenetic signatures control transgene expression
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
December 1, 2024
Chulhwan S. Kwak, Furkan E. Oflaz, Jiamin Qiu, Xinnan Wang
primers (sequences provided in the Table below), and 2xPCR master mix (
M0492S
, Q5 Polymerase,
NEB
or
M0496L
, HotStart
Taq Polymerase
, NEB) was incubated using the following thermocycling steps: 95 °C for 30 s,;
(10)
Automating life science labs at the single-cell level through precise ultrasonic liquid sample ejection: PULSE
Microsystems & Nanoengineering
November 20, 2024
Peiran Zhang, Zhenhua Tian, Ke Jin, Kaichun Yang, Wesley Collyer, Joseph Rufo, Neil Upreti, Xianjun Dong, Luke P. Lee, Tony Jun Huang
format on a substrate and imaged under a microscope. Then, 400 nL of 0.89 ×
Taq-polymerase
master mix (
M0496
,
NEB
, USA) was added to each spot of the cell-loaded substrate. Note that the concentration of the master
(11)
Methanotrophic Communities and Cultivation of Methanotrophs from Rice Paddy Fields Fertilized with Pig-livestock Biogas Digestive Effluent and Synthetic Fertilizer in the Vietnamese Mekong Delta
Microbes and Environments
October 2, 2024
Huynh Van Thao, Mitsunori Tarao, Hideshige Takada, Tomoyasu Nishizawa, Tran Sy Nam, Nguyen Van Cong, Do Thi Xuan
first
PCR product
was prepared in a 25-μL mixture as follows: 12.5 mL Hot Start Taq 2× Master Mix (
M0496L
;
New England Biolabs
); 0.5 μL each of 10 μmol L–1 A189f and mb661r primers; 5 μL of template DNA
(12)
Genome Sequence of Fowlpox Virus-Integrated Reticuloendotheliosis Virus from a Rio Grande Wild Turkey (Meleagris gallopavo
intermedia
)
Microbiology Resource Announcements
June 16, 2022
Bianca Willis, Camille Trautman, Faith Cox, Tiffany Lujan, Jason Hardin, Robert Dittmar, Camila Romano, Jeff Brady, Dustin Edwards
isolate TSU-1029 contained an REV proviral genome insertion,
PCR
using Hot Start Taq 2× master mix (
NEB
;
M0496L
) and the heterologous primers REV env 7F and FPV 203 4R produced an appropriately sized 740-bp product
(13)
NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes
Nucleic acids research
May 20, 2022
Bao H, Carraro M, Flury V, Liu Y, Luo M, Chen L, Groth A, Huang H.
10 minutes. 2 μl of this lysate was directly used for
PCR reactions
with 2 × Taq start master mix (
M0496L
,
NEB
), and the two
NASP
clone
screening
primers (see primer table). Amplified DNA was sequenced using
(14)
Reconstructing the Lineage Histories and Differentiation Trajectories of Individual Cancer Cells in Myeloproliferative Neoplasms
Cell stem cell
March 4, 2021
Van Egeren D, Escabi J, Nguyen M, Liu S, Reilly CR, Patel S, Kamaz B, Kalyva M, DeAngelo DJ, Galinsky I,
et al
.
previously. In Step 3, a 25uL
PCR
mixture was made containing 12.5uL of Hot Start
Taq 2x Master Mix
(
NEB
#
M0496S
), 1.25uL of cDNA additive (10x Genomics #220067), 1.25uL of forward primer (Partial P5, AATGATACGGCGACCACCGAGATCT)
(15)
Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP)
FASEB BioAdvances
March 1, 2020
Julian Rubinfien et al.
Q5 Hot Start High‐Fidelity 2X Master Mix
(
NEB
M0494
; New England Biolabs, Inc) or Hot Start
Taq 2X Master Mix
(
NEB
M0496
; New England Biolabs, Inc), along with 5 μL primers prepared at 5 μmol/L concentration, 18 μL distilled
(16)
Recovery of Renal Function following Kidney-Specific VEGF Therapy in Experimental Renovascular Disease
American journal of nephrology
January 1, 2020
Engel JE, Williams ML, Williams E, Azar C, Taylor EB, Bidwell GL, Chade AR.
(
Thermo Scientific
,
K1621
).
qPCR
was performed using Hot Start
Taq 2x Master Mix
(
New England BioLabs
,
M0496L
). Samples were probed using primer pairs for Beta Actin (Hs01060665_g1), VEGF (Hs00900055_m1), KDR/Flk-1
(17)
Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS
Cell
December 12, 2019
Chao CC, Gutiérrez-Vázquez C, Rothhammer V, Mayo L, Wheeler MA, Tjon EC, Zandee SEJ, Blain M, de Lima KA, Takenaka MC,
et al
.
DAPI Fluoromount-G
,
SouthernBiotech
,Cat#
0100–20
NuPAGE LDS sample buffer,
Invitrogen
,Cat#
NP0007
Hot Start
Taq 2X Master Mix
,
New England Biolabs
,Cat#
M0496
FuGENE HD Transfection Reagent
,
Promega
,Cat#
E2311
UK-5099
,
Tocris Bioscience
,Cat#4186 methylglyoxal
(18)
Demonstration of End-to-End Automation of DNA Data Storage
Scientific Reports
December 1, 2019
Christopher N. Takahashi, Bichlien H. Nguyen, Karin Strauss, Luis Ceze
kilobase fragment that was
PCR
-amplified from the lambda genome using hot start TAQ DNA polymerase (
NEB
M0496
) with a Bsa-I restriction site added by the forward primer. The resulting fragment after digestion had
(19)
Aptamers in the Therapeutics and Diagnostics Pipelines
Theranostics
July 1, 2018
Harleen Kaur, John G. Bruno, Amit Kumar, Tarun Kumar Sharma
enzymes and other molecular biology reagents, has commercialized Hot Start Taq DNA polymerase (Cat No.
M0496S
and
M0490S
). Both of these products principally rely on an aptamer-based hot start technology to avoid
(20)
Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer
Journal of Bacteriology
April 1, 2017
Anthony W. Kingston, Christine Ponkratz, Elisabeth A. Raleigh
(catalog number
M0491
;
NEB
), while diagnostic PCRs used the Hot Start Taq 2× master mix (catalog number
M0496
;
NEB
). Matings. Donor and recipient cultures were grown at 37°C to an optical density at 600 nm (OD600)
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Hot StartTaq2X Master Mix from New England Biolabs
Price
$65.00
Inquire
Product Specs
Company Info
Citations
Reviews
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