Gibson Assembly Master Mix E2611L from New England Biolabs

Product Specs
ItemGibson Assembly Master Mix
CompanyNew England Biolabs
Price $677.00 Inquire
Catalog NumberE2611L
Size50 reactions
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Divergent Rickettsia species exhibit distinct mechanisms of actin-based motilityJournal of Cell BiologyJuly 6, 2026Meghan C. Bacher, Julie E. Choe, Jiawen Jiang, Joanna M. Idrovo, Joshua T. Del Mundo, Michal Hammel, Matthew D. WelchrickA and sca2/6 genes included homology arms that facilitated Gibson cloning (New England Biolabs, E2611S) into SspI-cut plasmids pET His6 MBP TEV LIC (RRID:Addgene_29656) or pET His6 GST TEV LIC (RRID:Addgene_29655)

(2) Phage-mediated lysis increases growth rate of surviving bacterial cellsiScienceJune 19, 2026Emanuele Fara, Benjamin Raach, Alessio Cavallaro, Justus Fink, Divvya Ramesh, Yongzhao Guo, Victoria Orphan, Alex R. Hall, Gabriele Micali, Martin Ackermann, et al.assembled using the Gibson assembly method,76 with the NEBuilder HiFi DNA Assembly Master Mix (NEB #E2611), following the manufacturer’s instructions. Assembled constructs were transformed into chemically competent

(3) Engineering novel AAV capsids by broadly attenuated and subsequent muscle-specific tropism in mice and NHPsMolecular Therapy AdvancesJune 11, 2026Yue Pan, Yujian Zhong, Huan Chen, Youwei Zhang, Zhiyong Dai, Junlin Chen, Keqin Tan, Xiaoqu Chen, Danlan Qiu, Longxiang Sheng, et al.ligated into BsrG I (Thermo Fisher Scientific, FD0933)-digested backbone vector by Gibson assembly (NEB, E2611L). Assembled products were treated with plasmid-safe DNase (Lucigen, E3101K) to remove residual linear

(4) ErbB family receptor dimerization dynamics and dysregulation via long-term single-molecule imagingCellMay 28, 2026Kaibo Ma, Xiaojie Ma, João F. Shida, Zijian Niu, Yuzhu Karlie Lin, Saptarshi Mandal, Alexandra Dobbins, Lior Golomb, Michael J. Eck, Heidi Greulich, et al.GeneJET Gel Extraction and DNA Cleanup Micro Kit,Life Technologies,Cat#: K0832 Gibson Assembly Master Mix,New England BioLabs,Cat#: E2611L ECL Western Blotting Substrate,Life Technologies,Cat#: 32109 DNA Clean & Concentrator-5 (Capped) 200prep,ZYMO,Cat#:

(5) A pair of DNA glucosyltransferases elevate counter-defense in bacteriophage T4Nucleic Acids ResMay 28, 2026Luis Ramirez-Chamorro, Frédéric Bonhomme, Anton Lukas Ipsen Wolff, Mathieu Stouf, François Lecointe, Marcel Hollenstein, Mart Krupovic, Marianne De Paepe, Yuvaraj Bhoobalan-ChittyPCR (polymerase chain reaction) fragments were fused together either by Overlap Extension-PCR or by Gibson Assembly (NEB #E2611S) [33] and cloned into the plasmid pBAD24. In case of the positional swapping between α-gt and β-gt,

(6) Rhythmic protein condensation in the circadian regulation of glycogen metabolismiScienceMay 15, 2026Lily A. Burton, Gopal K. Pattanayak, Michael J. RustM0492S EcoRI-HF,New England Biolabs,Cat# R3101S Gibson Assembly Master Mix,New England Biolabs,Cat# E2611S "cOmplete, EDTA free cocktail tablets",Sigma-Aldrich,Cat# 11873580001 Glycogen from bovine liver Type

(7) Generation of precise and accurate engineered circRNAs using enzymatic ligationNucleic Acids ResMay 8, 2026Amrita Singh, Adela Dujsikova, Noah Mueller, Y Grace ChenT7 promoter overhang. The amplified products were cloned into pcDNA3.1 using Gibson assembly (NEB #E2611L). Plasmid sequences were confirmed by whole plasmid sequencing (Plasmidsaurus). Unmodified DNA templates

(8) An anaerobic pathogen rewires host metabolism to fuel oxidative growth in the inflamed gutCellApril 30, 2026Luisella Spiga, Ryan T. Fansler, Yifan Wu, Alexandra Grote, Madison Langford-Butler, Asia K. Miller, Maxwell Neal, Owen F. Hale, Deepanshu Singla, M. Wade Calcutt, et al.BacTiter-Glo Microbial Cell Viability Assay,Promega,G8230 Foxp3 / Transcription Factor Staining Buffer Set,Invitrogen,00-5523-00 Gibson Assembly Cloning Kit,NEB,E2611 Illumina DNA Prep kit,Illumina,20060060 KAPA HiFi Hotstart ReadyMix,Roche,KK2601 Lysing Matrix B,MP

(9) GerAB residues predicted to interact with water based on MD simulations mediate germinosome stability in Bacillus subtilis sporesFrontiers in MicrobiologyApril 29, 2026Longjiao Chen, Silke van Buuren, George Korza, Jocelyne Vreede, Peter Setlow, Stanley Brulinserted in plasmid vector pUC19 with the Gibson Assembly Master Mix kit (New England BioLabs, NEB # E2611S). Plasmid- based mutagenesis was carried out with the QuikChange Lightning Site-Directed Mutagenesis Kit

(10) Co-release of tick-borne encephalitis virus RNA and structural proteins via virus-like particles and extracellular vesiclesJournal of General VirologyApril 29, 2026Ivana Křížová, Anna Klimešová, Alžběta Dostálková, Jana Racková, Jiřina Kaufmanová, Radim Novotný, Matěj Danda, Ivana Matošević, Zdeněk Franta, Hana Tykalová, et al.Gibson Assembly was applied according to the manufacturer’s protocol (Gibson Assembly® Master Mix, NEB #E2611). Individual fragments have been amplified using primers listed in Table S1 and sequence-verified. TBEV

(11) PhoP-regulated VirK acts as an accessory factor to maintain virulence in polymyxin-resistant Klebsiella pneumoniaeNucleic Acids ResApril 25, 2026Haibin Li, Longyang Jin, Penghe Wang, Ruobing Wang, Qi Wang, Xiukun Wang, Congran Li, Xuefu You, Hui WangpTOPO-P-R (Supplementary Table S1). The assembly was conducted using Gibson Assembly Master Mix (Cat. E2611S, NEB, Gene Company, China) according to the manufacturer’s instructions, and transformed into electrocompetent

(12) From Sea to Therapy: Development and Analytical Control of Recombinant Human CDKL5 Production in the Marine Bacterium Pseudoalteromonas haloplanktis TAC125Marine DrugsApril 24, 2026Andrea Coletti, Marzia Calvanese, Flora Cozzolino, Ilaria Iacobucci, Concetta Lauro, Angelica Severino, Maria Monti, Ermenegilda Parrilli, Maria Luisa Tutinoassembled according to the one-step isothermal DNA assembly method using Gibson assembly (NEB, cat #E2611) described by Gibson et al. (2009) [50]. To validate that the assembly was successful, the expression

(13) TraN variants mediate conjugation species specificity of IncA/C, IncH, and Acinetobacter baumannii plasmidsJournal of BacteriologyApril 23, 2026Shan He, Sophia David, Jaie Rattle, Julia Sanchez-Garrido, Wen Wen Low, Joshua L. C. Wong, Konstantinos Beis, Gad Frankelusing the same methodology. Mutagenesis vectors were generated by Gibson Assembly (New England Biolabs, E2611L) using the pSEVA612S backbone and were maintained in E. coli CC118λpir cells. Site-directed mutagenesis

(14) PEX11 mediates intralumenal vesicle formation in peroxisomesNat CommunApril 21, 2026Nathan E. Tharp, Chelsea An, James Hwang, Nayeli S. Shad, Zachary J. Wright, Bonnie Bartel(downstream of the HA tag) of the trifluorescent construct via Gibson assembly (New England Biolabs, E2611). The resultant plasmids were digested with XhoI and religated with Quick Ligase (New England Biolabs,

(15) T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signallingThe EMBO JournalApril 21, 2026Carmen Rodilla-Ramirez, Giorgia Carai, Eleanor Fox, Amin Zehtabian, Helen Adam, Katja Dallio, Pia Lazki-Hagenbach, Helge Ewers, Xiaolei Su, Francesca BottanelliSerum (FBS),Corning,35-079-CV Geneticin disulfate (G418),Roth,CP11.3 Gibson Assembly Master Mix,NEB,E2611 Glycerin,Roth,3783.1 GlutaMAX,Gibco,35050061 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES),Roth,9105.2 Human

(16) Pseudomonas aeruginosa deploys competitor-specific antagonistic strategiesbioRxivApril 20, 2026Ziyang Chen, Ned S. Wingreen, Zemer GitaiAllele exchange constructs were assembled by Gibson assembly using Gibson Assembly Master Mix (NEB, cat# E2611) and electroporated into electrocompetent E. coli S17. Positive S17 transformants were selected, plasmids

(17) Dimorphic neural network architecture prioritizes sexual-related behaviors in male Caenorhabditis elegansElifeApril 20, 2026Xuebin Wang, Hanzhang Liu, Wenjing Yang, Jingxuan Yang, Xuehong Sun, Qiuhan Liu, Ying Zhu, Yinghao Sun, Chunxiuzi Liu, Guiyuan Shi, et al.constructed via Gibson assembly (Gibson et al., 2009), utilizing the Gibson Assembly Master Mix (NEB, E2611) according to the manufacturer’s protocol. Gibson assembly is an isothermal, single-reaction method

(18) Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cellsCellApril 20, 2026Ujjwal Rathore, Eli Dugan, Hunter Thornton, Vigneshwari Easwar Kumar, Rama Dajani, Ryan C. Burdick, Janet M. Young, Zachary Steinhart, Reanna Lao, Krista A. Delviks-Frankenberry, et al.Dynabeads,Thermo Fisher Scientific,Cat# 40203D Gibson Assembly Master Mix - 50 rxns,New England Biolabs,Cat# E2611L GlycoBlue Coprecipitant (15 mg/mL),Thermo Fisher Scientific,Cat# AM9516 Invitrogen Recombinant Proteinase

(19) Dimorphic neural network architecture prioritizes sexual-related behaviors in male Caenorhabditis elegansElifeApril 20, 2026Xuebin Wang, Hanzhang Liu, Wenjing Yang, Jingxuan Yang, Xuehong Sun, Qiuhan Liu, Ying Zhu, Yinghao Sun, Chunxiuzi Liu, Guiyuan Shi, et al.constructed via Gibson assembly (Gibson et al., 2009), utilizing the Gibson Assembly Master Mix (NEB, E2611) according to the manufacturer’s protocol. Gibson assembly is an isothermal, single-reaction method

(20) Force-modulated structural landscape of the catch bonding F-actin crosslinker α-actinin-4bioRxivApril 16, 2026Alfred C. Chin, Fatemah Mukadum, Matthew J. Reynolds, Glen M. Hocky, Gregory M. AlushinPlasmid assembly and site-directed mutagenesis were performed using Gibson assembly (New England Biolabs, E2611L) or kinase-ligase-DpnI (KLD) cloning (New England Biolabs, M0554S). All plasmids were cloned and propagated

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Gibson Assembly Master Mix

Gibson Assembly Master Mix from New England Biolabs

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Gibson Assembly Master Mix from New England Biolabs

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