BacteroidesHeparinase I P0735S from New England Biolabs

Product Specs
ItemBacteroidesHeparinase I
CompanyNew England Biolabs
Price $172.00 Inquire
Catalog NumberP0735S
Size240 units
ApplicationsProteomics & Glycomics
Temperature-80 C
TypeGlycosidases

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

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Website: http://www.neb.com

(1) Novel regulators of heparan sulfate proteoglycans modulate cellular uptake of α-synuclein fibrilsCommun BiolOctober 6, 2025Benoît Vanderperre, Amitha Muraleedharan, Marie-France Dorion, Frédérique Larroquette, Esther Del Cid Pellitero, Nishani Rajakulendran, Carol X.-Q. Chen, Roxanne Larivière, Charlotte Michaud-Tardif, Thomas Goiran, et al.G) in complete medium for 48 h, or for 1 h in serum-free DMEM with a cocktail of heparinase I (NEB, P0735S), heparinase II (NEB, P0736S) and heparinase III (NEB, P0737S) at 3 U/mL each. GFP+ binding assay was

(2) Nanoscopy Reveals Heparan Sulfate Clusters as Docking Sites for SARS-CoV-2 Attachment and EntrybioRxivSeptember 16, 2025Sue Han, Xin Wang, Tiansheng Li, Ammar Mohseni, Ivan Kosik, Chung Yu Chan, Alberto Domingo López-Muñoz, Jessica Matthias, Reid Suddaby, Zhixiong Wang, et al.washed out. HPRase (35), the heparinase I/II/III mixture containing heparinase I (3.2 units, NEB, #P0735S), heparinase II (1 units, NEB, #P0736S) and heparinase III (0.19 units, NEB, #P0737S), was applied to

(3) Granular cytoplasmic inclusions in astrocytes and microglial activation in the fetal brain of pigtail macaques in response to maternal viral infectionActa Neuropathol CommunMarch 11, 2025Raj P. Kapur, Andrew E. Vo, Amanda Li, Miranda Li, Jeff Munson, Hazel Huang, Briana Del Rosario, Orlando Cervantes, Hong Zhao, Ashley Vong, et al.gene-specific primers or random primers. Serum or plasma was treated with Bacteroides Heparinase I (Cat# P0735S, New England Biolabs, Ipswich, MA, USA) after cDNA synthesis. Viral RNA was quantified using the TaqMan

(4) Multifocal, multiphenotypic tumours arising from an MTOR mutation acquired in early embryogenesisOncogeneOctober 28, 2024Clarissa N. Pacyna, Madhanagopal Anandapadamanaban, Kevin W. Loudon, Iain M. Hay, Olga Perisic, Ruoyan Li, Matthew Byrne, Laura Allen, Kirsty Roberts, Yvette Hooks, et al.by simultaneous overnight tag cleavage by TEV and dephosphorylation by λ-protein phosphatase (NEB, P0735). The cleaved, dephosphorylated protein was further purified by anion exchange chromatography on a 5

(5) The Transpeptidase Sortase A Binds Nucleic Acids and Mediates Mammalian Cell LabelingAdvanced ScienceJune 1, 2024Yingzheng Liu, Zhike Lu, Panfeng Wu, Zhaohui Liang, Zhenxing Yu, Ke Ni, Lijia Malabeling. A total of 0.5 million cells were counted and treated with glycosidases (Heparinase I NEB P0735S, Heparinase II NEB P0736L, Heparinase III NEB P0737S, Chondroitinase ABC Sigma C3667, PNGase F NEB P0705S,

(6) Isolation and Compositional Analysis of GlycosaminoglycansMethods in Molecular BiologyMay 1, 2024Stephanie Archer-Hartmann, Lauren E. Pepi, Christian Heiss, Parastoo AzadiHeparinase enzymes can be purchased from a number of vendor sources including New England Biolabs (P0735S, P0736S, P0737S), Sigma (H2519, H6512, H8891), GrampEnz (GE-H0001, GE-H0002, GE-H0003), or IBEX (60–010,

(7) Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusionsiScienceApril 19, 2024Ramella M, Ribolla LM, Surini S, Sala K, Tonoli D, Cioni JM, Rai AK, Pelkmans L, de Curtis I.phosphatase inhibitors. Dephosphorylation assays were performed with 5 units of l protein phosphatase (P0735S, New England Biolabs) per μg of protein lysate in 1× L phosphatase buffer (New England Biolabs buffer

(8) Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cellsCell reports. MedicineApril 16, 2024Albanese M, Chen HR, Gapp M, Muenchhoff M, Yang HH, Peterhoff D, Hoffmann K, Xiao Q, Ruhle A, Ambiel I, et al.Aldrich,Cat#04716728001 NLS-Cas9,IDT,Cat#1081059 Accutase,Sigma Aldrich,Cat#A6964-100ML Heparinase I,New England Biolabs,Cat#P0735S Heparinase II,New England Biolabs,Cat#P0736S Heparinase III,New England Biolabs,Cat#P0737S Chondroitinase

(9) Sustained hyperglycemia specifically targets translation of mRNAs for insulin secretionJournal of Clinical InvestigationFebruary 1, 2024Abigael Cheruiyot, Jennifer Hollister-Lock, Brooke A Sullivan, Hui Pan, Jonathan M. Dreyfuss, Susan Bonner-Weir, Jean E. Schaffer(Zymo Research). RNA recovered from sucrose density gradient fractions was treated with 600 U/mL heparinase (NEB P0735S; 20 minutes, room temperature [RT]). Five hundred nanograms to 1 μg RNA was reverse-transcribed using

(10) Non-fatal outcomes of COVID-19 disease in pediatric organ transplantation associates with down-regulation of senescence pathwaysScientific ReportsJanuary 22, 2024Kumar Subramanian, Rency Varghese, Molly Pochedly, Vinona Muralidaran, Nada Yazigi, Stuart Kaufman, Khalid Khan, Bernadette Vitola, Alexander Kroemer, Thomas Fishbein, et al.described previously52–54. Briefly, 10 μL of RNA was added to 0.5 μL of heparinase 1 from Bacteroides (NEB, P0735S), 1 μL RNase inhibitor ((Enzymatics, Y9240L), 2 μL of 10 × Reaction Buffer and incubated at 30 °C for

(11) A Simple, Rapid, and Effective Heparinase Protocol to Enable Nucleic Acid Study from Frozen Heparinized PlasmaMethods and ProtocolsNovember 20, 2023Rownock Afruza, Nicole Minerva, Justin B. Lack, Moumita Chakraborty, James A. Haddad, Rabab O. Ali, Christopher Koh, Elliot B. Levy, Ohad Etzion, Theo HellerUltraPure™ DNase/RNase-Free distilled water were incubated with Bacteroides heparinase I (NEB, Cat# P0735L, Ipswich, MA, USA) enzyme, 10X heparinase buffer (supplied with the Bacteroides heparinase I enzyme,

(12) Plasma Small Extracellular Vesicle Cardiac miRNA Expression in Patients with Ischemic Heart Failure, Randomized to Percutaneous Intramyocardial Treatment of Adipose Derived Stem Cells or Placebo: Subanalysis of the SCIENCE StudyInternational Journal of Molecular SciencesJune 26, 2023Denise Traxler, Varius Dannenberg, Katrin Zlabinger, Alfred Gugerell, Julia Mester-Tonczar, Dominika Lukovic, Andreas Spannbauer, Ena Hasimbegovic, Jens Kastrup, Mariann GyöngyösiRNA was mixed with 4.5 μL mastermix, including UniSp6/CelmiR39 spike in control and 12U heparinase I (P0735L, New England Biolabs, Ipswich, MA, USA), as patients received heparin during the intervention. After

(13) Dynamic Changes in Heparan Sulfate Nanostructure in Human Pluripotent Stem Cell DifferentiationACS NanoApril 25, 2023Deena Al Al Mahbuba, Sayaka Masuko, Shiwei Wang, Deepsing Syangtan, Jeong Seuk Kang, Yuefan Song, Tay Won Shin, Ke Xia, Fuming Zhang, Robert J. Linhardt, et al.treated with a cocktail of heparinase enzymes composed of Bacteroides heparinase I (New England Biolabs, #P0735), heparinase II (New England Biolabs, #P0736), and heparinase III (New England Biolabs, #P0737). Each

(14) Cell Surface Nucleocapsid Protein Expression: A Betacoronavirus Immunomodulatory StrategybioRxiv : the preprint server for biologyFebruary 27, 2023López-Muñoz AD, Santos JJS, Yewdell JW.were treated with Bacteroides heparinase I (4.8 units), II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C. Cells were washed twice, incubated with recombinant proteins,

(15) Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunityScience advancesAugust 5, 2022López-Muñoz AD, Kosik I, Holly J, Yewdell JW.treated with Bacteroides heparinase I (4.8 U), II (1.6 U), and III (0.28 U) (New England Biolabs, nos. P0735S, P0736S, and P0737S) in DPBS for 1 hour at 30°C. Cells were washed twice, incubated with recombinant

(16) Distinct Stress‐Dependent Signatures of Cellular and Extracellular tRNA‐Derived Small RNAsAdvanced ScienceJune 1, 2022Guoping Li et al.cardiac surgery, the CPB patient plasma RNAs were pretreated with Heparinase I (New England Biolab, Cat# P0735L) at 30 ℃ for 3 h before DNase I treatment. Then, 100–500 ng His‐AlkB treated RNAs were used as input

(17) Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive ImmunitybioRxiv : the preprint server for biologyDecember 13, 2021López-Muñoz AD, Kosik I, Holly J, Yewdell JW.were treated with Bacteroides heparinase I (4.8 units), II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C. Cells were washed twice, incubated with recombinant proteins,

(18) Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive ImmunityResearch squareDecember 13, 2021Yewdell J, Lopez-Munoz A, Kosik I, Holly J.were treated with Bacteroides heparinase I (4.8 units), II (1.6 units) and III (0.28 units) (NEB # P0735S, # P0736S, # P0737S) in DPBS for 1 h at 30° C. Cells were washed twice, incubated with recombinant proteins,

(19) Heparan Sulfated Glypican-4 Is Released from Astrocytes by Proteolytic Shedding and GPI-Anchor Cleavage MechanismseNeuroJuly 1, 2021Huang K, Park S.luciferase assay. Heparinase treatment Samples containing Nluc-GPC4 were treated with Heparinase II (NEB P0735S) and Heparinase III (NEB P0737S) overnight at 37°C, then subject to DEAE pulldown described above, before

(20) Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in ObesityCell metabolismFebruary 2, 2021Brestoff JR, Wilen CB, Moley JR, Li Y, Zou W, Malvin NP, Rowen MN, Saunders BT, Ma H, Mack MR, et al.BioReagent",Sigma-Aldrich,Cat#H3149 Heparan sulfate proteoglycan,Sigma-Aldrich,Cat#H4777 Heparanase I,New England Biolabs,Cat#P0735S Heparanase II,New England Biolabs,Cat#P0736L Heparanase III,New England Biolabs,Cat#P0737L Humulin

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BacteroidesHeparinase I

BacteroidesHeparinase I from New England Biolabs

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BacteroidesHeparinase I from New England Biolabs

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