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Gibson Assembly Master Mix
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Gibson Assembly Master Mix
Gibson Assembly Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(>1k)
$677.00 Inquire
from
New England Biolabs for
Gibson Assembly Master Mix
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Gibson Assembly Master Mix
Company
New England Biolabs
Price
$677.00 Inquire
Catalog Number
E2611L
Size
50 reactions
Applications
DNA Manipulation
Type
Enzymes
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (1238)
(1)
Divergent Rickettsia species exhibit distinct mechanisms of actin-based motility
Journal of Cell Biology
July 6, 2026
Meghan C. Bacher, Julie E. Choe, Jiawen Jiang, Joanna M. Idrovo, Joshua T. Del Mundo, Michal Hammel, Matthew D. Welch
rickA and sca2/6 genes included homology arms that facilitated Gibson
cloning
(
New England Biolabs
,
E2611S
) into
SspI
-cut plasmids pET His6 MBP TEV LIC (RRID:Addgene_29656) or pET His6 GST TEV LIC (RRID:Addgene_29655)
(2)
Phage-mediated lysis increases growth rate of surviving bacterial cells
iScience
June 19, 2026
Emanuele Fara, Benjamin Raach, Alessio Cavallaro, Justus Fink, Divvya Ramesh, Yongzhao Guo, Victoria Orphan, Alex R. Hall, Gabriele Micali, Martin Ackermann,
et al.
assembled using the Gibson assembly method,76 with the
NEBuilder HiFi DNA Assembly Master Mix
(
NEB
#
E2611
), following the manufacturer’s instructions. Assembled constructs were transformed into chemically competent
(3)
Engineering novel AAV capsids by broadly attenuated and subsequent muscle-specific tropism in mice and NHPs
Molecular Therapy Advances
June 11, 2026
Yue Pan, Yujian Zhong, Huan Chen, Youwei Zhang, Zhiyong Dai, Junlin Chen, Keqin Tan, Xiaoqu Chen, Danlan Qiu, Longxiang Sheng,
et al.
ligated into BsrG I (
Thermo Fisher Scientific
,
FD0933
)-digested backbone vector by Gibson assembly (
NEB
,
E2611L
). Assembled products were treated with plasmid-safe DNase (Lucigen, E3101K) to remove residual linear
(4)
ErbB family receptor dimerization dynamics and dysregulation via long-term single-molecule imaging
Cell
May 28, 2026
Kaibo Ma, Xiaojie Ma, João F. Shida, Zijian Niu, Yuzhu Karlie Lin, Saptarshi Mandal, Alexandra Dobbins, Lior Golomb, Michael J. Eck, Heidi Greulich,
et al.
GeneJET Gel Extraction and DNA Cleanup Micro Kit
,
Life Technologies
,Cat#:
K0832
Gibson Assembly Master Mix
,
New England BioLabs
,Cat#:
E2611L
ECL Western Blotting Substrate,
Life Technologies
,Cat#:
32109
DNA Clean & Concentrator-5 (Capped) 200prep,ZYMO,Cat#:
(5)
A pair of DNA glucosyltransferases elevate counter-defense in bacteriophage T4
Nucleic Acids Res
May 28, 2026
Luis Ramirez-Chamorro, Frédéric Bonhomme, Anton Lukas Ipsen Wolff, Mathieu Stouf, François Lecointe, Marcel Hollenstein, Mart Krupovic, Marianne De Paepe, Yuvaraj Bhoobalan-Chitty
PCR (polymerase chain reaction
) fragments were fused together either by Overlap Extension-
PCR
or by Gibson Assembly (
NEB
#
E2611S
) [33] and cloned into the plasmid pBAD24. In case of the positional swapping between α-gt and β-gt,
(6)
Rhythmic protein condensation in the circadian regulation of glycogen metabolism
iScience
May 15, 2026
Lily A. Burton, Gopal K. Pattanayak, Michael J. Rust
M0492S
EcoRI-HF
,
New England Biolabs
,Cat#
R3101S
Gibson Assembly Master Mix
,
New England Biolabs
,Cat#
E2611S
"cOmplete, EDTA free cocktail tablets",Sigma-Aldrich,Cat# 11873580001 Glycogen from bovine liver Type
(7)
Generation of precise and accurate engineered circRNAs using enzymatic ligation
Nucleic Acids Res
May 8, 2026
Amrita Singh, Adela Dujsikova, Noah Mueller, Y Grace Chen
T7 promoter overhang. The amplified products were cloned into pcDNA3.1 using Gibson assembly (
NEB
#
E2611L
). Plasmid sequences were confirmed by whole plasmid
sequencing
(Plasmidsaurus). Unmodified DNA templates
(8)
An anaerobic pathogen rewires host metabolism to fuel oxidative growth in the inflamed gut
Cell
April 30, 2026
Luisella Spiga, Ryan T. Fansler, Yifan Wu, Alexandra Grote, Madison Langford-Butler, Asia K. Miller, Maxwell Neal, Owen F. Hale, Deepanshu Singla, M. Wade Calcutt,
et al.
BacTiter-Glo Microbial Cell Viability Assay
,
Promega
,
G8230
Foxp3 / Transcription Factor Staining Buffer Set,
Invitrogen
,
00-5523-00
Gibson Assembly Cloning Kit
,
NEB
,
E2611
Illumina DNA Prep kit,Illumina,20060060
KAPA HiFi Hotstart ReadyMix
,
Roche
,KK2601 Lysing Matrix B,MP
(9)
GerAB residues predicted to interact with water based on MD simulations mediate germinosome stability in Bacillus subtilis spores
Frontiers in Microbiology
April 29, 2026
Longjiao Chen, Silke van Buuren, George Korza, Jocelyne Vreede, Peter Setlow, Stanley Brul
inserted in plasmid vector pUC19 with the
Gibson Assembly Master Mix
kit (
New England BioLabs
,
NEB
#
E2611S
). Plasmid- based
mutagenesis
was carried out with the
QuikChange Lightning Site-Directed Mutagenesis Kit
(10)
Co-release of tick-borne encephalitis virus RNA and structural proteins via virus-like particles and extracellular vesicles
Journal of General Virology
April 29, 2026
Ivana Křížová, Anna Klimešová, Alžběta Dostálková, Jana Racková, Jiřina Kaufmanová, Radim Novotný, Matěj Danda, Ivana Matošević, Zdeněk Franta, Hana Tykalová,
et al.
Gibson Assembly was applied according to the manufacturer’s protocol (
Gibson Assembly® Master Mix
,
NEB
#
E2611
). Individual fragments have been amplified using primers listed in Table S1 and sequence-verified. TBEV
(11)
PhoP-regulated VirK acts as an accessory factor to maintain virulence in polymyxin-resistant Klebsiella pneumoniae
Nucleic Acids Res
April 25, 2026
Haibin Li, Longyang Jin, Penghe Wang, Ruobing Wang, Qi Wang, Xiukun Wang, Congran Li, Xuefu You, Hui Wang
pTOPO-P-R (Supplementary Table S1). The assembly was conducted using
Gibson Assembly Master Mix
(Cat.
E2611S
,
NEB
, Gene Company, China) according to the manufacturer’s instructions, and transformed into electrocompetent
(12)
From Sea to Therapy: Development and Analytical Control of Recombinant Human CDKL5 Production in the Marine Bacterium Pseudoalteromonas haloplanktis TAC125
Marine Drugs
April 24, 2026
Andrea Coletti, Marzia Calvanese, Flora Cozzolino, Ilaria Iacobucci, Concetta Lauro, Angelica Severino, Maria Monti, Ermenegilda Parrilli, Maria Luisa Tutino
assembled according to the one-step isothermal DNA assembly method using Gibson assembly (
NEB
, cat #
E2611
) described by Gibson et al. (2009) [50]. To validate that the assembly was successful, the expression
(13)
TraN variants mediate conjugation species specificity of IncA/C, IncH, and Acinetobacter baumannii plasmids
Journal of Bacteriology
April 23, 2026
Shan He, Sophia David, Jaie Rattle, Julia Sanchez-Garrido, Wen Wen Low, Joshua L. C. Wong, Konstantinos Beis, Gad Frankel
using the same methodology.
Mutagenesis
vectors were generated by Gibson Assembly (
New England Biolabs
,
E2611L
) using the pSEVA612S backbone and were maintained in
E. coli
CC118λpir cells.
Site-directed mutagenesis
(14)
PEX11 mediates intralumenal vesicle formation in peroxisomes
Nat Commun
April 21, 2026
Nathan E. Tharp, Chelsea An, James Hwang, Nayeli S. Shad, Zachary J. Wright, Bonnie Bartel
(downstream of the HA tag) of the trifluorescent construct via Gibson assembly (
New England Biolabs
,
E2611
). The resultant plasmids were digested with
XhoI
and religated with Quick Ligase (
New England Biolabs
,
(15)
T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signalling
The EMBO Journal
April 21, 2026
Carmen Rodilla-Ramirez, Giorgia Carai, Eleanor Fox, Amin Zehtabian, Helen Adam, Katja Dallio, Pia Lazki-Hagenbach, Helge Ewers, Xiaolei Su, Francesca Bottanelli
Serum (FBS),Corning,35-079-CV Geneticin disulfate (G418),Roth,CP11.3
Gibson Assembly Master Mix
,
NEB
,
E2611
Glycerin,Roth,3783.1 GlutaMAX,
Gibco
,
35050061
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES),Roth,9105.2
Human
(16)
Pseudomonas aeruginosa deploys competitor-specific antagonistic strategies
bioRxiv
April 20, 2026
Ziyang Chen, Ned S. Wingreen, Zemer Gitai
Allele exchange constructs were assembled by Gibson assembly using
Gibson Assembly Master Mix
(
NEB
, cat#
E2611
) and electroporated into electrocompetent
E. coli
S17. Positive S17 transformants were selected, plasmids
(17)
Dimorphic neural network architecture prioritizes sexual-related behaviors in male Caenorhabditis elegans
Elife
April 20, 2026
Xuebin Wang, Hanzhang Liu, Wenjing Yang, Jingxuan Yang, Xuehong Sun, Qiuhan Liu, Ying Zhu, Yinghao Sun, Chunxiuzi Liu, Guiyuan Shi,
et al.
constructed via Gibson assembly (Gibson et al., 2009), utilizing the
Gibson Assembly Master Mix
(
NEB
,
E2611
) according to the manufacturer’s protocol. Gibson assembly is an isothermal, single-reaction method
(18)
Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells
Cell
April 20, 2026
Ujjwal Rathore, Eli Dugan, Hunter Thornton, Vigneshwari Easwar Kumar, Rama Dajani, Ryan C. Burdick, Janet M. Young, Zachary Steinhart, Reanna Lao, Krista A. Delviks-Frankenberry,
et al.
Dynabeads,
Thermo Fisher Scientific
,Cat#
40203D
Gibson Assembly Master Mix
- 50 rxns,
New England Biolabs
,Cat#
E2611L
GlycoBlue Coprecipitant (15 mg/mL
),
Thermo Fisher Scientific
,Cat#
AM9516
Invitrogen
Recombinant Proteinase
(19)
Dimorphic neural network architecture prioritizes sexual-related behaviors in male Caenorhabditis elegans
Elife
April 20, 2026
Xuebin Wang, Hanzhang Liu, Wenjing Yang, Jingxuan Yang, Xuehong Sun, Qiuhan Liu, Ying Zhu, Yinghao Sun, Chunxiuzi Liu, Guiyuan Shi,
et al.
constructed via Gibson assembly (Gibson et al., 2009), utilizing the
Gibson Assembly Master Mix
(
NEB
,
E2611
) according to the manufacturer’s protocol. Gibson assembly is an isothermal, single-reaction method
(20)
Force-modulated structural landscape of the catch bonding F-actin crosslinker α-actinin-4
bioRxiv
April 16, 2026
Alfred C. Chin, Fatemah Mukadum, Matthew J. Reynolds, Glen M. Hocky, Gregory M. Alushin
Plasmid assembly and
site-directed mutagenesis
were performed using Gibson assembly (
New England Biolabs
,
E2611L
) or kinase-ligase-
DpnI
(KLD)
cloning
(
New England Biolabs
,
M0554S
). All plasmids were cloned and propagated
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Gibson Assembly Master Mix from New England Biolabs
Price
$677.00
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Product Specs
Company Info
Citations
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