Ubiquitin N-Terminal Biotinylated Protein UB-560-050 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemUbiquitin N-Terminal Biotinylated Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $323.00Supplier Page View Company Product Page
Catalog NumberUB-560-050
Size50 ug
ApplicationsEnzyme Activity
Conjugate/TagBiotin
FormatSupplied as a solution in 10 mM HEPES pH 8.0
Purity>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Molecular Weight9 kDa
SpeciesHuman
Molecule NameUbiquitin
Source/Expression SystemE. coli-derived Recombinant Human Ubiquitin N-Terminal Biotin Protein, CF
NCBI Full Gene Nameribosomal protein S27a, ubiquitin B
NCBI Gene AliaseseS31, UBCEP1, S27A, HEL112, UBCEP80, HEL-S-50, UBC, CEP80, UBA80
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening

Specifications/Features

Available conjugates: Sizes Available: 50 ug

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Substitution of PINK1 Gly411 modulates substrate receptivity and turnoverAutophagyJune 1, 2023Fiesel FC, Fričová D, Hayes CS, Coban MA, Hudec R, Bredenberg JM, Broadway BJ, Markham BN, Yan T, Boneski PK, et al.incubated in 100 µl of buffer supplemented with 1 µg of N-terminally biotinylated Ub (Boston Biochem, UB-560-050), 1 mM TCEP (Goldbio, TCEP1), 2 mM ATP (Sigma-Aldrich, A1348), 0.01% Triton X-100 at 37°C for 24 h. For

(2) Ubiquitin Phosphorylation at Thr12 Modulates the DNA Damage ResponseMolecular cellNovember 5, 2020Walser F, Mulder MPC, Bragantini B, Burger S, Gubser T, Gatti M, Botuyan MV, Villa A, Altmeyer M, Neri D, et al.11484281001 Protein A Sepharose,Sino Biological Inc.,Cat# 10600-P07E-RN Biotin-ubiquitin,R&D Systems,Cat# UB-560–050 (Biotin-) pUbT12,This manuscript,N/A Fmoc-Gly trityl resin,Rapp Polymere GmbH,Cat# RA1213 PEG spacer,AK

(3) Age- and disease-dependent increase of the mitophagy marker phospho-ubiquitin in normal aging and Lewy body diseaseAutophagyJanuary 1, 2018Hou X, Fiesel FC, Truban D, Castanedes Casey M, Lin WL, Soto AI, Tacik P, Rousseau LG, Diehl NN, Heckman MG, et al.

(4) Heterozygous PINK1 p.G411S increases risk of Parkinson's disease via a dominant-negative mechanismBrain : a journal of neurologyJanuary 1, 2017Puschmann A, Fiesel FC, Caulfield TR, Hudec R, Ando M, Truban D, Hou X, Ogaki K, Heckman MG, James ED, et al.

(5) The E3 ligase HOIP specifies linear ubiquitin chain assembly through its RING-IBR-RING domain and the unique LDD extensionThe EMBO journalOctober 3, 2012Smit JJ, Monteferrario D, Noordermeer SM, van Dijk WJ, van der Reijden BA, Sixma TK.

(6) The PINK1 p.I368N mutation affects protein stability and ubiquitin kinase activityMolecular neurodegenerationJanuary 1, 0001"Ando M, Fiesel FC, Hudec R, Caulfield TR, Ogaki K, Górka-Skoczylas P, Koziorowski D, Friedman A, Chen L, Dawson VL, et al."

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Ubiquitin N-Terminal Biotinylated Protein from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Ubiquitin N-Terminal Biotinylated Protein from R&D Systems, a Bio-Techne Brand

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