Thermostable 5 AppDNA/RNA Ligase M0319S from New England Biolabs

Product Specs
ItemThermostable 5 AppDNA/RNA Ligase
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberM0319S
Size10 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiaeJournal of Visualized ExperimentsFebruary 27, 2026Lokha Ranjani Alagar Boopathy, Leena P. Sen, Leonard Schärfen, Karla M. NeugebauerT4 Polynucleotide Kinase,New England Biolabs,M0201S Thermostable 5’ App DNA/RNA Ligase,New England Biolabs,M0319L "Tris-HCl, pH 7.4, 1M",Thermo Scientific,J60202.K2 Triton X-100,TEKNOVA,T1105 TRIzol™ Reagent,Invitrogen,15596-026 β-mercaptoethanol,Sigma

(2) Lysosomal RNA profiling reveals targeting of specific types of RNAs for degradationbioRxivSeptember 16, 2025G. Jordan Ray, Eleonora Nardini, Heather R. Keys, Daniel H. Lin, David M. Sabatini, David P. Bartel(/5Phos/NNNNNNNNNNGATCGTCGGACTGTAGAACTCTGAACGTGTAG/3SpC3/) using Thermostable 5’ AppDNA/RNA ligase (NEB M0319S). DNA was cleaned up using MinElute reaction cleanup kit. cDNA was amplified using Phusion (Thermo Fisher

(3) Rapid folding of nascent RNA regulates eukaryotic RNA biogenesisMolecular CellApril 17, 2025Leonard Schärfen, Isaac W. Vock, Matthew D. Simon, Karla M. Neugebauer,New England Biolabs,M0297L Mth RNA Ligase,New England Biolabs,M2611A Thermostable 5’ App DNA/RNA Ligase,New England Biolabs,M0319L KAPA HiFi HotStart PCR Kit,Roche,07958889001 Dynabeads™ Oligo(dT)25,Thermo Fisher Scientific,61002 T4 Polynucleotide Kinase

(4) tRNA regulation and amino acid usage bias reflect a coordinated metabolic adaptation in Plasmodium falciparumiScienceNovember 15, 2024Qian Li, Leonie Vetter, Ylva Veith, Elena Christ, Ákos Végvári, Cagla Sahin, Ulf Ribacke, Mats Wahlgren, Johan Ankarklev, Ola Larsson, et al.RNA ligase,New England Biolab,Cat#M2611A Thermostable 5′ AppDNA/RNA ligase,New England Biolab,Cat#M0319L Potassium chloride,Sigma,Cat#P9541 2-(N-morpholino)ethanesulfonic acid (MES),Sigma,Cat#M3671 Magnesium chloride

(5) Mesoscale DNA features impact APOBEC3A and APOBEC3B deaminase activity and shape tumor mutational landscapesNature communicationsMarch 18, 2024Sanchez A, Ortega P, Sakhtemani R, Manjunath L, Oh S, Bournique E, Becker A, Kim K, Durfee C, Temiz NA, et al.incubated overnight at 60 °C (Ligation tests with Thermostable 5′ APP DNA/RNA Ligase [New England BioLabs, #M0319] and T4 RNA Ligase 2 Truncated KQ [New England BioLabs, #M0373] were performed as indicated by the manufacturer

(6) XACT-seq: A photocrosslinking-based technique for detection of the RNA polymerase active-center position relative to DNA in Escherichia coliSTAR ProtocolsDecember 17, 2021Chirangini Pukhrambam, Irina O. Vvedenskaya, Bryce E. Nickels8,Thermo Fisher Scientific,Cat#AM9732 Taq DNA polymerase,NEB,Cat#M0273 5′ App DNA/RNA ligase,NEB,Cat#M0319S T4 RNA Ligase 1 (ssRNA Ligase),NEB,Cat#M0204L Phusion HF DNA polymerase,Thermo Fisher Scientific,Cat#F-530L "Phusion HF Buffer Pack, detergent-free

(7) XACT-Seq Comprehensively Defines the Promoter-Position and Promoter-Sequence Determinants for Initial-Transcription PausingMolecular CellSeptember 1, 2020Jared T. Winkelman, Chirangini Pukhrambam, Irina O. Vvedenskaya, Yuanchao Zhang, Deanne M. Taylor, Premal Shah, Richard H. Ebright, Bryce E. NickelsMagneHis Ni-Particles,Promega,V8560 DNase I,Zymo Research,E1009A T4 RNA Ligase 1 (ssRNA Ligase),NEB,M0204L 5’ App DNA/RNA ligase,NEB,M0319S T4 Polynucleotide Kinase (PNK),NEB,M0201S Superscript III Reverse Transcriptase,Thermo Fisher,18080–044 RNAse

(8) Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)Journal of Visualized ExperimentsJuly 30, 2019Lauren Woodward, Pooja Gangras, Guramrit SinghT4 Polynucleotide Kinase-2500U,NEB,M0201L, T4RNL2 Tr. K227Q,NEB,M0351S, Tetracycline,Sigma,87128, Thermostable 5´ App DNA/RNA Ligase,NEB,M0319S, TruSeq_SE1 5′-pGGCACTANNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′,Any,,this

(9) Oxidative Stress Triggers Selective tRNA Retrograde Transport in Human Cells during the Integrated Stress ResponseCell reportsMarch 19, 2019Schwenzer H, Jühling F, Chu A, Pallett LJ, Baumert TF, Maini M, Fassati A.Kit,InGex,Cat #TGIRTKit25 5′DNA Adenylation Kit,NEB,Cat# E2610S Thermostable 5′ AppDNA/RNA Ligase,NEB,Cat# M0319S Phusion High Fidality PCR Master Mix,Life Technologies,Cat# F-531S NextSeq® 500/550 Mid Output Kit

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Thermostable 5 AppDNA/RNA Ligase

Thermostable 5 AppDNA/RNA Ligase from New England Biolabs

New England Biolabs

Citations (9)

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Thermostable 5 AppDNA/RNA Ligase from New England Biolabs

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