E.coli Ribosome P0763S from New England Biolabs

Product Specs
ItemE.coli Ribosome
CompanyNew England Biolabs
Price $201.00 Inquire
Catalog NumberP0763S
Size1 mg
ApplicationsProteomics & Glycomics
TypeProteins
SpeciesE.coli
Temperature-80 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducensNucleic Acids ResApril 24, 2026Benjamin F Jepson, Eric J Wolf, Anthony R Dawson, Jingmin Jin, Vladimir Potapov, Nan Dai, Ivan R Corrêa Jr., Erbay Yigitthe manufacturer’s instructions. Escherichia coli rRNA was purified from E. coli 70S ribosomes (NEB, P0763S) using the Monarch® RNA Cleanup Kit (NEB, T2050) following the manufacturer’s protocol. Digestion of

(2) RNA-specific local translation is patterned by condensates for multinucleate cell growthNat Cell BiolMarch 2, 2026Zachary M. Geisterfer, Ameya P. Jalihal, Sierra J. Cole, Amy S. Gladfelterused on the day of passivation. Labelling ribosomes for in vitro reactions E. coli ribosomes (NEB, P0763S) were labelled with Atto 565 NHS ester (Supelco, 72464) by combining the whole ribosome aliquot with

(3) Passage of ribosomes through micro-sprayer increases functional activity - Implications for activity assays in time-resolved cryo-EMJournal of Structural BiologySeptember 1, 2025Priyanka Garg, Xiangsong Feng, Swastik De, Joachim Frankthe large 50S and small 30S subunits, was obtained from New England Biolabs (NEB) (catalogue number P0763S) with an initial concentration of 13.3 μM. The sample was diluted to 7.2 μM using a buffer solution

(4) A hollow TFG condensate spatially compartmentalizes the early secretory pathwayNat CommunApril 19, 2025Savannah M. Bogus, William R. Wegeng, Miguel Ruiz, Sindy R. Chavez, Samantha N. Cheung, Khalid S. M. Noori, Ingrid R. Niesman, Andreas M. Ernstspecies were added at a final concentration of 1 µM and incubated for 10 min. Ribosomes (NEB, cat# P0763S) were labeled with NHS-SiR in approximately a 1:4 molar ratio, washed with 4 mL 50 mM HEPES/KOH, pH

(5) Remodelled ribosomal populations synthesize a specific proteome in proliferating plant tissue during coldPhilos Trans R Soc Lond B Biol SciMarch 6, 2025Federico Martinez-Seidel, Pipob Suwanchaikasem, Dione Gentry-Torfer, Yogeswari Rajarathinam, Alina Ebert, Alexander Erban, Alexandre Firmino, Shuai Nie, Michael Leeming, Nicholas Williamson, et al.the bicinchoninic acid kit (Thermo Scientific, United States) assay. As a control E. coli ribosomes (P0763S, NEB, Australia) were used in 4 µl aliquots (approx. 2000 A260 units that are equivalent to 102 µg of

(6) Resolving chaperone-assisted protein folding on the ribosome at the peptide levelNature Structural & Molecular BiologyDecember 1, 2024Thomas E. Wales, Aleksandra Pajak, Alžběta Roeselová, Santosh Shivakumaraswamy, Steven Howell, Svend Kjær, F. Ulrich Hartl, John R. Engen, David BalchinmM Tris-HCL, pH 7.5, 150 mM NaCl, 5% glycerol and 1 mM DTT; for free ribosomes (obtained from NEB, P0763S), 20 mM HEPES-KOH, pH 7.6, 10 mM Mg(OAc)2, 30 mM KCl and 7 mM β-mercaptoethanol. Proteins were diluted,

(7) Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effectsNature MethodsJune 1, 2024Zi Yang, Jingjin Fan, Jia Wang, Xiao Fan, Zheng Ouyang, Hong-Wei Wang, Xiaoyu Zhouspecimen preparation The 70S ribosomes and streptavidin were purchased from the NEB company (catalog nos. P0763S and N7021S), while Thermoplasma acidophilum 20S proteasomes49, human heavy-chain apo-ferritins50 and

(8) Streamlining Protein Fractional Synthesis Rates Using SP3 Beads and Stable Isotope Mass Spectrometry: A Case Study on the Plant RibosomeBIO-PROTOCOLMay 5, 2024Dione Gentry Torfer, Ester Murillo, Chloe L. Barrington, Shuai Nie, Michael G. Leeming, Pipob Suwanchaikasem, Nicholas A. Williamson, Ute Roessner, Berin A. Boughton, Joachim Kopka, et al.previous studies [36] (Note 1) Escherichia coli 70S ribosomes (New England BioLabs, catalog number: P0763S); one tube of 1 mg is enough as internal standard for the protocol detailed below Reagents Guanidine hydrochloride

(9) Graphene sandwich–based biological specimen preparation for cryo-EM analysisProceedings of the National Academy of SciencesJanuary 30, 2024Jie Xu, Xiaoyin Gao, Liming Zheng, Xia Jia, Kui Xu, Yuwei Ma, Xiaoding Wei, Nan Liu, Hailin Peng, Hong-Wei Wangsandwich. Preparation of Biological Samples. We purchased ribosomes from New England Biolabs (Catalog: P0763S) and diluted them to 1.11 mg/mL for use. Apoferritin was purchased from Sigma-Aldrich (Catalog: A3660)

(10) Next-generation proteomics for quantitative Jumbophage-bacteria interaction mappingNat CommunAugust 24, 2023Andrea Fossati, Deepto Mozumdar, Claire Kokontis, Melissa Mèndez-Moran, Eliza Nieweglowska, Adrian Pelin, Yuping Li, Baron Guo, Nevan J. Krogan, David A. Agard, et al.estimated using a protein mixture (Phenomenex AL0-3042), while an E. coli 70s ribosome (NEB, cat nr P0763S) was used to estimate which fractions to use for ribosome XL-MS. SEC-MS proteomics sample preparation The

(11) Attachment of the RNA degradosome to the bacterial inner cytoplasmic membrane prevents wasteful degradation of rRNA in ribosome assembly intermediatesPLOS BiologyJanuary 5, 2023Lydia Hadjeras, Marie Bouvier, Isabelle Canal, Leonora Poljak, Quentin Morin-Ogier, Carine Froment, Odile Burlet-Schlitz, Lina Hamouche, Laurence Girbal, Muriel Cocaign-Bousquet, et al.coefficient calculated from the amino acid composition of the protein. Ribosomes were purchased from NEB (P0763S). Ribosomal RNA was prepared by extraction of ribosomes using a Direct-zol RNA purification kit. The

(12) Towards sub-millisecond cryo-EM grid preparationFaraday DiscussionsNovember 8, 2022David P. Klebl, Robert W. Kay, Frank Sobott, Nikil Kapur, Stephen P. Muenchglycerol, 1 mM DTT pH 7.5. The Escherichia coli ribosome sample was purchased from New England Biolabs (P0763S). For grid preparation, the sample was diluted to 6.6 mg mL−1 in 50 mM HEPES, 8 mM MgCl2, 100 mM potassium

(13) Functionalized graphene grids with various charges for single-particle cryo-EMNat CommunNovember 7, 2022Ye Lu, Nan Liu, Yongbo Liu, Liming Zheng, Junhao Yang, Jia Wang, Xia Jia, Qinru Zi, Hailin Peng, Yu Rao, et al.ultracentrifugation to get purified Ll.LtrB RNP sample. E. Coli ribosome was purchased from NEB company (P0763S). Cryo-EM specimen preparation For sample vitrification, we firstly pipetted 7 μl sample buffer (100

(14) Bacterial surface interactions with organic colloidal particles: Nanoscale hotspots of organic matter in the oceanPLOS ONEAugust 25, 2022Nirav Patel, Ryan Guillemette, Ratnesh Lal, Farooq Azamin each condition included the following: 0.3 or 3.3 μg E. coli ribosomes (New England BioLabs Cat# P0763S, Ipswich, MA USA) mL-1 culture (hereafter “R”); ribosome buffer (final concentration = 2 μM Hepes-KOH,

(15) Need for Speed: Examining Protein Behaviour During CryoEM Grid Preparation at Different TimescalesSSRN Electronic JournalJune 17, 2022David P. Klebl et al.Recombinant Proteins Horse Spleen apoferritin Sigma Aldrich A3660 E. coli ribosome New England Biolabs P0763S IPTG Sigma Aldrich 367-93-1 Protease inhibitor cocktail Calbiochem 539-134 Ni-NTA affinity Resin Generon NB-45-00042 10kDa

(16) Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root MeristemsFrontiers in Plant ScienceApril 30, 2021Federico Martinez-Seidel, Pipob Suwanchaikasem, Shuai Nie, Michael G. Leeming, Alexandre Augusto Pereira Firmino, Nicholas A. Williamson, Joachim Kopka, Ute Roessner, Berin A. BoughtonmM of chloramphenicol, 0.18 mM of cycloheximide, and 5 mM of DTT. Pure Escherichia coli ribosomes (P0763S, NEB, Australia) were ultracentrifuged following the same procedure as a control of the ribosomal particles

(17) Simplified Approach for Preparing Graphene Oxide TEM Grids for Stained and Vitrified BiomoleculesNanomaterials (Basel)March 5, 2021Anil Kumar, Nayanika Sengupta, Somnath Duttadirectly used for negative staining. Three different biological samples, E. coli 70S Ribosome (NEB P0763S), Vibrio cholerae Cytolysin and MdtB from E. coli were visualized using this GO-coated grids. Standard

(18) Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different TimescalesStructureNovember 3, 2020David P. Klebl et al.in 30 mM HEPES, 150 mM NaCl pH 7.5. E. coli ribosome sample was purchased from New England Biolabs (P0763S), provided at a stock concentration of 33 mg/mL (= approx. 25 μM assuming an average molecular weight

(19) The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infectionPLOS ONEOctober 1, 2020Fernando Sorroche et al.(wt). https://doi.org/10.1371/journal.pone.0235446.g005 nodules, as well as a commercial preparation of E. coli ribosomes (NEB P0763S), displayed no or little signal activity, in contrast to RNAse A-dissociated ribosomes (Fig 6). Mass spectrometry analysis

(20) Synthetic group A streptogramin antibiotics that overcome Vat resistanceNatureOctober 1, 2020Qi Li et al.the PURExpress® Δ Ribosome Kit (E3313, NEB) for in vitro protein synthesis, 70S E. coli ribosomes (P0763S, NEB), murine RNAse inhibitor (M0314, NEB), and 6.66 ng/μl of template DNA encoding the fluorescent

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E.coli Ribosome

E.coli Ribosome from New England Biolabs

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E.coli Ribosome from New England Biolabs

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