FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and Goat F(ab’)2 Anti-Human IgG, Mouse ads-FITC (SB Cat. No. 2043-02). Biotin and FITC conjugated antibodies were detected with serially diluted Neutralite Avidin-PE (SB Cat. No. 7200-09).