Bacteroides Heparinase II P0736L from New England Biolabs

Product Specs
ItemBacteroides Heparinase II
CompanyNew England Biolabs
Price $517.00 Inquire
Catalog NumberP0736L
Size200 units
ApplicationsProteomics & Glycomics
Temperature-80 C
TypeGlycosidases

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Novel regulators of heparan sulfate proteoglycans modulate cellular uptake of α-synuclein fibrilsCommun BiolOctober 6, 2025Benoît Vanderperre, Amitha Muraleedharan, Marie-France Dorion, Frédérique Larroquette, Esther Del Cid Pellitero, Nishani Rajakulendran, Carol X.-Q. Chen, Roxanne Larivière, Charlotte Michaud-Tardif, Thomas Goiran, et al.h, or for 1 h in serum-free DMEM with a cocktail of heparinase I (NEB, P0735S), heparinase II (NEB, P0736S) and heparinase III (NEB, P0737S) at 3 U/mL each. GFP+ binding assay was performed as for PFFs binding

(2) Nanoscopy Reveals Heparan Sulfate Clusters as Docking Sites for SARS-CoV-2 Attachment and EntrybioRxivSeptember 16, 2025Sue Han, Xin Wang, Tiansheng Li, Ammar Mohseni, Ivan Kosik, Chung Yu Chan, Alberto Domingo López-Muñoz, Jessica Matthias, Reid Suddaby, Zhixiong Wang, et al.heparinase I/II/III mixture containing heparinase I (3.2 units, NEB, #P0735S), heparinase II (1 units, NEB, #P0736S) and heparinase III (0.19 units, NEB, #P0737S), was applied to cells in FBS-free DMEM at 30°C for 1

(3) The Transpeptidase Sortase A Binds Nucleic Acids and Mediates Mammalian Cell LabelingAdvanced ScienceJune 1, 2024Yingzheng Liu, Zhike Lu, Panfeng Wu, Zhaohui Liang, Zhenxing Yu, Ke Ni, Lijia Mamillion cells were counted and treated with glycosidases (Heparinase I NEB P0735S, Heparinase II NEB P0736L, Heparinase III NEB P0737S, Chondroitinase ABC Sigma C3667, PNGase F NEB P0705S, O‐Glycosidase NEB P0733S,

(4) Isolation and Compositional Analysis of GlycosaminoglycansMethods in Molecular BiologyMay 1, 2024Stephanie Archer-Hartmann, Lauren E. Pepi, Christian Heiss, Parastoo AzadiHeparinase enzymes can be purchased from a number of vendor sources including New England Biolabs (P0735S, P0736S, P0737S), Sigma (H2519, H6512, H8891), GrampEnz (GE-H0001, GE-H0002, GE-H0003), or IBEX (60–010, 60–018,

(5) Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cellsCell reports. MedicineApril 16, 2024Albanese M, Chen HR, Gapp M, Muenchhoff M, Yang HH, Peterhoff D, Hoffmann K, Xiao Q, Ruhle A, Ambiel I, et al.Aldrich,Cat#A6964-100ML Heparinase I,New England Biolabs,Cat#P0735S Heparinase II,New England Biolabs,Cat#P0736S Heparinase III,New England Biolabs,Cat#P0737S Chondroitinase ABC from Proteus vulgaris,Merck,Cat#C3667-5UN Lipopolysaccharide

(6) Dynamic Changes in Heparan Sulfate Nanostructure in Human Pluripotent Stem Cell DifferentiationACS NanoApril 25, 2023Deena Al Al Mahbuba, Sayaka Masuko, Shiwei Wang, Deepsing Syangtan, Jeong Seuk Kang, Yuefan Song, Tay Won Shin, Ke Xia, Fuming Zhang, Robert J. Linhardt, et al.composed of Bacteroides heparinase I (New England Biolabs, #P0735), heparinase II (New England Biolabs, #P0736), and heparinase III (New England Biolabs, #P0737). Each enzyme was used at 4 unit/ml concentration

(7) Cell Surface Nucleocapsid Protein Expression: A Betacoronavirus Immunomodulatory StrategybioRxiv : the preprint server for biologyFebruary 27, 2023López-Muñoz AD, Santos JJS, Yewdell JW.treated with Bacteroides heparinase I (4.8 units), II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C. Cells were washed twice, incubated with recombinant proteins, washed,

(8) Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunityScience advancesAugust 5, 2022López-Muñoz AD, Kosik I, Holly J, Yewdell JW.with Bacteroides heparinase I (4.8 U), II (1.6 U), and III (0.28 U) (New England Biolabs, nos. P0735S, P0736S, and P0737S) in DPBS for 1 hour at 30°C. Cells were washed twice, incubated with recombinant proteins,

(9) Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive ImmunityResearch squareDecember 13, 2021Yewdell J, Lopez-Munoz A, Kosik I, Holly J.treated with Bacteroides heparinase I (4.8 units), II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C. Cells were washed twice, incubated with recombinant proteins, and

(10) Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive ImmunitybioRxiv : the preprint server for biologyDecember 13, 2021López-Muñoz AD, Kosik I, Holly J, Yewdell JW.treated with Bacteroides heparinase I (4.8 units), II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C. Cells were washed twice, incubated with recombinant proteins, and

(11) Evaluation and Refinement of Sample Preparation Methods for Extracellular Matrix Proteome CoverageMolecular & Cellular ProteomicsJune 3, 2021Maxwell C. McCabe, Lauren R. Schmitt, Ryan C. Hill, Monika Dzieciatkowska, Mark Maslanka, Willeke F. Daamen, Toin H. van Kuppevelt, Danique J. Hof, Kirk C. HansenRemoval Removal of N-linked glycans and glycosaminoglycans (GAGs) using PNGaseF (NEB #P0704), heparinase II (NEB #P0736), and chondroitinase ABC (Sigma-Aldrich #C3667) was performed across multiple digestion steps. Filter

(12) Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in ObesityCell metabolismFebruary 2, 2021Brestoff JR, Wilen CB, Moley JR, Li Y, Zou W, Malvin NP, Rowen MN, Saunders BT, Ma H, Mack MR, et al.Heparan sulfate proteoglycan,Sigma-Aldrich,Cat#H4777 Heparanase I,New England Biolabs,Cat#P0735S Heparanase II,New England Biolabs,Cat#P0736L Heparanase III,New England Biolabs,Cat#P0737L Humulin R,Lilly,Cat#0002-8215-17 L-glutamine,Corning,Cat#25-005-Cl "Lipopolysaccharide

(13) Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysisMolecular OmicsApril 21, 2020Manveen K. Sethi, Margaret Downs, Joseph ZaiaScientific) Hyaluronidase (H1136-1AMP, Sigma) Chondroitinase ABC (Sigma) Heparan lyases 1,2,3 (P0735L, P0736L, P0737L New England Biolabs) Trypsin (PRV5280, Promega)/ Trypsin-LysC (PRV5072, Promega)/ Glu-C (PRV1651,

(14) On-slide tissue digestion for mass spectrometry based glycomic and proteomic profilingMethodsXSeptember 25, 2019Rekha Raghunathan, Manveen K. Sethi, Joseph Zaiaglacial, JT Baker)Hyaluronidase (H1136-1AMP, Sigma)Chondroitinase ABC(Sigma)Heparan lyases 1,2,3(P0735L, P0736L, P0737L New England Biolabs)PNGase F(P0705L, New England Biolabs)Trypsin (V528A, Prozyme)Kasil (C033116,

(15) Heparan Sulfate Organizes Neuronal Synapses through Neurexin PartnershipsCellSeptember 6, 2018Zhang P, Lu H, Peixoto RT, Pines MK, Ge Y, Oku S, Siddiqui TJ, Xie Y, Wu W, Archer-Hartmann S, et al.stopped by heating to 100°C for five minutes. 1μL each of Bacteroides Heparinases I, II, and III (P0735L, P0736L, P0737L New England Biolabs) were then added to the sample and the mixture was digested at 37°C for

(16) Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosisPhysiological reportsJuly 1, 2017Kijani S, Vázquez AM, Levin M, Borén J, Fogelstrand P.experiments, tissue sections were pre‐incubated 30 min at RT with Heparinase II (New England BioLabs, P0736S) or Chondrotinase ABC (Sigma, C3667) as per manufacturer's recommendations. After pre‐incubations, tissue

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Bacteroides Heparinase II

Bacteroides Heparinase II from New England Biolabs

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Bacteroides Heparinase II from New England Biolabs

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