T4 RNA Ligase 2, truncated KQ M0373S from New England Biolabs

Product Specs
ItemT4 RNA Ligase 2, truncated KQ
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberM0373S
Size2000 units
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Conserved but mechanistically diverse piRNA defence against endogenous retroviruses in insectsEMBO ReportsApril 2, 2026Shashank Chary, Patricia E Carreira, Sarah Nicholas, Kathryn B McNamara, Ian A Cockburn, Karin Nordström, Therésa M Jones, Rosalyn Gloag, Alyson Ashe, Leon E Hugo, et al.Biosciences,NU-1619-CY5 Terminal Deoxynucleotidyl Transferase,Thermo Fisher,EP0161 "T4 RNA Ligase 2, K227Q",New England Biolabs,M0373L T4 RNA Ligase 1,New England Biolabs,M0204S SuperScript II Reverse Transcriptase,Thermo Fisher,18064014 KAPA

(2) Exosome-mediated decay of unstable long extended precursors of human telomerase RNA is dependent on 5′-cap trimethylationGenes & DevelopmentApril 1, 2026Aniruddha Samajdar, Roberta Amato, Seongmoon Jo, Ethan Cordes, Albert Tai, Grazia Daniela Raffa, Luis F.Z. BatistaNEB S1315S, sequence: 5′-rAppCTGTAGGCACCATCAAT–NH2-3′) with 250 U of T4 RNA ligase-truncated KQ (NEB M0373S), 25% PEG 8000, and 1 µL of RNaseOUT (Invitrogen 10777019) in a 20 µL reaction for 16 h at 25°C. Ligated

(3) RNA functional modulation by Mitoxantrone via RNA structural ensemble repartitioningNature CommunicationsMarch 23, 2026Chundan Zhang, Ivana Borovská, Teona Iobashvili, Edoardo Morandi, Marta Lionnez, Oluwatosin S. Olayinka, Rinse de Boer, Massimiliano Clamer, Martin D. Witte, Klaus Pors, et al.Data 6), at 25 °C for 2 h, in a final volume of 20 μl, using 200 U T4 RNA Ligase 2, truncated KQ (cat. M0373L, New England Biolabs), in T4 RNA Ligase Reaction Buffer supplemented with 12.5% PEG-8000. Ligated RNA

(4) Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cellsNucleic Acids ResMarch 14, 2026Neesha Kara, Laura Biggins, Alex Whale, Kieron May, Vera Grinkevich, Paola Garran-Garcia, Jhanavi Srinivasan, Peter J Rugg-Gunn, Claudia Ribeiro de Almeida, Samantha J Walker, et al.8000, 1 µl 10 pM/µl TrAEL-seq adaptor 1 with multiplex index and 1 µl T4 RNA ligase 2 truncated KQ (NEB M0373L). Plugs were then rinsed with 1 ml Tris buffer, transferred to individual 15 ml tubes and washed three

(5) Cell-free DNA methylome and fragmentome analysis for relapse monitoring of Ewing sarcomaEMBO Molecular MedicineMarch 6, 2026Sophie A Richardson, Aram Safrastyan, Mina Karimpour, Gayatri Gulati, Patrick J B Harker, Alan Redfern, Simon P Pearce, Vsevolod J Makeev, Bernadette Brennan, Alexander T J Lee, et al.Thermosensitive Phosphatase,Thermo Fisher,EF0654 "T4 RNA Ligase 2, Truncated KQ",New England Biolabs,M0373L KAPA HiFi HotStart PCR Kit,KAPA Biosystems (Roche),KK2502 / 7958897001 Invitrogen SuperScript IV Reverse Transcriptase

(6) Characterization of gRNA-dependent and gRNA-independent off-target binding sites of PspCas13b and RfxCas13d in mammalian cellsNucleic Acids ResearchFebruary 23, 2026Huanhuan Feng, Zhixue Li, Hongdao Zhang, Yuli Zheng, Beiying Xu, Yao Zhang, Lin Zou, Ligang Wu2.5 µl 3′ adapter (20 µM), 1 µl RiboLock RNase inhibitor, 1.5 µl T4 RNA Ligase 2, truncated KQ (NEB, M0373), 6 µl 50% PEG8000, 16 µl water] and incubated in a thermomixer at 25°C for 2.5 h with an intermittent

(7) Structural modification of oxazolidinone antibiotics alters nascent peptide stalling preference and peptide trajectory through the ribosomebioRxivFebruary 21, 2026Jordan I. Kleinman, Tushar Raskar, Dorota Klepacki, Teresa Szal, Nora Vázquez-Laslop, Alexander S. Mankin, James S. Fraser, Danica Galonić Fujimori50 μM Universal miRNA cloning linker (NEB S1315S) using 200 U of T4 RNA ligase 2 truncated KQ (NEB M0373L) for 3 h at 37°C. Ligated products of appropriate size range (32-62 nt) were isolated from 15% TBE Urea

(8) Step-by-Step Protocol for In Situ Profiling of RNA Subcellular Localization Using TATA-seqBio ProtocFebruary 20, 2026Xiao Jiang, Chu Xu, Lulu HuT4 polynucleotide kinase (NEB, catalog number: M0201L) 25. T4 RNA ligase 2 truncated KQ (NEB, catalog number: M0373) 26. M-MLV reverse transcriptase (Promega, catalog number: M1705) 27. NEBNext Ultra II Q5 master mix

(9) Step-by-Step Protocol for In Situ Profiling of RNA Subcellular Localization Using TATA-seqBIO-PROTOCOLFebruary 20, 2026Xiao Jiang, Chu Xu, Lulu HuT4 polynucleotide kinase (NEB, catalog number: M0201L) 25. T4 RNA ligase 2 truncated KQ (NEB, catalog number: M0373) 26. M-MLV reverse transcriptase (Promega, catalog number: M1705) 27. NEBNext Ultra II Q5 master mix

(10) Coronaviruses reprogram the tRNA epitranscriptome to favor viral protein expressionNature CommunicationsFebruary 19, 2026Elena Muscolino, Mireia Puig-Torrents, Jaime Buigues Bisquert, Diogo Correa Mendonca, Marc Talló-Parra, Gemma Perez-Vilaro, Omar Caño-Prades, Gavin R. Meehan, Karen Kerr, Vanessa Herder, et al.with a 3’ dideoxycytidine) were ligated to the purified tRNA using T4 RNA ligase 2 truncated KQ (NEB M0373L), in a reaction containing 50% PEG-8000, T4 RNA ligase buffer (10X), and RNase inhibitor (SUPERase•In,

(11) Mechanistic insights into RNA chaperoning by Ro60 and La autoantigensCellFebruary 19, 2026Hyeyeon Nam, Justin C. Deme, Soyeong Sim, Marco Boccitto, Susan M. Lea, Sandra L. Wolinlibrary was prepared as described103, except that T4 RNA ligase 2 (truncated KQ) (New England Biolabs M0373S) was used for the first adaptor ligation and SuperScript III (Thermo Fisher 18080044) was used for reverse

(12) Systematic Identification of Germ Granule Proteins Reveals Specialized Roles in RNAi and Small RNA InheritancebioRxivFebruary 1, 2026Shihui Chen, Lucas H. Prescott, Craig C. Mello, Carolyn M. Phillipspolyphosphatase (Epicenter RP8092H) and ligated to 3’ pre-adenylated adapters with Truncated T4 RNA ligase (NEB M0373L). Small RNAs were then hybridized to the reverse transcription primer, ligated to the 5’ adapter with

(13) Genome-wide profiling of RNA 2’-O-methylation in neurons and identification of orphan snoRNA targetsResearch SquareJanuary 20, 2026Gordon Carmichael, Xuan Ye, Yaling Liu, Yinzhou Zhu, Saran Alshawi, Brittany Elliott, Christopher Holley, Jean-Denis Beaudoin, Brenton R. Graveleyincubating for 30 minutes. A 3’-DNA linker was ligated to the RNA with T4 RNA ligase 2 truncated KQ (NEB, M0373S). Then a 5’-RNA linker was ligated to the RNA. The linker was denatured and then the ligation reaction

(14) RNA G-quadruplexes promote codon repeat-associated ribosomal frameshifting in human genesNucleic Acids ResearchJanuary 16, 2026Xiuwen Li, Zhanbiao Li, Yingshui Zhou, Shimin Gong, Zhenjing Chen, Jingyang Li, Miaomiao Guo, Xiaoqian Gu, Fei Li, Jiayu Wei, et al.NEB, M0201L). Pre-adenylated 5′ adapters were joined to the RPFs via T4 RNA ligase 2 (25°C, 2 h; NEB, M0373L). Ligated products were isolated using streptavidin magnetic beads. cDNA synthesis was carried out with

(15) High-throughput functional profiling and evolutionary covariation analysis of entire riboswitch sequencesbioRxivJanuary 16, 2026Laura M. Hertz, Anibal Arce, Elena Rivas, Julius B. LucksSuperaseIn RNAse Inhibitor (Invitrogen, AM2696), 0.5 μL of T4 RNA Ligase, and 0.5 μL of Trunc KQ (NEB, M0373L) was added and mixed by pipetting up and down 10 times. A master mix according to the number of samples

(16) DEAD-box ATPase–marked condensates coordinate compartmentalized translation and antibiotic persistenceSci AdvJanuary 2, 2026Ziyin Zhang, Daqian Li, Bo Zheng, Jia-feng Liuwater and subjected to ligation to a 5′-adenylated DNA adaptor using truncated T4 RNA ligase 2 (NEB, M0373) at room temperature for 3 hours. Reverse transcription was carried out using SuperScript IV (Thermo Fisher Scientific

(17) Genome-wide profiling of RNA 2’- O -methylation in neurons and identification of orphan snoRNA targetsbioRxivJanuary 1, 2026Xuan Ye, Yaling Liu, Yinzhou Zhu, Sarah A. Alshawi, Brittany A. Elliott, Christopher L. Holley, Jean-Denis Beaudoin, Brenton R. Graveley, Gordon G. Carmichaelincubating for 30 minutes. A 3’-DNA linker was ligated to the RNA with T4 RNA ligase 2 truncated KQ (NEB, M0373S). Then a 5’-RNA linker was ligated to the RNA. The linker was denatured and then the ligation reaction

(18) RG motifs promote piRNA-mediated gene silencing in C. elegansNucleic Acids ResDecember 11, 2025Dylan C Wallis, Carolyn M Phillips(NEB M0356S) (IP samples) and ligated to 3′ pre-adenylated adapters with truncated T4 RNA ligase (NEB M0373L). Small RNAs were then hybridized to the reverse transcription primer, ligated to the 5′ adapter with

(19) CRISPR-free RNA base editing mediated PTC-readthrough restores hearing in mice with Otof nonsense mutationNature CommunicationsDecember 6, 2025Hanxiao Sun, Qi Teng, Wenqing Liu, Rui Guo, Menghua Li, Wei Xiong, Qiang Huang, Qianru Yu, Nan Luo, Yang Li, et al.the 3′ RNA adapter (5′-rAPP-AGATCGGAAGAGCGTCGTG-3SpC-3′) was carried out using T4 RNA ligase 2 (NEB, M0373S) at 25 °C for 2 h, followed by enzymatic digestion with 5’deadenylase (NEB, M0331S) and RecJf (NEB,

(20) Integrated analysis of transcriptome, sRNAome and degradome sequencing provides insights into bacterial wilt resistance in potatoBMC Plant BiologyDecember 5, 2025Yu Yang, Xiaoyuan Zhang, Jiaping Ma, Jun Xiao, Zhengxiang Feng, Junmei Yu, Wanjie Li, Pengfei Jiang, Guangtao Zhu, Yupeng Geng, et al.RNAs were ligated to pre-adenylated 3′ adaptors by T4 RNA ligase 2, truncated KQ (NEW ENGLAND Biolabs, M0373L), annealed with the reverse transcription primer, and then ligated to 5′ RNA adaptors using T4 RNA ligase

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T4 RNA Ligase 2, truncated KQ

T4 RNA Ligase 2, truncated KQ from New England Biolabs

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T4 RNA Ligase 2, truncated KQ from New England Biolabs

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