BstEII-HF R3162M from New England Biolabs

Product Specs
ItemBstEII-HF
CompanyNew England Biolabs
Price $281.00 Inquire
Catalog NumberR3162M
Size10000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) High‐Throughput Isolation of Nanomouse‐Derived VHH Domains: A Practical Guide from Immunization to Nanobody ExpressionCurr ProtocJuly 21, 2026Tessa J. Casselman, Asa W. Huffaker, Kristie C. Mitchell, Jamie E. Schnarrs, Sylvia Ni, Mary E. Skinner, Matthias C. TruttmannrCutSmart buffer (New England Biolabs, cat. no. B6004S)BstEII‐HF restriction enzyme (New England Biolabs, cat. no. R3162S)PstI restriction enzyme (New England Biolabs, cat. no. R0140S) UltraPure nuclease‐free water (Invitrogen,

(2) Unrolling worms: Genome Editing to Rewrite Roller Phenotypes in C. elegansMicroPubl BiolJune 30, 2026Sohitri Mukherjee, Andrea Kh StavoeCCC TAA CTA CGG rol-6 ( su1006 ) Rev: GAA TGG ACC ATC TGG GAA TCC ACC AatII (New England Biolabs # R3162S) cuts N2 (421 and 31 bp) and rol-6 ( su1006 ) (242, 179 and 31 bp). 1kb plus DNA ladder (New England

(3) MICU proteins facilitate calcium-dependent mitochondrial metabolon formation to regulate cellular energetics independently of MCUNature MetabolismMay 13, 2026Henry M. Cohen, Benjamin Gottschalk, Carmen Choya-Foces, Adam Chatoff, Anya Wilkinson, Elena Berezhnaya, Joanne F. Garbincius, Adyson Johnson, Tyler L. Stevens, Jordan E. Howe, et al.by cutting MICU3 and MICU3EFmut out of their respective vectors using BstEII (New England Biolabs, R3162S) and MfeI (New England Biolabs, R3589S) and ligating the mutant gene fragment into the WT vector. MICU1EFmut/MICU2EFmut/MICU3

(4) Nanobody Immunolabelling and three-dimensional imaging reveals spatially restricted LYVE1 expression by kidney lymphatic vessels in miceJ NanobiotechnologyOctober 13, 2025Eva Maria Funk, Daniyal J. Jafree, Nils Rouven Hansmeier, Clàudia Abad Baucells, Rose Yinghan Behncke, Gideon Pomeranz, Maria Kolatsi-Joannou, William J. Mason, Dale Moulding, Lauren G. Russell, et al.digested for 20 min at 37 °C with PstI-HF (R3140, New England Biolabs, Ipswich, MA, USA) and BstEII-HF (R3162, New England Biolabs) restriction enzymes, while empty pHEN6c plasmids were concurrently digested with

(5) Systematic genetic characterization of the human PKR kinase domain highlights its functional malleability to escape a poxvirus substrate mimicElifeNovember 12, 2024Michael James Chambers, Sophia B Scobell, Meru J Sadhupurification. MSp508_MCS was digested with restriction enzymes AgeI-HF (NEB Cat#R3552S) and BstEII-HF (NEB Cat#R3162S), followed by gel purification. The digested MSp508_MCS and pGAL10/CYC1-PKR fragments were joined via

(6) Systematic genetic characterization of the human PKR kinase domain highlights its functional malleability to escape a poxvirus substrate mimiceLifeNovember 12, 2024Michael James Chambers, Sophia B Scobell, Meru J Sadhupurification. MSp508_MCS was digested with restriction enzymes AgeI-HF (NEB Cat#R3552S) and BstEII-HF (NEB Cat#R3162S), followed by gel purification. The digested MSp508_MCS and pGAL10/CYC1-PKR fragments were joined via

(7) Systematic characterization of the local evolutionary space available to human PKR and vaccinia virus K3bioRxivNovember 25, 2023Michael J. Chambers, Sophia Scobell, Meru J. Sadhupurification. YCp50-RD was digested with restriction enzymes AgeI-HF (NEB Cat#R3552S) and BstEII-HF (NEB Cat#R3162S), followed by gel purification. The digested YCp50-RD and pGAL/CYC1-PKR fragments were joined via Gibson

(8) Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafishDevelopmental CellNovember 1, 2023Masahiro Shin, Hui-Min Yin, Yu-Huan Shih, Takayuki Nozaki, Daneal Portman, Benjamin Toles, Amy Kolb, Kevin Luk, Sumio Isogai, Kinji Ishida, et al.,New England Biolabs,R3104 EcoRI-HF,New England Biolabs,R3101 ScaI-HF,New England Biolabs,R3122 BstEII-HF,New England Biolabs,R3162 NcoI-HF,New England Biolabs,R3193 PacI,New England Biolabs,R0547 SspI-HF,New England Biolabs,R0132 BbsI-HF,New England Biolabs

(9) LRP8-mediated selenocysteine uptake is a targetable vulnerability in MYCN-amplified neuroblastomaEMBO molecular medicineAugust 7, 2023Alborzinia H, Chen Z, Yildiz U, Freitas FP, Vogel FCE, Varga JP, Batani J, Bartenhagen C, Schmitz W, Büchel G, et al.from the puromycin resistance gene. For this, the plasmid was linearized with BstEII‐HF (NEB, Cat. No. R3162) and MluI‐HF (NEB, Cat. No. R3198), and the synthetic dsDNA fragment (manufactured by Twist Bioscience,

(10) Protocol for the High-quality Plasmid Isolation from Different Recalcitrant Bacterial Species: Agrobacterium spp., Rhizobium sp., and Bacillus thuringiensisBio ProtocAugust 5, 2023Preshobha Kodackattumannil, Shina Sasi, Saranya Krishnan, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri34860-2.5L-R)NcoI-HF (NEB, catalog number: R3193M), storage at -20 °CBstEII-HF (NEB, catalog number: R3162M), storage at -20 °CHotStar Taq DNA Polymerase PCR kit (Qiagen, catalog number: 203205)PCR primers (Macrogen,

(11) Protocol for the High-quality Plasmid Isolation from Different Recalcitrant Bacterial Species: Agrobacterium spp., Rhizobium sp., and Bacillus thuringiensisBio ProtocAugust 5, 2023Preshobha Kodackattumannil, Shina Sasi, Saranya Krishnan, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri34860-2.5L-R)NcoI-HF (NEB, catalog number: R3193M), storage at -20 °CBstEII-HF (NEB, catalog number: R3162M), storage at -20 °CHotStar Taq DNA Polymerase PCR kit (Qiagen, catalog number: 203205)PCR primers (Macrogen,

(12) Elucidation of the binding mode of organic polysulfides on the human TRPA1 receptorFrontiers in PhysiologyJune 7, 2023Balázs Nemes, Szabolcs László, Balázs Zoltán Zsidó, Csaba Hetényi, Adam Feher, Ferenc Papp, Zoltan Varga, Éva Szőke, Zoltán Sándor, Erika PintérThe final PCR products and intact pT31-hTRPA1 plasmid were digested by BstEII-HF (New England Biolabs R3162) and BbvCI (New England Biolabs R0601) enzymes (Figure 2C), then isolated by agarose gel electrophoresis,

(13) 3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM)International journal of molecular sciencesApril 15, 2022Hansmeier NR, Büschlen IS, Behncke RY, Ulferts S, Bisoendial R, Hägerling R.PCR products were digested with PstI-HF (R3140, New England Biolabs, Ipswich, MA, USA) and BstEII-HF (R3162, New England Biolabs, Ipswich, MA, USA) restriction enzymes for 20 min at 37 °C. Simultaneously, empty

(14) Tighter αC-helix–αL16-helix interactions seem to make p38α less prone to activation by autophosphorylation than Hog1Bioscience ReportsApril 1, 2016Masha Tesker, Sadiduddin Edbe Selamat, Jonah Beenstock, Ruchama Hayouka, Oded Livnah, David Engelbergare listed in Table 2. Then, the DNA was cloned to the vector of interest, using the BstEII (NEB # R3162S) and BlpI (NEB # R0585S) restriction enzymes for HOG1 and the EcoRI (NEB #R3101S) restriction enzyme

Be the First to Write a Review!

BstEII-HF

BstEII-HF from New England Biolabs

New England Biolabs

Citations (14)

Reviews

BstEII-HF from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map