Bacteroides Heparinase III P0737L from New England Biolabs

Product Specs
ItemBacteroides Heparinase III
CompanyNew England Biolabs
Price $694.00 Inquire
Catalog NumberP0737L
Size35 units
ApplicationsProteomics & Glycomics
Temperature-80 C
TypeGlycosidases

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

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Website: http://www.neb.com

(1) Extracellular Vesicles Coordinate Bacterial Cloaking in Lung Epithelial Cells to Alleviate Acute Inflammatory InjuryJ Extracell VesiclesFebruary 8, 2026Feng Ding, Shengkai Gong, Haotian Luo, Dandan Wu, Xiaoshan Yang, Zihan Li, Dingmei Zhang, Peijie He, Jiani Liu, Lili Bao, et al.min), Amiloride (MedChemExpress, HY‐B0285; 1 mmol/L, 30 min), Heparinase III (New England Biolabs, P0737S; 10 mU, 30 min), Rho Inhibitor I (Cytoskeleton, CT04; 0.5 µg/mL, 4 h), SPA (Sino Biological, 10600‐P07E;

(2) Novel regulators of heparan sulfate proteoglycans modulate cellular uptake of α-synuclein fibrilsCommun BiolOctober 6, 2025Benoît Vanderperre, Amitha Muraleedharan, Marie-France Dorion, Frédérique Larroquette, Esther Del Cid Pellitero, Nishani Rajakulendran, Carol X.-Q. Chen, Roxanne Larivière, Charlotte Michaud-Tardif, Thomas Goiran, et al.with a cocktail of heparinase I (NEB, P0735S), heparinase II (NEB, P0736S) and heparinase III (NEB, P0737S) at 3 U/mL each. GFP+ binding assay was performed as for PFFs binding assay, except cells were treated

(3) Nanoscopy Reveals Heparan Sulfate Clusters as Docking Sites for SARS-CoV-2 Attachment and EntrybioRxivSeptember 16, 2025Sue Han, Xin Wang, Tiansheng Li, Ammar Mohseni, Ivan Kosik, Chung Yu Chan, Alberto Domingo López-Muñoz, Jessica Matthias, Reid Suddaby, Zhixiong Wang, et al.(3.2 units, NEB, #P0735S), heparinase II (1 units, NEB, #P0736S) and heparinase III (0.19 units, NEB, #P0737S), was applied to cells in FBS-free DMEM at 30°C for 1 h before the virus was applied. After washing

(4) Recombinant Small Leucine-Rich Proteoglycans Modulate Fiber Structure and Mechanical Properties of Collagen GelsACS Biomaterials Science & EngineeringJuly 14, 2025Serafina G. Lopez, Henrique Reis Moura, Erik Chow, Joe Chin-Hun Kuo, Matthew J. Paszek, Lawrence J. BonassarμL of each purified sample, Chondroitinase ABC (Sigma, C3667), Heparinase III (New England Biolabs, P0737S), or both, were added to the samples. Chondroitinase ABC was reconstituted in a Tris buffer (50 mM Tris,

(5) Trauma promotes heparan sulfate modifications and cleavage that disrupt homeostatic gene expression in microvascular endothelial cellsFrontiers in Cell and Developmental BiologyJuly 24, 2024Robert P. Richter, James D. Odum, Camilla Margaroli, Jessica C. Cardenas, Lei Zheng, Kaushlendra Tripathi, Zhangjie Wang, Katelyn Arnold, Ralph D. Sanderson, Jian Liu, et al.conditioning, HLMVECs were exposed to vehicle (1× phosphate buffered saline) or heparinase III (500 mU/mL) (P0737L, New England BioLabs, Ipswich, MA, United States) for 6 h while 15 dyn/cm2 shear stress was maintained.

(6) Building a translational cancer dependency map for The Cancer Genome AtlasNature CancerJuly 15, 2024Xu Shi, Christos Gekas, Daniel Verduzco, Sakina Petiwala, Cynthia Jeffries, Charles Lu, Erin Murphy, Tifani Anton, Andy H. Vo, Zhiguang Xiao, et al.H1890-10) (Extended Data Fig. 8). Bacteroides heparinase III was obtained from New England Biolabs (P0737L) and used as per manufacturer’s protocol by treating cells for 1 h in reaction buffer at 30 °C before

(7) The Transpeptidase Sortase A Binds Nucleic Acids and Mediates Mammalian Cell LabelingAdvanced ScienceJune 1, 2024Yingzheng Liu, Zhike Lu, Panfeng Wu, Zhaohui Liang, Zhenxing Yu, Ke Ni, Lijia Maand treated with glycosidases (Heparinase I NEB P0735S, Heparinase II NEB P0736L, Heparinase III NEB P0737S, Chondroitinase ABC Sigma C3667, PNGase F NEB P0705S, O‐Glycosidase NEB P0733S, Hyaluronidase Sigma

(8) Isolation and Compositional Analysis of GlycosaminoglycansMethods in Molecular BiologyMay 1, 2024Stephanie Archer-Hartmann, Lauren E. Pepi, Christian Heiss, Parastoo Azadienzymes can be purchased from a number of vendor sources including New England Biolabs (P0735S, P0736S, P0737S), Sigma (H2519, H6512, H8891), GrampEnz (GE-H0001, GE-H0002, GE-H0003), or IBEX (60–010, 60–018, 60–020).

(9) Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cellsCell reports. MedicineApril 16, 2024Albanese M, Chen HR, Gapp M, Muenchhoff M, Yang HH, Peterhoff D, Hoffmann K, Xiao Q, Ruhle A, Ambiel I, et al.,New England Biolabs,Cat#P0735S Heparinase II,New England Biolabs,Cat#P0736S Heparinase III,New England Biolabs,Cat#P0737S Chondroitinase ABC from Proteus vulgaris,Merck,Cat#C3667-5UN Lipopolysaccharide (LPS) from Escherichia coli

(10) Dynamic Changes in Heparan Sulfate Nanostructure in Human Pluripotent Stem Cell DifferentiationACS NanoApril 25, 2023Deena Al Al Mahbuba, Sayaka Masuko, Shiwei Wang, Deepsing Syangtan, Jeong Seuk Kang, Yuefan Song, Tay Won Shin, Ke Xia, Fuming Zhang, Robert J. Linhardt, et al.(New England Biolabs, #P0735), heparinase II (New England Biolabs, #P0736), and heparinase III (New England Biolabs, #P0737). Each enzyme was used at 4 unit/ml concentration in the cocktail. Cells were incubated for 2 −2.5 h

(11) Cell Surface Nucleocapsid Protein Expression: A Betacoronavirus Immunomodulatory StrategybioRxiv : the preprint server for biologyFebruary 27, 2023López-Muñoz AD, Santos JJS, Yewdell JW.Bacteroides heparinase I (4.8 units), II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C. Cells were washed twice, incubated with recombinant proteins, washed, and

(12) Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunityScience advancesAugust 5, 2022López-Muñoz AD, Kosik I, Holly J, Yewdell JW.Bacteroides heparinase I (4.8 U), II (1.6 U), and III (0.28 U) (New England Biolabs, nos. P0735S, P0736S, and P0737S) in DPBS for 1 hour at 30°C. Cells were washed twice, incubated with recombinant proteins, and stained

(13) Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive ImmunitybioRxiv : the preprint server for biologyDecember 13, 2021López-Muñoz AD, Kosik I, Holly J, Yewdell JW.Bacteroides heparinase I (4.8 units), II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C. Cells were washed twice, incubated with recombinant proteins, and stained

(14) Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive ImmunityResearch squareDecember 13, 2021Yewdell J, Lopez-Munoz A, Kosik I, Holly J.Bacteroides heparinase I (4.8 units), II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C. Cells were washed twice, incubated with recombinant proteins, and stained

(15) Heparan Sulfated Glypican-4 Is Released from Astrocytes by Proteolytic Shedding and GPI-Anchor Cleavage MechanismseNeuroJuly 1, 2021Huang K, Park S.treatment Samples containing Nluc-GPC4 were treated with Heparinase II (NEB P0735S) and Heparinase III (NEB P0737S) overnight at 37°C, then subject to DEAE pulldown described above, before luciferase activity was assayed.

(16) Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in ObesityCell metabolismFebruary 2, 2021Brestoff JR, Wilen CB, Moley JR, Li Y, Zou W, Malvin NP, Rowen MN, Saunders BT, Ma H, Mack MR, et al.,New England Biolabs,Cat#P0735S Heparanase II,New England Biolabs,Cat#P0736L Heparanase III,New England Biolabs,Cat#P0737L Humulin R,Lilly,Cat#0002-8215-17 L-glutamine,Corning,Cat#25-005-Cl "Lipopolysaccharide from E. coli

(17) Host and viral determinants for efficient SARS-CoV-2 infection of the human lungNature communicationsJanuary 8, 2021Chu H, Hu B, Huang X, Chai Y, Zhou D, Wang Y, Shuai H, Yang D, Hou Y, Zhang X, et al.Caco2 cells were pretreated with Heparinase I (4U/ml) (P0735L, NEB, MA, USA), Heparinase III (0.5U/ml) (P0737L, NEB), or both at 37 °C for 1 h. Viruses were inoculated into the pretreated cells at 0.2 MOI for 2

(18) A novel circulating tamiami mammarenavirus shows potential for zoonotic spilloverPLoS neglected tropical diseasesDecember 1, 2020Moreno H, Rastrojo A, Pryce R, Fedeli C, Zimmer G, Bowden TA, Gerold G, Kunz S.and heparinase III buffer (NaCl 100 mM, Tris-HCl 20mM and CaCl2 1.5mM) with 10 IU heparinase III (Catalog number P0737S. New England Biolabs, Ipswich, Massachusetts, USA) was added and incubated for 2 hours at 30˚C and 5%

(19) Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysisMolecular OmicsApril 21, 2020Manveen K. Sethi, Margaret Downs, Joseph ZaiaScientific)Hyaluronidase (H1136-1AMP, Sigma)Chondroitinase ABC (Sigma)Heparan lyases 1,2,3 (P0735L, P0736L, P0737L New England Biolabs)Trypsin (PRV5280, Promega)/ Trypsin-LysC (PRV5072, Promega)/ Glu-C (PRV1651, Promega)High

(20) On-slide tissue digestion for mass spectrometry based glycomic and proteomic profilingMethodsXSeptember 25, 2019Rekha Raghunathan, Manveen K. Sethi, Joseph ZaiaBaker)Hyaluronidase (H1136-1AMP, Sigma)Chondroitinase ABC(Sigma)Heparan lyases 1,2,3(P0735L, P0736L, P0737L New England Biolabs)PNGase F(P0705L, New England Biolabs)Trypsin (V528A, Prozyme)Kasil (C033116, PQ

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Bacteroides Heparinase III

Bacteroides Heparinase III from New England Biolabs

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Bacteroides Heparinase III from New England Biolabs

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