LongAmpHot StartTaqDNA Polymerase M0534S from New England Biolabs

Product Specs
ItemLongAmpHot StartTaqDNA Polymerase
CompanyNew England Biolabs
Price $122.00 Inquire
Catalog NumberM0534S
Size500 units
ApplicationsDNA Analysis
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) HIV-1 virome profiling using HIV-PULSE to guide therapeutic and curative interventionsEBioMedicineMay 21, 2026Sofie De Braekeleer, Laurens Lambrechts, Liesbet Termote, Mareva Delporte, Kavita Mehta, Vasiliki Matzaraki, Evy E. Blomme, Philippe Lemey, Bram Vrancken, Casper Rokx, et al.all the cleaned pre-amplified product (30 μL), 2 μL of LongAmp Hot Start Taq DNA Polymerase (NEB, #M0534), 0.5 μM of each primer (Second PCR F UMI, Second PCR R UMI), 1.5 μL of 10 mM dNTPs (Promega, #C1141),

(2) Microbial and inflammatory profiling of pressure injuries and urinary tract infections in spinal cord injury: a prospective cohort studyScientific ReportsMarch 25, 2026Alessandro Bertolo, Reto Wettstein, Ezra Valido, Simona Capossela, Joel Buergin, Alexander Haumer, Nicole Speck, Nicole Nyfeler, Alexandra Widmer, Juergen Pannek, et al.universal primers (400 nM), with 5 ng of gDNA and 12.5 µL of LongAmp DNA Polymerase (New England Biolabs, M0534) in a final volume of 25 µL. Specific products were amplified using a Real-Time PCR system (Bio-Rad

(3) Bioaugmentation with native Bacillus strains enhance nitrate and nitrite removal and reshape microbiomes in low-salinity shrimp cultures: Elucidating genetic mechanismsPLOS OneJanuary 9, 2026Dámaris Adelaida Esquén Bayona, Delia Talledo Ancajima, Dorian Adriano Cadena, Luz Dominguez-Mendoza, Sebastian Leyva, Mia Mariana Somocurcio Zambrano, David Edilberto Saldarriaga Yacila, Pabulo Henrique Rampelotto, Frank Lino Guzman Escudero, Benoit Mathieu DiringerrRNA gene from 20ng of input DNA using PCR with LongAmp Hot Start Taq DNA Polymerase 1X Master Mix (M0534S, NEB, USA) [33]. On the other hand, the DNA collected on day 10 in CO and treatment T2 was used to prepare

(4) Efficacious genome editing in infant mice with glycogen storage disease type IaJCI InsightSeptember 23, 2025Benjamin Arnson, Ekaterina Ilich, Troy von Beck, Songtao Li, Elizabeth D. Brooks, Dorothy Gheorghiu, Gordon He, Matthew Weinrub, Sze Ying Chan, Hye-Ri Kang, et al.PCR reactions were performed with LongAmp Hot Start Taq DNA Polymerase (New England Biolabs, catalog M0534L) according to the manufacturer’s recommendations, with a 65°C annealing temperature and 18-minute extension

(5) A long noncoding RNA with enhancer-like function in pig zygotic genome activationJournal of Molecular Cell BiologyJune 28, 2025Renyue Wei, Yanbin Yue, Yinhuan Wu, Chenyuan Zhang, Jun-Xue Jin, Zhonghua Liu, Jiaqiang Wang(Addgene, 61422) and the plasmid MS2-P65-HSF1_GFP (Addgene, 61423) using LongAmpTM Taq DNA Polymerase (NEB, M0534L), respectively. Then, in vitro transcription was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis

(6) Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technologyBMC MicrobiologyFebruary 16, 2024Alessandro Bertolo, Ezra Valido, Jivko Stoyanovpolymerases were used: LongAmp® Hot Start Taq DNA Polymerase (LongAmp DNA Polymerase, New England Biolabs, M0534) and iQ SYBR® Green Supermix (iTaq DNA Polymerase, Bio-Rad, 1708880), both supplied with a ready-to-use

(7) Whole-genome Comparisons Identify Repeated Regulatory Changes Underlying Convergent Appendage Evolution in Diverse Fish LineagesMolecular Biology and EvolutionSeptember 22, 2023Heidi I Chen, Yatish Turakhia, Gill Bejerano, David M Kingsley(New England Biolabs catalog # M0531), and/or LongAmp® Hot Start Taq DNA Polymerase (New England Biolabs catalog # M0534S) with Expand HF Buffer (Roche catalog # 05917131103) according to manufacturer recommendations. The

(8) Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editingScience AdvancesOctober 21, 2022So Hyun Park, Mingming Cao, Yidan Pan, Timothy H. Davis, Lavanya Saxena, Harshavardhan Deshmukh, Yilei Fu, Todd Treangen, Vivien A. Sheehan, Gang Baocenter was amplified using L-R PCR [LongAmp Hot Start Taq DNA Polymerase; New England Biolabs (NEB), M0534S]. L-R PCR amplicons were run on an agarose gel to check for the gel shift indicating LD. The presence

(9) Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutationsPLOS ONEDecember 23, 2020Jennifer L. Steele, Richard C. Stevens, Oscar A. Cabrera, Gary J. Bassill, Sabrina M. Cramer, Felipe Guzman, Anthony P. Shuberwas performed using a universal primer (S2 Table) and LongAmp Hot Start Taq Polymerase (NEB, cat. #M0534L) with an annealing temperature of 65˚C. After amplification, DNA was purified using AMPure XP beads

(10) Nucleotide polymorphism assay for the identification of west African group Bacillus anthracis: a lineage lacking anthroseBMC MicrobiologyDecember 1, 2020Diansy Zincke, Michael H. Norris, Berzhan Kurmanov, Ted L. Hadfield, Jason K. Blackburneach primer, 5 μl of 5X LongAmp Taq Buffer and 2.5 U of LongAmp Hot Start Taq DNA Polymerase (NEB, M0534S). An initial denaturation at 94 °C for 30 s was followed by 30 cycles of 94 °C for 20 s, gradient annealing

(11) EcoTILLING by sequencing reveals polymorphisms in genes encoding starch synthases that are associated with low glycemic response in riceBMC Plant BiologyDecember 1, 2017Ramadoss Bharathi Raja, Somanath Agasimani, Sarita Jaiswal, Venkatesan Thiruvengadam, Robin Sabariappan, Ravindra N. Chibbar, Sundaram Ganesh RamS2). PCR for amplification of EcoTILLING fragments High fidelity LongAmp® Taq DNA polymerase (Cat#M0534) obtained from New England Biolabs (NEB), Ipswich, UK was used for PCR amplification in a 50 μl reaction.

(12) A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two‐cell embryosEMBO reportsOctober 1, 2016Jiaqiang Wang et al.Reverse Transcription Kit (ABI, #4368814). PCR was performed by LongAmpTM Taq DNA polymerase (NEB, #M0534L). TM-qPCR was performed by TaqMan Universal Master Mix II (Life, #4440048). 50 -RACE was performed by

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LongAmpHot StartTaqDNA Polymerase

LongAmpHot StartTaqDNA Polymerase from New England Biolabs

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LongAmpHot StartTaqDNA Polymerase from New England Biolabs

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