SpeI-HF R3133S from New England Biolabs

Product Specs
ItemSpeI-HF
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR3133S
Size500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Mapping of in vivo cleavage sites uncovers a major role for yeast RNase III in regulating protein-coding genesElifeMay 18, 2026Lee-Ann Notice-Sarpaning, Mathieu Catala, Catherine Stuart, Sherif Abou Elela, Ambro van Hoofcut with HindIII-HF (New England BioLabs, cat. R3104S, USA) and SpeI-HF (New England BioLabs, cat. R3133S, USA) restriction enzymes, and vector fragments were separated on a 1% agarose gel. The HYGR cassette

(2) Seipin-defined ER sites undergo distinct enrichment of factors for the biogenesis of sterol ester rich lipid dropletsiScienceMay 15, 2026Amit Khatri, Surabhi Yadav, Umair Hashmi, Atul Kumar Dandriyal, Vineet Choudhary,New England Biolabs,Cat# R3156S HindIII-HF,New England Biolabs,Cat# R3104S SpeI-HF,New England Biolabs,Cat# R3133S BamHI-HF,New England Biolabs,Cat# R3136S XhoI,New England Biolabs,Cat# R0146S TRIzol,Thermo Fisher Scientific

(3) Genetic interaction between Adgrg6 and Sox9 reveals a feedforward mechanism for postnatal spinal stabilityResearch SquareMay 4, 2026Valeria Aceves, Valeria Aceves, Cheng-Hai Zhang, Cheng-Hai Zhang, Jongkil Kim, Joshua Mathers, Andrew B. Mathers, Zhaoyang Lassar, Zhaoyang Gray(10X),Corning,20-031-CV Pronase,Roche,10 165 921 001 Sodium Hydroxide (NaOH),Fisher Chemical,S318-1 SpeI,NEB,R3133S TBE (10X),VWR,E442 Tricaine-methanesulfonate,Syndel,200–266 Tris-HCl,Sigma,T3253 TRIzol Reagent,Ambion

(4) Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translationNucleic Acids ResApril 30, 2026Dorthy Fang, John M Babich, Ryan Stanton, Isaac W Vock, Kyrillos Abdallah, Mingyi Zhu, Raj Letchuman, Richard Li, Matthew D Simon, Wendy V Gilbert, et al.plasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with SpeI-HF (NEB, R3133S) prior to in vitro transcription. In vitro transcription Short RNA oligos for motif randomization

(5) Genetic interaction between Adgrg6 and Sox9 reveals a feedforward mechanism for postnatal spinal stabilitybioRxivApril 8, 2026Valeria Aceves, Ziyi Xu, Cheng-Hai Zhang, Jongkil Kim, Joshua Ito, Kylie Mathers, Andrew B. Lassar, Zhaoyang Liu, Ryan S. Gray(10X),Corning,20-031-CV Pronase,Roche,10 165 921 001 Sodium Hydroxide (NaOH),Fisher Chemical,S318-1 SpeI,NEB,R3133S TBE (10X),VWR,E442 Tricaine-methanesulfonate,Syndel,200-266 Tris-HCl,Sigma,T3253 TRIzol Reagent,Ambion

(6) Miniature and versatile genome regulation TnpB-ωRNA toolkits facilitate cancer immunotherapyNature CommunicationsApril 1, 2026Junlin Lu, Jiajian Lai, Liang Cheng, Hengji Zhan, Kaiwen Jie, Chu Liu, Lin Huang, Meifeng Cen, Sen Liu, Zehua Chen, et al.were obtained from SYNBIO Technology. The pCDH vector was digested with SpeI-HF (New England Biolabs, R3133S) and SnaBI (New England Biolabs, R0130L), whereas the AAV2 vector was digested with NotI (New England Biolabs

(7) The mechanism of ribosomal recruitment during translation initiation on the Type 2 encephalomyocarditis virus IRESThe EMBO JournalMarch 18, 2026Sayan Bhattacharjee, Irina S Abaeva, Zuben P Brown, Yani Arhab, Hengameh Fallah, Christopher U T Hellen, Joachim Frank, Tatyana V PestovaT4 Polynucleotide Kinase,New England Biolabs,M0201S EcoRI,New England Biolabs,R0101S SpeI,New England Biolabs,R3133S BstNI,New England Biolabs,R0168S RNase T1,Thermo Fisher Scientific,EN0541 Isopropyl-beta-D-thiogalactoside,Gold Biotechnology

(8) N6-methyladenosine (m6A) modification of TXNIP in 3′UTR instigates abdominal aorta aneurysm in miceiScienceFebruary 20, 2026Fransky Hantelys, Wenfeng Yin, Ming Hui Zou,Synaptic Systems,Cat#202003 anti-YTHDF1,Proteintech,Cat#17479-1-AP anti-Phospho-4E-BP1 (Thr37/46),New England Biolabs,Cat#R3133S anti-YTHDF2,Proteintech,Cat#24744-1-AP anti-puromycin,Kerafast,Cat#EQ0001 anti-Colla1,Abcam,Cat#ab7046 anti-4EBP1,Abcam,Cat#ab278688 ,, "Chemicals,

(9) A non-catalytic role for RFC in PCNA-mediated processive DNA synthesisCellFebruary 19, 2026Gabriella N. L. Chua, Emily C. Beckwitt, Victoria Miller-Browne, Olga Yurieva, Dan Zhang, Bryce J. Katch, Nina Y. Yao, John W. Watters, Kaitlin Abrantes, Ryogo Funabiki, et al.,New England Biolabs,Cat# M0202S SphI-HF,New England Biolabs,Cat# R3182S SpeI-HF,New England Biolabs,Cat# R3133S ATP solution,ThermoFisher,Cat# R0441 Deoxynucleotide (dNTP) Solution Mix,New England Biolabs,Cat#

(10) A reversible allosteric inhibitor of GlyT2 for neuropathic pain without on-target side effectsNature CommunicationsFebruary 16, 2026Ryan P. Cantwell Chater, Julian Peiser-Oliver, Tanmay K. Pati, Ada S. Quinn, Irina Lotsaris, Zachary J. Frangos, Kristen E. Anderson, Anna E. Tischer, Billy J. Williams-Noonan, Karin R. Aubrey, et al.Facility (Sydney, Australia). Purified plasmid DNAs were linearised with SpeI (New England Biolabs, Cat#R3133S). Complementary RNA was transcribed by the T7 RNA polymerase using the mMESAGE mMACHINE T7 kit (Ambion,

(11) Changes in EGFR activity following CRISPR/Cas9-editing of the EGF binding domainScientific ReportsJanuary 31, 2026Jelena Popović, Anna Hahut, Gabriel E. Torres, Andrew Vincent, Nashaly Soto-Echevarria, Brian Wray, Elizabeth T. Bartom, Tatjana Paunesku, Chelain R. Goodman, Gayle E. WoloschakStates) and RFLP analysis was done using NcoI (R0193S, New England Biolabs, MA, United States) and SpeI (R3133S, New England Biolabs, MA, United States) enzymes. NcoI generated fragments of 259 and 246 bp while SpeI

(12) A commensal bacterium secretes effector proteins to establish population heterogeneity in the gutCell Host & MicrobeDecember 10, 2025Anna P. Zagieboylo, Ran Mo, Bentley Lim, Andrew L. Goodman,New England Biolabs,Cat#R3138S BamHI-HF,New England Biolabs,Cat#R3136S SpeI-HF,New England Biolabs,Cat#R3133S XbaI-HF,New England Biolabs,Cat#R0145S DpnI-HF,New England Biolabs,Cat#R0176S NEBuilder HiFi DNA Assembly Master Mix

(13) Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translationbioRxivDecember 8, 2025Dorthy Fang, John M. Babich, Ryan Stanton, Isaac W. Vock, Kyrillos Abdallah, Mingyi Zhu, Richard Li, Matthew D. Simon, Wendy V. Gilbert, Sigrid Nachtergaeleplasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with SpeI-HF (NEB, R3133S) prior to in vitro transcription. In vitro transcription Short RNA oligos for motif randomization

(14) Genome-scale CRISPR screen identifies host factors associated with bovine parainfluenza virus 3 infectionVirulenceDecember 2, 2025Yuanchen Geng, Chuanwen Jiang, Huaiyi Zhang, Hao Yang, Yongchong Peng, Yingyu Chen, Changmin Hu, Hailong Liu, Sheng Li, Huanchun Chen, et al.pTRIP-MCS-3Flag-IRES-RFP vector was digested with SpeI and SalI (New England Biolabs, Ipswich, MA, USA, Cat. No.: R3133S, R0138S) at 37°C for 2 h, and the digestion products were verified by 1% agarose gel electrophoresis

(15) Actin-binding protein tropomodulin-1 is necessary for basal constriction formation and bud detachment in Hydra vulgarisiScienceNovember 21, 2025Shravani Kulkarni, Shreya Maity, Niraj Tadasare, Bhagyashri Joshi, Ganesh Wagh, Swarnav Bhakta, Chinmoy PatraPolymerase,Takara,RR310Q T4 DNA Ligase,NEB,M0202S Restriction enzymes (SacII),NEB,R0157S Restriction enzymes (SpeI),NEB,R3133S SU5402,Sigma Aldrich,SML0443 U0126,Sigma Aldrich,662005 ATP,Sigma Aldrich,A26209 Rifampicin,SRL,R3501 Streptomycin,Sigma

(16) The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damageThe EMBO JournalNovember 20, 2025Alexandra Boegli, Elliott M Bernard, Louise Lacante, Gaël Majeux, Ella Hartenian, Vanessa Mack, Petr Brozfrom vectors described above and cloned into pINDUCER21 using the BstBI-SpeI (New England Biolabs #R3133S) restriction sites and In-Fusion cloning. Human NLRP3-mCherry constructs are derived from a pEGFP-C2-NLRP3

(17) Thermal-bias PCR: generation of amplicon libraries without degenerate primer interferencePeerJOctober 24, 2025Sean D. Mooredigestions were performed at 37 °C for 120 min using either BamHI-HF or SpeI-HF (NEB, cat. R3136S and R3133S) according to the manufacturer’s instructions. In preliminary restriction digestion studies, it was

(18) RABGAP1 is a sensor that facilitates the sorting and processing of amyloid precursor proteinThe EMBO JournalAugust 26, 2025Jessica Eden, Jonathan G G Kaufman, Conceição Pereira, Eleanor Fox, Jerome Cattin-Ortolá, Lorena Benedetti, Bart Nieuwenhuis, David J Owen, Jennifer Lippincott-Schwartz, Sean Munro, et al.under the EF1a promoter and separated by a T2A. pJEx226 was subsequently digested with SpeI-HF (NEB, R3133S) and MluI-HF (NEB, R3198S) to insert the RABGAP1 gene after a CAG promoter. To do this, the RABGAP1

(19) Ultrasound Control of Gene Expression in Human iPSCs via Heat Shock PromotersBiotechnology and BioengineeringAugust 20, 2025Alessandro R. Howells, Kama Bell, Hyeonu Heo, Tahir Haideri, Yun Jing, Xiaojun Lance LianWatertown, MA, USA) was restriction enzyme digested by KpnI and SpeI (New England Biolabs, #R3142S and #R3133S, respectively, Ipswich, MA, USA). The Cre insert was PCR amplified with Q5 High‐Fidelity 2X Master Mix

(20) Mapping of in vivo cleavage sites uncovers a major role for yeast RNase III in regulating protein-coding genesbioRxivAugust 13, 2025Lee-Ann Notice-Sarpaning, Mathieu Catala, Catherine Stuart, Sherif Abou Elela, Ambro van Hoofcut with HindIII-HF (New England BioLabs, cat. R3104S, USA) and SpeI-HF (New England BioLabs, cat. R3133S, USA) restriction enzymes and vector fragments were separated on a 1% agarose gel. The HYGR cassette

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5/8/2018Restriction Cloning Using SpeI-HF Triveni MahendrappaI have used SpeI-HF restriction enzyme from NEB to digest the plasmid called pSSPgl of a size 24 Kb. Digestion resulted in 23 and 1 Kb DNA fragments as seen in 0.7% agarose gel run. The 23 Kb DNA ... Read Review

SpeI-HF

SpeI-HF from New England Biolabs

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SpeI-HF from New England Biolabs

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