Recombinant Human Ubiquitin Protein, CF U-100H-10M from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Human Ubiquitin Protein, CF
CompanyR&D Systems, a Bio-Techne Brand
Price $184.00Supplier Page View Company Product Page
Catalog NumberU-100H-10M
Size10 mg
ApplicationsEnzyme Activity
Conjugate/TagUnconjugated
FormatLyophilized from a solution in deionized water
Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Molecular Weight8.6 kDa
SpeciesHuman
Molecule NameUbiquitin
Source/Expression SystemE. coli-derived Recombinant Human Ubiquitin Protein, CF
NCBI Full Gene Nameubiquitin B
NCBI Gene AliasesHEL-S-50
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) A triple-action PROTAC for wild-type p53 cancer therapyCell Reports MedicineDecember 16, 2025Gregory H. Bird, Utsarga Adhikary, Michael J. Schmidt, Marina Godes, Bethany Tesar, Christina M. Camara, Joao A. Paulo, Julia F. Vidlak, Thomas M. DeAngelo, Marilyn Marquez, et al.(E−305-025), 1 μM UbcH5c/UBE2D3 (E2-627-100), 10 mM MgATP (B-20), and 100 μM human ubiquitin protein (U-100H-10M). Recombinant FLAG-p53 (20 μM, BPS Bioscience, 100412) and recombinant 6xHis-BRD4 bearing BD1 and BD2

(2) Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimeraNat CommunDecember 11, 2025Jin Du, Elisia Villemure, Matthew Johnson, Caleigh Azumaya, Catarina J. Gaspar, Scot Marsters, David Lawrence, Scott Foster, Alexis Rohou, Tommy K. Cheung, et al.were visualized by IB with the IRE1 antibody. UBE2D1 (E2-616-100), UBE2D2 (E2-622-100), ubiquitin (U-100H-10M), methylated ubiquitin (U-502-01M), and NEDD8 (UL-812-500) were purchased from Biotechne. Detection

(3) A modular Uba1-nanobody fusion enables selective ubiquitin transfer to tagged E2 enzymesJournal of Biological ChemistryDecember 1, 2025Charlotte Wijne, Francesca D'Amico, David A. Pérez Berrocal, Monique P.C. Mulder, Hidde L. Ploegh, Jin Gan(1 μM) were incubated with 1 μM of one of the tested E2s and 20 μM of human ubiquitin (R&D Systems #U-100H-10M) in a 100 mM Tris–HCl buffer supplemented with 5 mM MgCl2. After set-up of the reaction mixture, 15

(4) Midkine attenuates amyloid-β fibril assembly and plaque formationNature Structural & Molecular BiologyNovember 1, 2025Masihuz Zaman, Shu Yang, Ya Huang, Jay M. Yarbro, Yanhong Hao, Zhen Wang, Danting Liu, Kiara E. Harper, Hadeer Soliman, Alex Hemphill, et al.concentration in the absence and presence of human Ub protein (5 and 10 µM, Bio-Techne R & D systems, U-100H-10M) to monitor aggregation kinetics as described above. Aggregation kinetics of MDK and Ub at different

(5) Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivationNature structural & molecular biologySeptember 1, 2025Zhang L, Gordiyenko Y, Morgan T, Franco C, Tufegdžić Vidaković A, Zhang S.

(6) Molecular basis of SIFI activity in the integrated stress responseNatureJuly 1, 2025Yang Z, Haakonsen DL, Heider M, Witus SR, Zelter A, Beschauner T, MacCoss MJ, Rapé M.

(7) Glucosidase alpha neutral C promotes influenza virus replication by inhibiting proteosome-dependent degradation of hemagglutininSignal Transduct Target TherApril 23, 2025Xinzhong Liao, Qian Xie, Minqi Liang, Qijun Liao, Bi Huang, Shengze Zhang, Feng Zhang, Liangliang Wang, Lifang Yuan, Xuejie Liu, et al.(E-305-025, R&D Systems), 400 ng of UbcH5c/UBE2D3 (E2-627, R&D Systems), and 5 μg of recombinant ubiquitin (U-100H-10M, R&D Systems) at 30 °C for 1 h. The supernatant was removed, and the reaction was terminated by adding

(8) The unique structure of the highly conserved PPLP region in HIV-1 Vif is critical for the formation of APOBEC3 recognition interfacesmBioMarch 12, 2025Iwatani Y, Matsuoka K, Ode H, Kubota M, Nakata Y, Setoyama Y, Kojima K, Imahashi M, Yokomaku Y.

(9) A positive feedback loop between SMAD3 and PINK1 in regulation of mitophagyCell DiscovMarch 11, 2025Mingzhu Tang, Dade Rong, Xiangzheng Gao, Guang Lu, Haimei Tang, Peng Wang, Ning-Yi Shao, Dajing Xia, Xin-Hua Feng, Wei-Feng He, et al.SMAD3 protein (Med Chem Express, HY-P71323), Recombinant Human Ubiquitin Protein, CF (R&D systems, Cat# U-100H-10M). Primary antibodies used in this study anti-PINK1 (Cell Signaling Technology, Cat# 6946), anti-phospho-ubiquitin

(10) Reprograming of the ubiquitin ligase Ubr1 by intrinsically disordered Roq1 through cooperating multifunctional motifsThe EMBO JournalFebruary 7, 2025Niklas Peters, Sibylle Kanngießer, Oliver Pajonk, Rafael Salazar Claros, Petra Hubbe, Axel Mogk, Sebastian Schuck(Bio-Techne),UM-NOK-01M Recombinant human ubiquitin (K0),MoBiTec,E1610-UBP Recombinant human ubiquitin (WT),R&D Systems (Bio-Techne),U-100H-10M Recombinant luciferase,Promega,E1702 D-(+)-Trehalose dihydrate,Sigma-Aldrich,T9531-10G Tunicamycin,Sigma-Aldrich,654380-50MG Software,Software,Software ImageJ,2.0.0-rc-69/1.52n,National

(11) SFXN2 contributes mitochondrial dysfunction-induced apoptosis as a substrate of ParkinFrontiers in cellular neuroscienceJanuary 1, 2025Luo S, He Y, He Y, Wang D.

(12) The RING-type E3 ligase RIE1 sustains leaf longevity by specifically targeting AtACS7 to fine-tune ethylene production in ArabidopsisProceedings of the National Academy of Sciences of the United States of AmericaNovember 26, 2024Tang X, Mei Y, He K, Liu R, Lv X, Zhao Y, Li W, Wang Q, Gong Q, Li S, et al.

(13) Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopyScientific reportsNovember 7, 2024Vukovic D, Winkelvoß D, Kapp JN, Hänny AC, Bürgisser H, Riermeier L, Udovcic A, Tiefenboeck P, Plückthun A.

(14) Clr4(SUV39H1) ubiquitination and non-coding RNA mediate transcriptional silencing of heterochromatin via Swi6 phase separationNature communicationsOctober 30, 2024Kim HS, Roche B, Bhattacharjee S, Todeschini L, Chang AY, Hammell C, Verdel A, Martienssen RA.

(15) Ubiquitin-mediated degradation of SlPsbS regulates low night temperature tolerance in tomatoesCell reportsOctober 22, 2024Lu J, Yu J, Liu P, Gu J, Chen Y, Zhang T, Li J, Wang T, Yang W, Lin R, et al.

(16) Promotion of DNA end resection by BRCA1-BARD1 in homologous recombinationNatureOctober 1, 2024Salunkhe S, Daley JM, Kaur H, Tomimatsu N, Xue C, Raina VB, Jasper AM, Rogers CM, Li W, Zhou S, et al.

(17) Ubiquitylation of nucleic acids by DELTEX ubiquitin E3 ligase DTX3LEMBO ReportsSeptember 6, 2024Kang Zhu, Chatrin Chatrin, Marcin J Suskiewicz, Vincent Aucagne, Benjamin Foster, Benedikt M Kessler, Ian Gibbs-Seymour, Dragana Ahel, Ivan Aheldescribed (Zhu et al, 2023; Zhu et al, 2022). UBE1 (E-304-050), UBCH5A (E2-616-100), and recombinant Ub (U-100H-10M) were purchased from R&D Systems. Exo T nuclease (M0265S) was purchased from NEB. Oligonucleotides Single-stranded

(18) Bivalent target-binding bioPROTACs induce potent degradation of oncogenic SHP2The Journal of biological chemistrySeptember 1, 2024Hoffman M, Krum D, Wittrup KD.

(19) Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteinsNature communicationsJuly 10, 2024Chan A, Haley RM, Najar MA, Gonzalez-Martinez D, Bugaj LJ, Burslem GM, Mitchell MJ, Tsourkas A.

(20) VHL suppresses UBE3B-mediated breast tumor growth and metastasisCell death & diseaseJune 24, 2024Wang S, Li H, Liu X, Yin T, Li T, Zheng M, Liu M, Meng X, Zhou J, Wang Y, et al.

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Recombinant Human Ubiquitin Protein, CF from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Recombinant Human Ubiquitin Protein, CF from R&D Systems, a Bio-Techne Brand

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