Human SCF, premium grade 130-096-695 from Miltenyi Biotec

(1) CD117 epitope-shielded hematopoietic stem cell transplantation with toxin-free conditioning and in vivo selection ameliorates a β-thalassemia modelbioRxivJuly 8, 2026Romina Marone, Rosalba Lepore, Kiriaki Paschoudi, Jessica Zuin, Alessandro Sinopoli, Anna Camus, Thomas Burgold, Ewelina Bartoszek, Diego Calabrese, Mylène Toranelli, et al.(StemSpan SFEM II (StemCell, 09655) supplemented with 100 ng/ml human stem cell factor (hSCF) (Miltenyi, 130–096-695), 100 ng/ml human FMS-like tyrosine kinase ligand (hFlt3)-ligand (Miltenyi, 130–096-479), 100 ng/ml

(2) Transposable elements shape stemness in normal and leukemic hematopoiesisNat GenetMay 4, 2026Giacomo Grillo, Bettina Nadorp, Aditi Qamra, Bryce Drylie, Amanda Mitchell, Christopher Arlidge, Ankita Nand, Naoya Takayama, Alex Murison, Seyed Ali Madani Tonekaboni, et al.(Thermo Fisher Scientific, 35050061), Primocin (0.1 mg ml−1; InvivoGen), SCF (200 ng ml−1; Miltenyi Biotech, 130-096-696), IL3 (20 ng ml−1; Miltenyi Biotech, 130-095-069), TPO (20 ng ml−1; Peprotech, 300-18), FLT3L (40 ng

(3) Tracking tau and cellular responses in human iPSC-microglia: from uptake to seedable secretion, including in extracellular vesiclesAlzheimers DementApril 6, 2026Maria Kreger Karabova, Anna Del Ser-Badia, Anne Hedegaard, Sam J. Washer, Zeynep Baykam, Darragh P. O'Brien, Iolanda Vendrell, Svenja S. Hester, Roman Fischer, Errin Johnson, et al.vascular endothelial growth factor (Catalog No. PHC9394, Peprotech), and 20 ng/mL SCF (Catalog No. 130‐096‐695, Miltenyi Biotec) to promote embryoid body (EB) formation directed to mesoderm and hemogenic endothelium.

(4) Targeting p75NTR activity alleviates the neurotoxic effect of high glucose on iPSC-derived dopaminergic neuronsStem Cell Research & TherapyMarch 21, 2026Konstantina Chanoumidou, Ioanna Zota, Maria Anna Papadopoulou, Chrystalla Konstantinou, Alexandros Tsimpolis, Electra Tsagliotis, Maria Tziortziou, Katerina Ntarntani, Anne Grünewald, Matthieu David Lavigne, et al.ng/mL BMP4 (Invitrogen, PHC9534), 50 ng/mL VEGF (Invitrogen, PHC9394), and 20 ng/mL SCF (Miltenyi, 130-096-695) supplemented with 10 µM Rock inhibitor (Abcam Biochemicals, Ab120129) on the day of plating. Medium

(5) microRNA-132 attenuates inflammation in induced pluripotent stem cell-derived microglia from Alzheimer’s disease patientsActa Neuropathologica CommunicationsMarch 7, 2026Amber Penning, Sarah Snoeck, Olmo Ruiz Ormaechea, Dilara Ayyildiz, Oliver Polzer, Martin Buitrago-Arango, Raffaella Capobianco, Fred de Winter, Sriram Balusu, Joost Verhaagen, et al.Thermo Fisher Scientific), 50 ng/mL Human VEGF-165 Recombinant Protein (Cat#100–20, Peprotech), and 20 ng/mL Human SCF (Cat#130-096-693, Miltenyi Biotec). After four days, EBs were transferred to 6-well cell culture plates at a density

(6) Chemically Defined, Efficient Megakaryocyte Production from Human Pluripotent Stem CellsCellsNovember 20, 2025Kim JE, Lee Y, Kim Y, Hwang SB, Choi YB, Han J, Jung J, Song JW, Joung JG, Ko JJ, et al.(R&D systems) Retinoic acid,"Sigma(St. Louis, MO, USA)",R2625,HY-14649G (MedChemExpress) SCF,Miltenyi,130-096-695,170-076-149 IL-3,Miltenyi,130-095-069,170-076-110 VPA,Sigma,PHR1061,1708707 "Penicillin-Streptomycin,

(7) Parallel Evolution of Leukemic Clones in Myeloproliferative NeoplasmsResearch SquareNovember 17, 2025Tyler M. Challen, Aishwarya Krishnan, Infencia Xavier Krishnan, Infencia Xavier Young, David R. O'Leary, David O'Leary, Maggie Arand, Stephen T. Allen, Grant A. Challenpenicillin-streptomycin (Fisher Scientific #MT30002CI), 50 ng/mL human stem cell factor (SCF; Miltenyi Biotec #130–096–695), 50 ng/mL human thrombopoietin (TPO; Miltenyi Biotec #130–094–013), and 50 ng/mL human FLT3L (Miltenyi Biotec

(8) The Parkinson's disease-associated LRRK2-G2019S variant restricts serine metabolism, leading to microglial inflammation and dopaminergic neuron degenerationJ NeuroinflammationOctober 27, 2025Henry Kurniawan, Sarah L. Nickels, Alise Zagare, Elisa Zuccoli, Isabel Rosety, Gemma Gomez-Giro, Enrico Glaab, Jens C. Schwamborngrowth factor 165 (VEGF-165, Biolegend, 583708), and 20 ng/ml stem cell factor (SCF, Miltenyi Biotec, 130–096–695). EBs were harvested and transferred to ultra-low attachment 6-well plates (ThermoFisher Scientific,

(9) Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell cultureOpen Research EuropeSeptember 19, 2025Lukasz Truszkowski, Sveva Bottini, Sara Bianchi, Mirko G. Scrivano, Giulia Ferrari Ramondo, Linda Belluci, Helen Bell, Silvia Becca, Kirsten E Snijders, Giulia Savorè, et al.(Peprotech, #100-64), 25 ng mL -1 of IGF1 (Miltenyi Biotec, 130-093-887) and SCF (Miltenyi Biotec, #130-096-696). EBs were cultured for a total of 10 days. Throughout the differentiation process, cells were maintained

(10) Nanobioreactor detection of space-associated hematopoietic stem and progenitor cell agingCell Stem CellSeptember 4, 2025Jessica Pham, Jane Isquith, Larisa Balaian, Shuvro P. Nandi, Claire Engstrom, Karla Mack, Inge van der Werf, Patrick Chang, Jana Stoudemire, Luisa Ladel, et al.Media,Gibco,Cat# 10639011 MEM alpha,Gibco,Cat# 12571063 Stem Cell Factor (SCF),Miltenyi Biotec,Cat# 130-096-693 Thrombopoietin (TPO),Miltenyi Biotec,Cat# 130-095-747 FLT-3,Miltenyi Biotec,Cat# 130-096-476 IL-6,Miltenyi Biotec

(11) Tafazzin regulates neutrophil maturation and inflammatory responseEMBO ReportsFebruary 17, 2025Przemysław Zakrzewski, Christopher M Rice, Kathryn Fleming, Drinalda Cela, Sarah J Groves, Fernando M Ponce-Garcia, Willem Gibbs, Kiran Roberts, Tobias Pike, Douglas Strathdee, et al.(ACD),Sigma-Aldrich,8013-89-6 Human Flt3-ligand,Miltenyi Biotec,130-096-479 Human SCF,Miltenyi Biotec,130-096-695 Recombinant human IL-3,Bio-Techne,203-IL-010/CF Human GM-SCF,Miltenyi Biotec,130-093-864 Human G-CSF,Miltenyi Biotec

(12) Selective pressures of platinum compounds shape the evolution of therapy-related myeloid neoplasmsNature CommunicationsJuly 17, 2024Eline J. M. Bertrums, Jurrian K. de Kanter, Lucca L. M. Derks, Mark Verheul, Laurianne Trabut, Markus J. van Roosmalen, Henrik Hasle, Evangelia Antoniou, Dirk Reinhardt, Michael N. Dworzak, et al.StemSpan SFEM medium (Stemcell technologies, Cat#09650) supplemented with SCF (100 ng/mL; Miltenyi Biotec Cat#130-096-696), Flt3-ligand (100 ng/mL; Miltency Biotec Cat#130-096-480), IL-6 (20 ng/mL; Stem Cell Technologies Cat#78050),

(13) BRD9 regulates normal human hematopoietic stem cell function and lineage differentiationCell Death & DifferentiationJuly 1, 2024Swati Garg, Wei Ni, Basudev Chowdhury, Ellen L. Weisberg, Martin Sattler, James D. Griffinsupplemented with cytokines purchased from Miltenyi at a final concentration of 100 ng/mL rh-SCF (#130-096-695), 100 ng/mL rh-FL(#130-096-479), 50 ng/mL rh-TPO(#130-095-752), 100 µM β-mercaptoethanol (Gibco #21985023),

(14) Multimodal cartography of human lymphopoiesis reveals B and T/NK/ILC lineages are subjected to differential regulationiScienceOctober 20, 2023Alhaj Hussen K, Chabaane E, Nelson E, Lekiashvili S, Diop S, Keita S, Evrard B, Lardenois A, Delord M, Verhoeyen E, et al."Human M-CSF, premium grade",Miltenyi Biotech,Cat#130-096-492 "Human SCF, premium grade",Miltenyi Biotech,Cat#130-096-695 "Human TPO, premium grade",Miltenyi Biotech,Cat#130-095-752 "Human IL-21, premium grade",Miltenyi Biotech

(15) microRNA-132 regulates gene expression programs involved in microglial homeostasisiScienceJune 16, 2023Hannah Walgrave, Amber Penning, Giorgia Tosoni, Sarah Snoeck, Kristofer Davie, Emma Davis, Leen Wolfs, Annerieke Sierksma, Mayte Mars, Taofeng Bu, et al.(Cat#PHC9531, Thermo Fisher Scientific), 50 ng/mL VEGF (Cat#100–20, Peprotech), and 20 ng/mL SCF (Cat#130-096-693, Miltenyi Biotec). Next, four-day EBs were differentiated in 6-well plates (approximately 15 EBs/well)

(16) Distinct Assemblies of Heterodimeric Cytokine Receptors Govern Stemness Programs in LeukemiaCancer DiscovMay 16, 2023Winnie L. Kan, Urmi Dhagat, Kerstin B. Kaufmann, Timothy R. Hercus, Tracy L. Nero, Andy G.X. Zeng, John Toubia, Emma F. Barry, Sophie E. Broughton, Guillermo A. Gomez, et al.(Thermo Fisher Scientific, 35050061), Primocin 0.1 mg/mL (Invitrogen), SCF (200 ng/mL; Miltenyi Biotec, 130-096-696), IL3 (20 ng/mL; Miltenyi Biotec, 130-095-069), TPO (20 ng/mL; PeproTech, 300-18), FLT3L (40 ng/mL;

(17) Single-cell transcriptomics defines an improved, validated monoculture protocol for differentiation of human iPSC to microgliaSci RepNovember 14, 2022Sam J. Washer, Marta Perez-Alcantara, Yixi Chen, Juliette Steer, William S. James, Gosia Trynka, Andrew R. Bassett, Sally A. Cowley(PeproTech no PHC9534), 50 ng/mL VEGF (PeproTech no PHC9394), and 20 ng/mL SCF (Miltenyi Biotec no 130-096-695)) supplemented with 10 µM Y-27632 ROCK inhibitor. iPSC were cultured to 70–80% confluency before washing

(18) Inhibition of Complex I of the Respiratory Chain, but Not Complex III, Attenuates Degranulation and Cytokine Secretion in Human Skin Mast CellsInternational Journal of Molecular SciencesSeptember 30, 2022Thomas Buttgereit, Moritz Pfeiffenberger, Stefan Frischbutter, Pierre-Louis KrauÃŸ, Yuling Chen, Marcus Maurer, Frank Buttgereit, Timo GaberUSA). IL-4 (20 ng/mL, Peprotech Ref#200-04-100) and stem cell factor (SCF, 100 ng/mL, Miltenyi Ref#130-096-696) were added twice a week. The >95% purity of cultured CD117/FcεRI-positive skin MCs was routinely confirmed

(19) Whole-genome sequencing and mutational analysis of human cord-blood derived stem and progenitor cellsSTAR protocolsJune 17, 2022Rosendahl Huber A, van Leeuwen AJCN, Peci F, de Kanter JK, Bertrums EJM, van Boxtel R.proteins","Chemicals, peptides, and recombinant proteins" ,, Human Stem Cell Factor (SCF),Miltenyi Biotec,Cat#130-096-696 Human Flt3 – Ligand,Miltenyi Biotec,Cat#130-096-480 Interleukin-6 (IL-6),Stem Cell Technologies,Cat#78050 Interleukin-3

(20) CDK7/12/13 inhibition targets an oscillating leukemia stem cell network and synergizes with venetoclax in acute myeloid leukemiaEMBO molecular medicineApril 7, 2022He L, Arnold C, Thoma J, Rohde C, Kholmatov M, Garg S, Hsiao CC, Viol L, Zhang K, Sun R, et al.cultured in IMDM supplemented with 20% BIT (Stem Cell Technologies #09500), SCF 100 ng/ml (Miltenyi #130‐096‐695), FLT3L 100 ng/ml (Miltenyi #130‐096‐479), TPO 50 ng/ml (Miltenyi #130‐095‐752), β‐mercaptoethanol 10−4

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Human SCF, premium grade from Miltenyi Biotec

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Human SCF, premium grade from Miltenyi Biotec

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